Abstract
Due to the importance of common bean angular leaf spotin the state of Minas Gerais-Brazil and to the greatvariability of the pathogen, Phaeoisariopsisgriseola, monitoring races becomes an important toolfor breeding programs aiming at genetic resistance.The pathogenic variability of 30 isolates of the P. griseola, collected from various locations in thestate of Minas Gerais, was studied using the followingcommon bean differential series (Don Timóteo,Bolón Bayo, Montcalm, G 5686, Amendoin, G 11796,BAT 332, PAN 72, Cornell 49-242, México 54, Florde Mayo and G 2858). The first trifoliate leaf wasinoculated with a 2 × 104 conidia/mL. Plants weremaintained at 20–22 °C and 95% relativehumidity for 48 hours. Symptom evaluation wasperformed 15 days after inoculation. Thirteen raceswere identified demonstrating the wide geneticvariability of the pathogen in the state of MinasGerais. Race 63.63 was the most virulent, whereas race63.23 was the most frequent (10 of 30 isolates), beingwidely distributed among the regions studied. Thevirulence phenotype indicated that the races studiedbelonged to the Mesoamerican group, which wasconfirmed when the 30 isolates were compared to Andeanand Mesoamerican standards using RAPD markers.
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References
Alvarez-Ayala, G. & H.F. Schwartz, 1979. Preliminary investigations of pathogenic variability expressed by Isariopsis griseola. Annual Report Bean Improvement Cooperation 22: 86-88.
Aparício, B.H.E., 1998. Caracterizacion de la diversidad molecular y la virulencia de aislamentos del hongo Phaeoisariopsis griseola de Brasil y Bolívia. Universidad del Valle — Facultad de Ciencias, Cali, Colômbia, pp. 120.
Brenes, B.M., G.M. Chaves & L. Zambolim, 1983. Estimativas de perdas no rendimento do feijoeiro comum (Phaseolus vulgaris L.) causadas pela mancha angular (Isariopsis griseola Sacc.) Fitopatologia Brasileira 8: 599.
Brock, R.D., 1951. Resistance to angular leaf spot among varieties of beans. Journal of the Australian Institute of Agriculture Science 17: 25-30.
Buruchara, R.A., 1983. Determination of pathogenic variation in Isariopsis griseola Sacc. and Pseudomonas syringae pv. Phaseolicola (Burk., 1926) Young, Dye and Wilkie 1978. Nairobi: Universidade de Nairobi, 1983. pp. 188. Tese (Doutorado em Agronomia). Nairobi, Universidade de Nairobi.
Correa-Victoria, F.J., 1987. Pathogenic Variation, Production of Toxic Metabolites, and Isoenzyme Analysis in Phaeoisariopsis griseola (Sacc.) Ferr. Ph D diss Michigan State University, pp. 154.
Kado, C.I. & M.G. Heskett, 1970. Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas and Xanthomonas. Phytopathology 60: 969-976.
Lacerda, J.T., R.S.B. Coelho & R.L.R. Mariano, 1994. Variabilidade patogênica de Isariopsis griseola em feijoeiro no estado de Pernambuco. Summa Phytopathologica 20: 93-96.
Nei, M. & W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings National Academy Science USA 76: 5269-5273.
Pastor-Corrales, M.A. & C.E. Jara, 1995. La evolución de Phaeoisariopsis griseola con el frijol comun en América Latina. Fitopatología Colombiana 19: 15-22.
Pastor-Corrales, M.A. & H.F. Schwartz, 1994. Problemas de Produccion del Frijol en los Trópicos. 2. ed. Cali, Colômbia: Centro Internacional de Agricultura Tropical, pp. 73.
Pyndji, M.M., 1976. Variabilité pathogénique de Phaeoisariopsis griseola dans la region des Grands Lacs. In: P. Nyabyenda & U. Scheidegger (Eds.), Actes du Sixieme Séminaire Regional sur Amélioration du Haricot dans la region des Grands Lacs. Kigali, Rwanda: (African Workshop Series, 17).
Pyndji, M.M., 1993. Pathogenic variability of Phaeoisariopsis griseola in the Great Lakes region. In: PAN African Bean Pathology Working Group Meeting, 1992, Thinka, Kenya. Proceedings... Thinka, Kenya: (CIAT African Workshop Series, 23).
Raeder, V. & P. Broda, 1987. Rapid preparation of DNA from filamentous fungi. Letters Applied Microbiology 1: 17-20.
Ribeiro, M.J., R.S.B. Coelho & M.A. Menezes, 1993. Fontes de resistência em feijoeiro (Phaseolus vulgaris L.) à mancha angular do feijoeiro (Isariopsis griseola Sacc). Caderno Ômega — Série Agronomia 1: 243-246.
Sartorato, A. & C.A. Rava, 1984. Especialização fisiológica de Isariopsis griseola Sacc. em Phaseolus vulgaris L. Summa Phytopathologica 10: 58-59.
Sartorato, A., C.A. Rava & J.O.M. Menten, 1991. Resistência vertical do feijoeiro comum (Phaseolus vulgaris L.) a Isariopsis griseola Sace. Fitopatologia Brasileira 16: 43-46.
Singh, S.P., P. Gepts & D. Debouck, 1991. Races of common bean (Phaseolus vulgaris, Fabaceae). Economic Botany 45: 379-396.
Van Schoonhoven, A. & M.A. Pastor-Corrales, 1987. (Comps.). Standard System for Evaluation of Bean Germplasm. Cali, Colombia: CIAT, 54 pp.
Villegas, J.M., 1959. Variabilidad del Isariopsis griseola Sacc. agente causal de la mancha angular del frijol (Phaseolus vulgaris L.). Colômbia: Manizales Universidade de Caldas, 1959. 61 pp. Dissertação (Mestrado em Agronomia) — Manizales Universidade de Caldas.
Wilkinson, L., M. Hill, J.P. Welna et al., 1992. Statistics. Chicago: Systat, 750 pp.
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Nietsche, S., Borém, A., Assis de Carvalhos, G. et al. Genetic diversity of Phaeoisariopsis griseola in the State of Minas Gerais, Brazil. Euphytica 117, 77–84 (2001). https://doi.org/10.1023/A:1004096421990
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DOI: https://doi.org/10.1023/A:1004096421990