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Identification and Mapping of a T-DNA Induced Flower Mutation in Arabidopsis Thaliana

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Biologia Plantarum

Abstract

Collection of the T-DNA tagged lines of Arabidopsis thaliana have been created by Agrobacterium-mediated root transformation. Transgenic lines produced by this method have been screened for morphogenic mutations. A flower mutation with increased number of stamens and carpels (scaf1) was identified. This mutation has similar but weaker phenotype than the known mutant superman. Two mapping procedures, with visible and molecular markers, were used to locate scaf1 flower mutation. Genetic analysis showed that this mutation is located on chromosome 3 near gl1 gene. It is probably one of the SUPERMAN epigenetic alleles.

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Authors and Affiliations

  1. University of South Bohemia, Faculty of Biological Sciences, Branišovská 31, 370 05, České Budějovice, Czech Republic

    T. Kocábek

  2. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic

    S. Rakouský & M. Ondřej

  3. Faculty of Sciences, Department of Genetics and Molecular Biology, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic

    J. Řepková & J. Relichová

Authors
  1. T. Kocábek
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  2. S. Rakouský
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  3. M. Ondřej
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  4. J. Řepková
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  5. J. Relichová
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Kocábek, T., Rakouský, S., Ondřej, M. et al. Identification and Mapping of a T-DNA Induced Flower Mutation in Arabidopsis Thaliana. Biologia Plantarum 42, 349–359 (1999). https://doi.org/10.1023/A:1002405016874

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  • DOI: https://doi.org/10.1023/A:1002405016874

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