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Utilization of isozyme polymorphism for cultivar identification of 45 commercial peas (Pisum sativum L.)

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Abstract

Forty five Pisum sativum cultivars were analysed by isozyme electrophoresis with the objective to find protein markers for exact and reproducible discrimination of individual genotypes. The combination of six enzyme systems (acid phosphatase, amylase, esterase, leucine aminopeptidase, shikimate dehydrogenase and phosphoglucomutase) with two electrophoretic techniques (NATIVE-PAGE, isoelectric focusing) and use of seed and leaf tissue enabled to identify all 45 studied cultivars. Critical factors which may affect utilization of isozyme electrophoresis for commercial applications in pea breeding and seed production and testing are discussed.

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Authors
  1. Zdeněk Pošvec
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  2. Miroslav Griga
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Pošvec, Z., Griga, M. Utilization of isozyme polymorphism for cultivar identification of 45 commercial peas (Pisum sativum L.). Euphytica 113, 249–256 (2000). https://doi.org/10.1023/A:1003967315213

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