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Levels, Phosphorylation Status and Cellular Localization of Translational Factor EIF2 in Gastrointestinal Carcinomas

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Abstract

The level of expression and the phosphorylation status of the α subunit of initiation factor 2 (eIF2α) protein have been determined by comparing samples from human stomach, colon and sigma-rectum carcinomas with normal tissue from the same patients. The unphosphorylated and phosphorylated levels of cytoplasmic eIF2α, as well as the percentage of phosphorylated factor over the total, were significantly higher in stomach, colon and sigma-rectum tumours compared with normal tissue. The expression of this factor was also studied by using immunocytochemical methods, where redistribution towards the nucleus in tumour cells as compared with normal tissue was observed. Our results support a likely implication of eIF2α in gastrointestinal cancer.

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Authors and Affiliations

  1. Departamento de Investigación, Hospital Ramón y Cajal, 28034, Madrid, Spain

    María V.T. Lobo, M. Isabel Pérez, F. Javier M. Alonso & Matilde Salinas

  2. Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Alcalá de Henares, 28071, Madrid, Spain

    M. Elena Martín

  3. Servicio de Anatomía Patológica, Hospital Ramón y Cajal, 28034, Madrid, Spain

    Clara Redondo & M. Isabel Álvarez

Authors
  1. María V.T. Lobo
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  2. M. Elena Martín
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  3. M. Isabel Pérez
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  4. F. Javier M. Alonso
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  5. Clara Redondo
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  6. M. Isabel Álvarez
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  7. Matilde Salinas
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Lobo, M.V., Martín, M.E., Pérez, M.I. et al. Levels, Phosphorylation Status and Cellular Localization of Translational Factor EIF2 in Gastrointestinal Carcinomas. Histochem J 32, 139–150 (2000). https://doi.org/10.1023/A:1004091122351

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