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A reproducible protocol for the production of gynogenic haploids of mulberry, Morus alba L.

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Abstract

Gynogenic haploids of a female clone of mulberry (Morus alba L. Cv. K-2) were produced by unpollinated ovary culture. Segments from in vitro developed inflorescences were cultured on MS+ BAP (8.5 μM)+2,4-D (4.5 μM) and after three weeks individual ovaries were transferred to MS+2,4-D (4.5 μm)+glycine (6660 μM)+proline (1738 μM). In this treatment sixteen per cent of the ovaries developed a gynogenic seedling. The gynogenic plants have been established in soil. Out of twenty gynogenic plants examined cytologically, twelve showed the haploid number of chromosomes (2n=x=14) and the other eight were aneuploids, with 13–17 chromosomes in their root tip cells.

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References

  • Bhojwani, S.S. & M.K. Razdan, 1996. Plant Tissue Culture: Theory and Practice, A Revised Edition. Elsevier, Amsterdam, 767 pp.

    Google Scholar 

  • Jain, A.K., A. Sarkar & R.K. Datta, 1996. Induction of haploid callus and embryogenesis in in vitro cultured anthers of Mulberry (Morus indica). Plant Cell Tissue Organ Cult 44: 143-147.

    Article  Google Scholar 

  • Lakshmi Sita, G. & S. Ravindran, 1991. Gynogenic plants from ovary cultures of mulberry (Morus indica). In: J. Prakash & R.L.M. Pierik (Eds), Horticulture: New Technologies and applications, pp. 225-229. Kluwer, Dordrecht.

    Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.

    Article  CAS  Google Scholar 

  • San Noeum, L.H., 1976. Haploids d Hordeum vulgare L. par culture in vitro non fecondes. Ann Amelior Plant 26: 751-754.

    Google Scholar 

  • Sethi, M., S. Bose, A. Kapur, & N.S. Rangaswamy, 1992. Embryo differentiation in anther cultures of mulberry. Ind J Exp Biol 30: 146-148.

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi-, 110 007, India

    T. Dennis Thomas, A.K. Bhatnagar, M.K. Razdan & S.S. Bhojwani

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  1. T. Dennis Thomas
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  2. A.K. Bhatnagar
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  3. M.K. Razdan
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  4. S.S. Bhojwani
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Dennis Thomas, T., Bhatnagar, A., Razdan, M. et al. A reproducible protocol for the production of gynogenic haploids of mulberry, Morus alba L.. Euphytica 110, 169–173 (1999). https://doi.org/10.1023/A:1003797328246

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  • DOI: https://doi.org/10.1023/A:1003797328246

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