Abstract
The absence of a rapid and inexpensive method for estimating macroinvertebrate individual biomass is a limit to the study of freshwater communities, given that traditional methods are time- and money-consuming. We propose an inexpensive method to quickly estimate individual biomass from video-recorded images of pond macroinvertebrates. We used a software that automatically measures several body dimensions (area, perimeter, minor and major axes) on each individual and linear regression to relate these body dimensions to dry weight. Area was found to be the best predictor of dry weight. The method allows individual measures of macroinvertebrates at a reasonable speed and accuracy, and may be useful for biomass size spectra or secondary production studies.
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Benke, A. C., A. D. Huryn, L. A. Smock & J. B. Wallace, 1999. Length-mass relationships for freshwater macroinvertebrates in North America with particular reference to the Southeastern United States. J. n. am. Benthol. Soc. 18: 308–343.
Bourassa, N. & A. Morin, 1995. Relationships between size structure of invertebrate assemblages and trophy and substrate composition in streams. J. n. am. Benthol. Soc. 14: 393–403.
Dumont, H. J., I. Van de Velde & S. Dumont, 1975. The dry weight estimate of biomass in a selection of Cladocera, Copepoda and Rotifera from the plankton, periphyton and benthos of continental waters. Oecologia 19: 75–97.
Hanson, J. M., E. E. Prepas & W. C. Mackay, 1989. Size distribution of the macroinvertebrate community of a freshwater lake. Can. J. Fish. aquat. Sci. 46: 1510–1519.
Ramsay, P. M., S. D. Rundle, M. J. Attrill, M. G. Uttley, P. R. Williams, P. S. Elsmere & A. Abada, 1997. A rapid method for estimating biomass size spectra of benthic metazoan communities. Can. J. Fish. aquat. Sci. 54: 1716–1724.
Rasmussen, K. J., 1993. Patterns in the size structure of littoral zone macroinvertebrate communities. Can. J. Fish. aquat. Sci. 50: 2192–2207.
Sheldon, R. W., A. Prakash & W. H. Sutcliffe, 1972. The size distribution of particles in the ocean. Limnol. Oceanogr. 17: 327–383.
Solimini, A. G., A. Ruggiero, M. Anello, A. Mutschlechner & G. Carchini, 2000. The benthic community structure in mountain ponds affected by livestock watering in nature reserves of central Italy. Verh. int. Ver. Limnol. 27: 501–505.
Strayer, D., 1986. The size structure of a lacustrine zoobenthic community. Oecologia 69: 513–516.
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Bernardini, V., Solimini, A.G. & Carchini, G. Application of an image analysis system to the determination of biomass (ash free dry weight) of pond macroinvertebrates. Hydrobiologia 439, 179–182 (2000). https://doi.org/10.1023/A:1004153703748
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DOI: https://doi.org/10.1023/A:1004153703748