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Changes in heat-shock protein synthesis and thermotolerance of Arabodopsis thaliana seedlings resulting from Hsp90 inhibition by geldanamycin

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Abstract

The influence of geldanamycin (GA), a specific inhibitor of heat-shock protein Hsp90, on the synthesis of Hsp70 and Hsp90 and thermotolerance of Arabidopsis thaliana seedlings has been studied. Incubation of seedlings with GA under normal conditions induced synthesis of these stress proteins. Treatment of seeds with the Hsp90 inhibitor resulted in elevated constitutive levels of Hsp70 and Hsp90 in seedlings, as well as increased induction of their synthesis under heat shock. The GA effect increased with its concentration. Hsp up-regulation promoted thermotolerance of seedlings. The findings suggest autoregulation of heatshock protein synthesis and regulation of plant tolerance by Hsp90.

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Abbreviations

GA:

geldanamycin

DMSO:

dimethylsulfoxide

HSE:

heat-shock element

Hsp:

heat-shock protein

Hsf:

heat-shock factor

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Authors and Affiliations

  1. Institute of Botany, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    L. Ye. Kozeko

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  1. L. Ye. Kozeko
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Correspondence to L. Ye. Kozeko.

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Original Russian Text © L.Ye. Kozeko, 2014, published in Tsitologiya, 2014, Vol. 56, No. 6, pp. 419–426.

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Kozeko, L.Y. Changes in heat-shock protein synthesis and thermotolerance of Arabodopsis thaliana seedlings resulting from Hsp90 inhibition by geldanamycin. Cell Tiss. Biol. 8, 416–422 (2014). https://doi.org/10.1134/S1990519X14050046

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  • DOI: https://doi.org/10.1134/S1990519X14050046

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