Abstract
Heat shock proteins (HSPs) ranging in molecular masses from 14 to 110 kDa were induced in embryonic axes of germinating Cajanus cajan (L.) Millspaugh seeds after exposure to 40 °C for 1 or 2 h. At 45 °C, there was a marked decline in synthesis of HSPs. A close relationship was observed between HSPs induced and the growth of the germinating seeds. Pretreatment of germinating seeds at 40 °C for 1 h or 45 °C for 10 min followed by incubation at 28 °C for 3 h led to considerable thermotolerance (45 °C, 2 h) and the recovery of protein synthesis.
Similar content being viewed by others
References
Ashburner, M., Bonner, J.: The induction of gene activity in Drosophila by heat shock.-Cell 17: 241–254, 1979.
Barnett, T., Altschuler, M., McDaniel, C.N., Mascarenhas, J.P.: Heat shock induced proteins in plant cells.-Dev. Genet. 1: 331–340, 1980.
Baszczynski, C.L., Walden, D.B., Atkinson, B.G.: Regulation of gene expression in corn (Zea mays L.) by heat shock.-Can. J. Biochem. 60: 569–579, 1982.
Black, A.R., Subjeck, J.R.: Correlation in the recovery of normal protein synthesis and the development of thermotolerance.-J. Cell Biol. 103: 188a, 1986.
Black, A.R., Subjeck, J.R.: Involvement of rRNA synthesis in enhanced survival and recovery of protein synthesis seen in thermotolerance.-J. Cell Physiol. 138: 439–449, 1989.
Chen, Y.M., Kamisaka, S., Masuda, Y.: Enhancing effects of heat shock and gibberellic acid on the thermotolerance in etiolated Vigna radiata. I. Physiological aspects on thermotolerance.-Physiol. Plant. 66: 595–601, 1986.
Cooper, P., Ho, T.H.D.: Heat shock proteins in maize.-Plant Physiol. 71: 215–222, 1983.
Feierabend, J., Reichhardt, U.S.: Biochemical differentiation of plastids and other organelles in rye leaves with a high-temperature induced deficiency of plastid ribosomes.-Planta 129: 133–145, 1976.
Ferguson, I.B., Lurie, S., Bowen, J.H.: Protein synthesis and breakdown during heat shock of cultured pear (Pyrus communis L.) cells.-Plant Physiol. 104: 1429–1437, 1994.
Howarth, C.J.: Heat shock proteins in Sorghum bicolor and Pennisetum americanum I. Genotypic and developmental variation during seed germination.-Plant Cell Environ. 12: 471–477, 1989.
Howarth, C.J., Ougham, H.J.: Gene expression under temperature stress.-New Phytol. 125: 1–26, 1993.
Key, J.L., Lin, C.Y., Chen, Y.M.: Heat shock proteins of higher plants.-Proc. nat. Acad. Sci. USA 78: 3526–3530, 1981.
Kimple, J.A., Key, J.L.: Heat shock in plants.-Trends biochem. Sci. 10: 353–357, 1985.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the heat of bacteriophage T4.-Nature 227: 680–685, 1970.
Lin, C., Roberts, J.K., Key, J.L.: Acquisition of thermotolerance in soybean seedlings.-Plant Physiol. 74: 152–160, 1984.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.L.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.
Mans, R.J., Novelli, G.D.: Measurement of the incorporation of radioactive amino acids into protein by a filter paper disc method.-Arch. Biochem. Biophys. 94: 48–53, 1961.
Mizzen, L.A., Welch, W.J.: Characterization of the thermotolerant cell. I. Effect on protein synthesis activity and regulation of heat shock protein 70 expression.-J. Cell Biol. 106: 1105–1116, 1988.
Moisyadi, S., Harrington, H.M.: Characterization of the heat shock response in cultured sugarcane cells.-Plant Physiol. 90: 1156–1162, 1989.
Nover, L., Scharf, K.D., Neumann, D.: Formation of cytoplasmic heat shock granuels in tomato cell cultures and leaves.-Mol. cell. Biol. 3: 1648–1655, 1983.
Ougham, H.J., Stoddart, J.L.: Development of a laboratory screening technique based on embryo protein synthesis for the assessment of high-temperature susceptibility during germination of sorghum bicolor.-Exp. Agr. 21: 343–355, 1985.
Ougham, H.J., Stoddart, J.L.: Synthesis of heat shock protein and acquisition of thermotolerance in high temperature tolerant and high temperature susceptible lines of Sorghum.-Plant Sci. 44: 163–167, 1986.
Pelham, H.R.B.: Activation of heat shock genes in eukaryotes.-Trends Genet 1: 31–35, 1985.
Petersen, N.S., Mitchel, H.K.: Recovery of protein synthesis after heat shock: Prior heat treatment affects the ability of cells to translate mRNA.-Proc. nat. Acad. Sci. USA 78: 1708–1711, 1981.
Riley, G.J.P.: Effects of high temperature on the germination of maize (Zea mays L.).-Planta 151: 68–74, 1981.
Rochester, D.E., Winer, J.A., Shah, D.M.: The structure and expression of maize genes encoding the major heat shock protein, HSP 70.-Eur. mol. Biol. Org. J. 5: 451–458, 1986.
Rose, D.W., Welch, W.J., Krainer, G., Hardesty, B.: Possible involvement of the 90 kDa heat shock protein in the regulation of protein synthesis.-J. biol. Chem. 264: 6239–6244, 1989.
Schlesinger, M.J., Ashburner, M., Tissieres, A.: Heat Shock from Bacteria to Man.-Cold Spring Harbour, New York 1982.
Sivaramakrishnan, S., Patel, V.Z., Soman, P.: Heat shock proteins of sorghum (Sorghum bicolor (L.) Moench) and pearl millet (Pennisetum glaucum (L.) R.Br.) cultivars with differing heat tolerance at seedling establishment stage.-J. exp. Bot. 41: 249–254, 1990.
Somers, D.J., Cummins, W.R., Filion, W.G.: Characterization of the heat shock response in spinach (Spinacea oleracea L.).-Biochem. Cell Biol. 67: 113–120, 1989.
Vierling, E.: The roles of heat shock proteins in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 579–620, 1991.
Welch, W.J., Suhan, J.P.: Morphological studies of the mammalian stress response. Characterization of changes in cytoplasmic organelles, cytoskeleton and nucleoli, and appearance of intranuclear action filaments in rat fibroblasts after heat shock.-J. Cell Biol. 101: 1198–1211, 1985.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sri Devi, V., Satyanarayana, N. & Madhava Rao, K. Induction of Heat Shock Proteins and Acquisition of Thermotolerance in Germinating Pigeonpea Seeds. Biologia Plantarum 42, 589–597 (1999). https://doi.org/10.1023/A:1002635602823
Issue Date:
DOI: https://doi.org/10.1023/A:1002635602823