Abstract
Brassica napus somatic hybrids with low linolenic acid (18:3) content in their seed oil have been produced using fusion partners screened for low 18:3. One somatic hybrid contained only 3.5% 18:3, a level significantly below the mid-parental mean. The low level of 18:3 proved stable in the R1 generation. Oil content of the lowest 18:3 selection increased from the mid-parental mean (29.3%) in the R0 generation to 36% in a R1 field bulk. The R1 field population also showed some resistance to shattering.
Similar content being viewed by others
References
Arus, P. & T.J. Orton, 1983. Inheritance and linkage relationships of isozyme loci in Brassica oleracea. J Hered 74: 405–412.
Bierenbaum, M.L., R.P. Reichstein, T.R. Watkins, W.P. Maginnis & M. Geller, 1991. Effects of canola oil on serum lipids in humans. J Am Coll Nutr 10: 228–233.
Chen, B.Y. & W.K. Heneen, 1989. Fatty acid composition of resynthesized Brassica napus L., B. campestris L. and B. alboglabra Bailey with special reference to the inheritance of erucic acid content. Heredity 63: 309–314.
Diepenbrock, W. & R.F. Wilson, 1987. Genetic regulation of linolenic acid concentration in rapeseed. Crop Sci 27: 75–77.
Downey, R.K., 1988. From rapeseed to canola and beyond. In: L.L. Hardman & L. Waters (Eds) Strategies of alternative crop development: case histories, pp. 17–31. Center for alternative plant and animal products. Univ of Minnesota, St. Paul.
Downey, R.K. & G. Robbelen, 1989. Brassica species. In: G. Robbelen, R.K. Downey & A. Ashri (Eds) Oil Crops of the World, pp. 339–362. McGraw-Hill Publishing Co., New York.
Dupont, J., P.J. White, K.M. Johnston, H.A. Heggtveit, B.E. McDonald, S.M. Grundy & A. Bonanome, 1989. Food safety and health effects of canola oil. J Am Coll Nutr 8: 360–375.
Fahleson, J., I. Eriksson, Landgren, S. Stymne & K. Glimelius, 1994. Intertribal somatic hybrids between Brassica napus and Thlaspi perfoliatum with high content of T. perfoliatum-specific nervonic acid. Theor Appl Genet 87: 795–804.
Glimelius, K., J. Fahlesson, C. Sjodin, E. Sundberg & M. Djupsjobacka, 1986. Somatic hybridization and cybridization as potential methods for widening of the gene-pools of crops with Brassicaceae and Solanaceae. In: Horn, Jensen, Odenbach & Schieder (Eds) Genetic manipulation in plant breeding, pp. 663–682. Walter de Gruyter & Co., Berlin.
Heath, D.W. & E.D. Earle, 1995. Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters. Theor Appl Genet 91: 1129–1136.
Heather, D.W., 1993. Fatty acid modification and other improvements in rapeseed (Brassica napus L.) through sexual and somatic hybridization. PhD Diss Cornell University, Ithaca, NY.
Jourdan, P.S., E.D. Earle & M.A. Mutschler, 1989. Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris. Theor Appl Genet 78: 445–455.
Mahler, K.A. & D.L. Auld, 1989. Fatty acid composition of 2100 accessions of Brassica. Idaho Agric Exp Stn Misc Ser 125.
Ozminkowski, R.H. Jr., 1992. Comparison of sexual and somatic interspecific hybridization in Brassica. PhD Dissertation, Ohio State Univ., Columbus, OH.
Pelletier, G., C. Primard, F. Vedel, P. Chetrit, R. Remy, P. Rousselle & M. Renard, 1983. Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191: 244–250.
Prevot, A., J.L. Perrin, G. LaClaverie, P. Auge & J.L. Coustille, 1990. A new variety of low linolenic rapeseed oil characteristics and room odor tests. J Am Oil Chem Soc 67: 161–164.
Roy, N.N. & A.W. Tarr, 1986. Development of near-zero linolenic acid (18:3) lines of rapeseed (Brassica napus). Z Pflanzenzücht 96: 218–223.
Roy, N.N. & A.W. Tarr, 1987. Prospects for the development of rapeseed (Brassica napus L.) with improved linoleic and linolenic acid content. Plant Breeding 98: 89–96.
Scarth, R., P.B.E. McVetty, S.R. Rimmer & B.R. Stefansson, 1988. Stellar low linolenic-high linoleic acid summer rape. Can J Plant Sci 68: 509–512.
Schenck, H.R. & G. Robbelen, 1982. Somatic hybrids by fusion of protoplasts from Brassica oleracea and B. campestris. Z Pflanzenzüchtg 89: 278–288.
Taguchi, T. & T. Kameya, 1986. Production of somatic hybrid plants between cabbage and chinese cabbage through protoplast fusion. Jpn J Breed 36: 185–189.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Heath, D.W., Earle, E.D. Synthesis of low linolenic acid rapeseed (Brassica napus L.) through protoplast fusion. Euphytica 93, 339–343 (1997). https://doi.org/10.1023/A:1002948600224
Issue Date:
DOI: https://doi.org/10.1023/A:1002948600224