Abstract
PCR-RFLP analysis was conducted to clarify the relationship between the leaf color of progenies and their ptDNA inheritance in interspecific three-way crosses, (Rhododendron kiusianum × R. eriocarpum) × R. japonicum f. flavum. All albino progenies contained maternal ptDNA, whereas green and pale-green progenies contained paternal ptDNA. Sectorial chimeric progenies, of which the leaf and shoot color was turned from green to albino during the culture, contained both maternal and paternal ptDNA in green segments and maternal ptDNA in albino segments. These results suggest that albino progenies are caused by the incompatibility between plastome from F1 hybrids of R. kiusianum × R. eriocarpum and nuclear genome from R. japonicum f. flavum.
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References
Akabane, M., A. Yamanaka, D. Takashima, T. Nakatsue & Y. Nakamura, 1971. On the fertility of interspecific crossing and the growth of F1 seedlings in Rhododendron species. Bull Tochigi Pref Agri Expt St 15: 95-102 (In Japanese).
Anderson, W.C., 1984. A revised tissue culture medium for shoot multiplication of rhododendron. J Amer Soc Hort Sci 109: 343-347.
Corriveau, J.L. & A.W. Coleman, 1988. Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm species. Amer J Bot 75: 1443-1458.
Hagemann, R., 1992. Plastid genetics in higher plants. In: R.G. Herrmann (Ed), Cell organelles, pp 65-96. Springer, Wien, New York.
Heursel, J., 1981. Diversity of flower colors in Rhododendron simsii Planch and prospect for breeding. Euphytica 30: 9-14.
Johnson, L.A. & D.E. Soltis, 1994. MatK DNA sequences and phylogenic reconstruction in Saxifragaceae s.str. Systematic Botany 19: 143-156.
Kirk, J.T.O. & R.A.E. Tilney-Bassett, 1978. The plastids, their chemistry, structure, growth and inheritance, 2nd edn. Elsevier/ North Holland, Amsterdam.
Kobayashi, N., 1996. Studies on phylogeny of Rhododendron L. and origin of azalea cultivars. Doctor Thesis, University of Tsukuba (In Japanese).
Kobayashi, N., R. Takeuchi, T. Handa & K. Takayanagi, 1995. Cultivar identification of evergreen azalea with RAPD method. J Japan Soc Hort Sci 64: 611-616.
Neuhaus, H. & G. Link, 1987. The chloroplast tRNALys (UUU) gene from mustard. Curr Genet 11: 251-257.
Noguchi, Y., 1932. Studies on the species crosses of Japanese Rhododendron. I. On the crossability between various species and the cotyledon color of F1 seedlings. Japan J Bot 6: 103-124.
Ureshino, K., I. Miyajima & M. Akabane, 1998. Effectiveness of three-way crossing for the breeding of yellow-flowered evergreen azalea. Euphytica 104: 113-118.
Yamaguchi, S., M. Kunishige & T. Tamura, 1985. Interspecific compatibility in Japanese Rhododendrons. Bull Veg & Ornam Crop Res Stn Japan Ser B 8: 87-97 (In Japanese with English Summary).
Yao, J.L., D. Cohen & R.E. Rowland, 1994. Plastid DNA inheritance and plastome-genome incompatibility in interspecific hybrids of Zantedeschia (Araceae). Theor Appl Genet 88: 255-260.
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Ureshino, K., Miyajima, I., Ozaki, Y. et al. Appearance of albino seedlings and ptDNA inheritance in interspecific hybrids of azalea. Euphytica 110, 61–66 (1999). https://doi.org/10.1023/A:1003754004737
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DOI: https://doi.org/10.1023/A:1003754004737