Abstract
The genetic basis of resistance to powdery mildew (Erysiphe graminis DC. f.sp. hordei Marchal) was analyzed using doubled haploid barley (Hordeum vulgare L.) lines from the cross Harrington/TR306. Based on infection types observed after inoculation with defined single-conidium isolates, the lines were classified into four groups. The observed phenotypic ratio fit a two-locus model. The two putative loci were mapped relative to molecular markers. One coincided with the previously mapped dMlg locus on chromosome 4. Based on the observed infection types, Harrington carries the Mlg resistance allele, and TR306 carries a second locus on chromosome 7 (5H); this was tentatively designated Ml(TR). It is the first reported race-specific powdery mildew resistance gene located on that chromosome. These two loci were also detected by simple interval mapping of disease severity data from naturally infected field plots. Composite interval mapping with the first two resistance loci as co-factors detected an additional locus on chromosome 6, with a minor effect on resistance. Finally, superimposing the race-specific classification onto the field data provided evidence for a minor-effect locus on chromosome 7 (5H). The Mlg locus had the largest effect, the Ml(TR) locus had an intermediate effect and the other two loci had very small effects. This study demonstrates the effectiveness of an integrated approach to identifying and mapping resistance loci using classification data from inoculated experiments and quantitative data from field experiments.
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References
Backes, G., A. Graner, B. Foroughi-Wehr, G. Fishbeck, G. Wenzel & A. Jahoor, 1995. Localization of quantitative trait loci (QTL) for agronomicly important characters by the use of a RFLP map in barley (Hordeum vulgare L.). Theor. Appl. Genet. 90: 294–302.
Falak, I. & D.E. Falk, 1993. Doubled haploids as a tool for studying resistance to powdery mildew (Erysiphe graminis f.sp. hordei). Barley Newsletter 36: 208.
Falak I., 1994. Analysis of a doubled haploid population for resistance to powdery mildew. M.Sc. Thesis. University of Guelph, Guelph, Ont. Canada. 168 pp.
Haley, C.S. & S.A. Knott, 1992. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69: 315–324.
Hayes, P.M., B.H. Liu, S.J. Knapp, F. Chen, B.L. Jones, T. Blake, J. Franckowiak, D. Rasmusson, M. Sorrells, S.E. Ullrich, D. Wesenberg & A. Kleinhofs, 1993. Quantitative trait locus effects and environmental interaction in a sample of North American barley germ plasm. Theor. Appl. Genet. 87: 392–401.
Heun, M., 1987. Genetics of quantitative resistance in barley against Erysiphe graminis f.sp. hordei. In: S. Yasuda & T. Konishi (Eds), Barley Genetics V, pp. 593–600. Okayama University Press, Japan.
Heun, M., 1992. Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map. Genome 35: 1019–1025.
Jørgensen, J.H., 1987. Three kinds of powdery mildew resistance in barley. In: S. Yasuda & T. Konishi (Eds.), Barley Genetics V, pp. 583–592. Okayama University Press, Japan.
Jørgensen, J.H., 1994. Genetics of powdery mildew resistance in barley. CRC Critical Reviews in Plant Sciences. 13: 97–119.
Kasha, K.J., A. Kleinhofs, A. Kilian, M. Saghai Maroof, G.J. Scoles, P.M. Hayes, F.Q. Chen, X. Xia, X.-Z. Li, R.M. Biyashev, D. Hoffman, L. Dahleen, T.K. Blake, B.G. Rossnagel, B.J. Steffenson, P.L. Thomas, D.E. Falk, A. Laroche, W. Kim, S.J. Molnar & M.E. Sorrells, 1995. The North American barley map on the cross HT and its comparison to the map on cross SM. In: K. Tsunewaki (Ed.), The Plant Genome and Plastome: Their Structure and Evolution, pp. 73–88. Kodansha Scientific Ltd., Tokyo.
Kleinhofs, A., A. Kilian, M.A. Saghai Maroof, R.M. Biyashev, P. Hayes, F.Q. Chen, N. Lapitan, A. Fenwick, T.K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, D. Kudrna, J. Bollinger, S.J. Knapp, B. Liu, M. Sorrells, M. Heun, J.D. Franckowiak, D. Hoffmann, R. Skadsen & B.J. Steffenson, 1993. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor. Appl. Genet. 86: 705–712.
Knudsen, J.C.N., H.H. Dalsgaard & J.H. Jørgensen, 1987. Partial resistance to powdery mildew. In: S. Yasuda & T. Konishi (Eds.), Barley Genetics V, pp. 645–666. Okayama University Press, Japan.
Kølster, P. & O. Stølen, 1987. Barley isolines with genes for resistance to Erysiphe graminis f.sp. hordei in the recurrent parent Siri. Plant Breeding 98: 79–82.
Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–178.
Mather, D.E., N.A. Tinker, D.E. LaBerge, M. Edney, B.L. Jones, B.G. Rossnagel, W.G. Legge, K.G. Briggs, R.B. Irvine, D.E. Falk & K.J. Kasha, 1997. Regions of the genome that affect grain and malt quality in a North American two-row barley cross. Crop Sci. 37: 544–554.
Moseman, J.G., 1972. Isogenic barley lines for reaction to Erysiphe graminis f.sp. hordei. Crop Sci. 12: 681–682.
Saghai Maroof, M.A., Q. Zhang & R.M. Biyashev, 1994. Molecular marker analysis of powdery mildew resistance in barley. Theor. Appl. Genet. 88: 733–740.
Spaner, D., L.P. Shugar, T.M. Choo, I. Falak, K.G. Briggs, W.G. Legge, D.E. Falk, S.E. Ullrich, N.A. Tinker, B.J. Steffenson & D.E. Mather, 1998. Mapping of disease resistance loci in barley on the basis of naturally occurring symptoms. Crop Science 38: 843–850.
Thomas, W.T.B., W. Powell, R. Waugh, K.J. Chalmers, U.M. Barua, P. Jack, V. Lea, B.P. Forster, J.S. Swanston, R.P. Ellis, P.R. Hanson & R.C.M. Lance, 1995. Detection of quantitative trait loci for agronomic, yield, grain and disease characters in spring barley (Hordeum vulgare L.). Theor. Appl. Genet. 91: 1037–1047.
Tinker, N.A. & D.E. Mather, 1995a. Methods for QTL analysis with progeny replicated in multiple environments. J. Quantitative Trait Loci 1:1 (http://probe.nalusda.gov:8000/otherdocs/jqtl/1995–01/jqtl15.html).
Tinker, N.A. & D.E. Mather, 1995b. MQTL: software for simplified composite interval mapping of QTL in multiple environments. J. Quantitative Trait Loci 1: 2. (http://probe.nalusda.gov:8000/otherdocs/jqtl/995–02/jqtl16r2.html).
Tinker, N.A., D.E. Mather, B.G. Rossnagel, K.J. Kasha, A. Kleinhofs, P.M. Hayes, D.E. Falk, T. Ferguson, L.P. Shugar, W.G. Legge, R.B. Irvine, T.M. Choo, K.G. Briggs, S.E. Ullrich, J.D. Franckowiak, T.K. Blake, R.J. Graf, S.M. Dofing, M.A. Saghai Maroof, G.J. Scoles, D. Hoffman, L.S. Dahleen, A. Kilian, F. Chen, R.M. Biyashev, D.A. Kudrna & B.J. Steffenson, 1996. Regions of the genome that affect agronomic performance in two-row barley. Crop Sci. 36: 1053–1062.
Wiberg, A., 1974. Genetical studies of spontaneous sources of resistance to powdery mildew in barley. Hereditas 77: 89–148.
Wolfe, M.S., U. Brandle, B. Koller, E. Limpert, J.M. McDermott, K. Muller & D. Schaffner, 1992. Barley mildew in Europe: population biology and host resistance. Euphytica 63: 125–139.
Zadoks, J.C., T.T. Chang & C.F. Konzak, 1974. A decimal code for the growth stages of cereals. Weed Res. 14: 415–421.
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Falak, I., Falk, D., Tinker, N. et al. Resistance to powdery mildew in a doubled haploid barley population and its association with marker loci. Euphytica 107, 185–192 (1999). https://doi.org/10.1023/A:1003696330951
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DOI: https://doi.org/10.1023/A:1003696330951