Abstract
High frequency shoot regeneration from cotyledons excised from 4-d-old seedlings of Brassica campestris L. cv. M 27 and Brassica juncea (L.) Czern. cv. Pusabold was achieved on Murashige and Skoog's (MS) medium supplemented with 1.0 mg dm-3 N6-benzyladenine (BA) and 3 % (m/v) saccharose. Rooting occurred simultaneously with shoot formation on the medium containing 1.0 mg dm-3 BA and 0.5 mg dm-3 1-naphthaleneacetic acid. Cultures of cotyledon, cotyledon derived non-differentiating calli and differentiated calli with shoots and/or roots were analysed at different intervals for isozyme patterns of esterase and peroxidase. With the BA-induced development of shoot and/or root from non-differentiating callus, some conspicuous isozymes appeared which indicates the involvement of these isozymes in root and shoot development rather than in induction of morphogenesis in callus.
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Samantaray, S., Rout, G. & Das, P. Changes in Isozyme Patterns During in Vitro Regeneration from Cotyledon Explants of Brassica Species. Biologia Plantarum 42, 169–176 (1999). https://doi.org/10.1023/A:1002196231904
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DOI: https://doi.org/10.1023/A:1002196231904