Abstract
Eighty-four selected lines from a recombinant inbred (RI) line population of 284 lines derived from a cross between the indica varieties IR20 and IR55178-3B-9-3 were used in a hydroponic culture experiment with sufficient P supply (10 mg P L−1) and P-deficient stress (0.05 mg P L−1). After 2 weeks, the activity of acid phosphatase (AAP) in roots of each parent and each line from both normal culture and P-deficient stress was determined. QTLs for AAP, P-deficiency stress induced AAP (Psi-AAP) and relative AAP (RAAP) were detected using 178 molecular markers mapped on all 12 chromosomes based on single marker analysis and interval mapping. One QTL for AAP and three QTLs for Psi-AAP were detected on chromosome 1, 6 and 12, respectively. Two QTLs for RAAP were identical with these for Psi-AAP on chromosome 6 and 12. The results in this case indicated that the genetic system for Psi-AAP was different with that for AAP under normal culture. The AAP was mainly influenced by interaction among muti-factors, while Psi-AAP was controlled by a Psi genetic system.
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References
Besford R T 1979a Nutrient imbalance in tomato plants and acid phosphatase activity in the leaves. J. Sci. Food Agric. 30, 275–280.
Besford R T 1980 A rapid tissue test for diagnosing phosphorus deficiency in the tomato plants. Ann. Bot. 45, 225–227.
Besford R T 1979b Phosphorus nutrition and acid phoaphatase activity in the leaves of seven plant species. J. Sci. Food Agric. 30, 281–285.
Boultin J, Provot M and Roux L 1981 Effect of cycloheximide and renewal of phosphorus supply on surface acid phosphatase activity of phosphorus deficient tomato plant. Physiol. Plant. 51, 353–360.
Bradfold M M 1976 A rapid and sensitive method for the quantitation of microgram quantities of utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Chang C W and Bandurski R S 1964 Exocellular enzymes of corn roots. Plant Physiol. 39, 60–64.
Chaubey C N, Senadhira D and Gregorio G B 1994 Genetic analysis of tolerance to phosphorous deficiency in rice (Orazy sativa L.). Theory Appl. Genet. 89, 313–317.
Fageria N K, Wright R J and Baligar V C 1988 Rice cultivar evalution for phosphorous-use efficiency. Plant Soil 111, 105–109.
Goldstein A H, Baertlein D A and Mcdaniel R G 1988 Phosphate starvation inducible metabolism in Lycopersicon esculentum, I. Excretion of acid Phosphatase by tomato plants and suspensioncultured. Plant Physiol. 87, 711–715.
Gunawardena S D I E and Wijeratne H M S 1978 Screening of rice under phosphorous-deficiency soil in Sri Lanka. Int. Rice Conf. (No.34), pp 245–252. International Rice Research Institute, Los. Banos., The Philippines.
Hayashi T and Takijima 1953 Biochemical studies on the metabolism in roots of crop plants. II. Relation between phosphorus nutrition and phosphatase activity of crop roots. J. Sci. Soil Manure 24, 17–20
Hung H H 1985 Studies on tillering ability of rice under phosphorus stress. PhD thesis. A&M University, Texas.
Ikehashi H and Ponnamperuma F N 1978 Varietal tolerance of rice for adverse soils. In: Soil and Rice. Symp IRRI. pp 801–823. Los. Banos., Philippines. IRRI 1971 Annual report for 1970. Los. Banos., Philippines.
IRRI 1976 Annual report for 1975. Los. Banos., Philippines.
IRRI 1996 Annual report for 1995. Los. Banos., Philippines.
Jackson M L 1958 Soil Chemical Analysis. Prentice Hall, Inc., Englewood Cliffs, NJ.
Kaneko Y, Tamai Y, Toh-e A and Oshima Y 1985 Transcriptional and posttranscriptional control of PHO8 expression by PHO regulatory gene in Saccharomyces cerevisiae. Mol. Cell Biol. 5, 248–252.
Lander E S 1993 Mapmaker/Exp 3.0 and Mapmaker/QTL 1.1, tutorial and reference manual. Whitehead Institute, 9 Cambridge Center, Cambridge, Massachusetts.
Lynch J P and Brown K M 1999 Regulation of root architecture by phosphorus availability. In Phosphorus in Plant Biology: Regulatory Roles in Molecular, Cellular, Organisimic and Ecosystem Processes. Eds. Lynch JP and Deikman J. pp 148–156. American Society of Plant Physiologist, Maryland (1999).
Majumder N D, Borthakur D N and Rakshit S C 1989 Heterosis in rice under phosphorous stress. India J. Genet. 49, 231–235.
Mchlan K D and Mirco D D 1982 Acid phosphatase activity of intact roots and phosphorus nutrition in plants. III. Its relation to phosphorus garnering by wheat and its comparison with leaf activity as measure of phosphorus status. Aust. J. Agric. Res. 33, 1–11.
Ni J J, Wu P, Senadhira D and Huang N 1998 Mapping of QTLs for phosphorus deficiency tolerance in rice (Oryza sativa L.). Theor. Appl. Genet. 97, 1361–1369.
Rao N N, Wang E, Yashphe J and Torriani A 1986 Nucleotide pool in Pho regulon mutants and alkaline Phosphatase systhesis in Escherichia coli. J. Bacteriol. 165, 205–211.
Senanayake N 1984 Varietal tolerance to phosphorous deficiency in wetland rice soils. Trop. Agric. 14, 67–78.
Tabatabai M A and Bremner J M 1969 Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 1, 301–307.
Torriani A and Ludtke D N 1985 The pho regulon of Escherichia coli. In The Molecular Biology of Bacteria Growth. Ed. M Schaechter. pp 224–242. Jones and Bartlett Publishers, Boston.
Wang D L, Zhu J, Li Z K and Paterson A 1999 Mapping QTLs with epistatic and QTL _ environmental interactions by mixed linear model approaches. Theor. Appl. Genet., in press.
Wissuwa M, Yano M and Ae N 1998 Mapping of QTLs for phosphorus-deficiency tolerance in rice (Oryza sativa L.). Theor. Appl. Genet. 97, 777–783.
Wu P, Hu B, Liao C Y, Zhu J M, Wu Y R, Senadhira D and Paterson A H 1998 Characterization of tissue tolerance to iron by molecular marker in different line of rice. Plant Soil 203, 217–226.
Wu P and Ni J J 2000 Detecting QTLs for Phosphorus Uptake and Use Efficiency in Rice Using AFLP and RFLP Markers. Acta Bot. Sinica 42, 229–233.
Yoshida K, Kouromitsu Z, Ogawa N, Ogawa K and Oshima Y 1987 Phosphorus metabolism and cellular regulation. In Microorganisms.Ed. Torrinia-Gorini A, Rothman FG, Silver S, Wright A and Yagil E. pp 49–55. American Society for Microbiology, Washington.
Yoshida S, Forno D A, Cock J H and Gomez K A 1976 Laboratory manual for physiological studies of rice, 3rd edn. The International Rice Research Institute, Manila.
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Hu, B., Wu, P., Liao, C. et al. QTLs and epistasis underlying activity of acid phosphatase under phosphorus sufficient and deficient condition in rice (Oryza sativa L.). Plant and Soil 230, 99–105 (2001). https://doi.org/10.1023/A:1004809525119
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DOI: https://doi.org/10.1023/A:1004809525119