Abstract
Ceratitis capitata is one of the most important pest species in the tropical and temperate regions of the world, however, genetic knowledge of this species is still very limited. In the present study, we have attempted for the first time an analysis of the genetic variability in seven natural populations of C. capitata by means of abundant soluble proteins, combining high resolution techniques such as polyacrylamide gel electrophoresis and silver staining methods. A total of 66 polypeptides have been analyzed. The results show the existence of a decreasing trend in the ‘levels’ of polymorphism from the southern areas to the northern that is correlated with the latitude of the sampling areas, most probably due to the colonization process of the Iberian Peninsula. On the other hand, and with relation to the ‘pattern’ of the variability, a geographic differentiation (not strictly latitudinal) is detected when an UPGMA clustering method was applied to the data. Moreover, principal component anaysis has revealed that a part of this differentiation could be explained in relation with environmental factors such as annual rainfall and minimum temperature in winter months. This is suggesting that selection (added to the historical process) could be playing an important role in the process of geographic differentiation, ‘shaping’ the pattern of that variability. No host-related differentiation has been observed in these samples.
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Aquadro C.F. & J.C. Avise, 1981. Genetic divergence between rodent species assessed by using two-dimensional gel electrophoresis. Proc. Natl. Acad. Asci. U.S.A. 78: 3784-3788.
Avise, J.C., 1994. Molecular markers, natural history and evolution. Chapman and Hall, Inc.
Baruffi, L., G. Damiani, C.R. Guglielmino, C. Bandi, A.E. Malacrida & G. Gasperi, 1995. Polymorphism within and between populations of Ceratitis capitata: comparison between RAPD and multilocus enzyme electrophoresis data. Heredity 74: 425-437.
Chakraborty, R. & M. Nei, 1976. Hidden genetic variability within electromorphs in finite populations. Genetics 84: 385-393.
De Breme, F., 1842. Note sur le genre Ceratitis de M. Macheay (Diptera). Ann. Ent. Soc. Fr. 11: 183-190.
Felsenstein, J., 1993. PHYLIP: Phylogenetic Inference Package. Version 3.5c, University of Washington, Seattle.
Fletcher, B.S., 1989. Life history strategies of tephritid fruit flies, pp. 195-208. In Fruit Flies: their Biology, Natural Enemies and Control, Vol. 3B, Edited by A.S. Robinson and G. Hooper. Elsevier, Amsterdam.
Garrido, A. & J.J. Ventura, 1993. Ceratitis capitata Wied (Mosca Mediterranea) pp. 103-108. In: Plagas de los Citricos. Ministerio de Agricultura Pesca y Alimentacion. Madrid.
Gasperi, G., C.R. Guglielmino, A.R. Malacrida & R. Milani, 1991. Genetic variability and gene flow in geographical populations of Ceratitis capitata (Wied.) (medfly). Heredity 67: 347-356.
Gasperi, G., A.R. Malacrida & R. Milani, 1987. Protein variability and population genetics pp. 149-157. In: Fruit Flies, Edited by A.P. Economopoulos. Elsevier. Amsterdam.
Hageman, C. & D. Fahselt, 1984. Intraspecific variability of isozymes of lichen Umbilicaria mammulata. Can. J. Bot. 62: 617-623.
Hagen, K.S., W.W. William & R.L. Tassan, 1981. Mediterranean fruit fly: The worst may be yet to come. California Agriculture 35: 5-7.
Haymer, D.S. & D.O. McInnis, 1994. Resolution of populations of the Mediterranean fruit fly at the DNA level using random primers for the polymerase chain reaction. Genome 35: 244-248.
Jaccard, P., 1908. Nouvelles recherches sur la distribution florale. Bull. Soc. Vauloise Sci. Nat. 44: 223-270.
Laemmli, V.K., 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227: 680-685.
Loukas, M., 1989. Population genetic studies of fruit flies of economic importance, specially medfly and olive fruit fly, using electrophoretic methods pp. 69-102. In: Electrophoretic Studies on Agricultural Pests, Edited by H.D. Loxdale and J. den Hollander. Syst. Ass. Special Vol. 39. Clarendon Press. Oxford.
Malacrida, A.R., C.R. Guglielmino, G. Gasperi, L. Baruffi & R. Milani, 1992. Spatial and temporal differentiation in colonizing populations of Ceratitis capitata. Heredity 69: 101-111.
Merril, C.R., M.L. Dunan & D. Goldman, 1981. A rapid sensitive silver stain for polypeptides in polyacrylamide gels. Anal. Biochem. 110: 201-207.
Mintzas, A.C. & M.D. Rina, 1986. Isolation and characterization of three major larval serum proteins of the Mediterranean fruit fly Ceratitis capitata (Diptera). Insect. Biochem. 16: 825-834.
McPheron, B.A., G.E. Gasparich, H.Y. Han, G.J. Steck & W.S. Sheppard, 1994. Mitochondrial DNA restriction map for the Mediterranean fruit fly, Ceratitis capitata. Biochem. Genet. 32: 25-33.
Nei, M., T. Maruyama & R. Chakraborty, 1975. The bottleneck effect and genetic variability in populations. Evolution 29: 1-10.
Ramagopal, S., 1990. Protein polymorphism in sugarcane revealed by two-dimensional gel analysis. Theor. Appl. Genet. 79: 297- 304.
Reyes, A., 1995. Analisis de la variabilidad genetica en problaciones españolas de Ceratitis capitata Wied. mediante la utilizacion de marcadores moleculares. Ph. D. Thesis Universidad Complutense de Madrid. pp. 202.
Reyes, A. & M.D. Ochando, 1994. A study of gene-enzyme variability in three Spanish populations of Ceratitis capitata. IOBC/WPRS Bull 17: 151-160.
Reyes, A. & M.D. Ochando, 1998.The use of molecular markers for detecting the geographical origin of Ceratitis capitata (Diptera: Tephritidae) populations. Ann. Ent. Soc. Am. 91, 2: 222-227.
Reyes, A., C. Callejas, P. Roda & M.D. Ochando, 1996. Caracterizacion genetica en Ceratitis capitata asociada a fruto hospedador. II. Analisis mediante RAPD-PCR. Bol. San. Veg. Plagas 22: 361-371.
Robinson, A.S., & G. Hooper G (Eds), 1989. Fruit flies: Their Biology, Natural Enemies and Control. Vol 3A and 3B. Elsevier. Amsterdam.
Roda, P., C. Callejas, A. Reyes & M.D. Ochando, 1996. Caracterizacion genetica en Ceratitis capitata asociada a fruto hospedador. I Analisis isoenzimatico. Bol. San. Veg. Plagas 22: 71-78.
Rohlf, J., 1990. Numerical taxonomy and multivariate analysis system. NTSYS-pc. Dept Ecol Evol. New York.
Sheppard, W.S., G.J. Steck & B.A. McPheron, 1992. Geographic populations of the medfly may be differentiated by mitochondrial DNA variation. Experientia 48: 1010-1013.
Singh, R.S. & A.D. Long, 1992. Geographic variation in Drosophila: From molecules to morphology and back. Trends Ecol. Evol. 7: 340-345.
Sneath, P.H.A. & R.R. Sokal, 1973. Numerical taxonomy. Freeman. San Francisco.
Zen, L.W., & R.S.A. Singh, 1993. A combined classical genetic and high resolution two-dimensional electrophoretic approach to the assessment of the number of genes affecting hybrid male sterility in Drosophila simulans and Drosophila sechellia. Genetics 135: 135-147.
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Reyes, A., Ochando, M.D. Genetic differentiation in Spanish populations of Ceratitis capitata as revealed by abundant soluble protein analysis. Genetica 104, 59–66 (1998). https://doi.org/10.1023/A:1003483913805
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DOI: https://doi.org/10.1023/A:1003483913805