Abstract
Potato (Solanum tuberosum L. cv. Bintje) was transformed with a cDNA clone encoding an osmotin-like protein. Transgenic and non-transgenic in vitro plants were subjected to NaCl for 3 weeks. The shoot and root development was slightly affected by salinity indicating that the salt condition used was a mild stress. The endogenous proline content of the osmotin-like transformed clone only raised slightly as compared to the non-transformed genotype, where a marked increase in proline content could be observed as a result to salt stress. These data provide evidence for the involvement of osmotin-like proteins in the mechanisms of salt tolerance in potato plants.
Similar content being viewed by others
References
Abad, L.R., Paino d'Urzo, M., Liu, D., Narasimham, M.L., Reuveni, M., Zhu, J.K., Niu, X., Singh, N.K., Hasegawa, P.M., Bressan, R.A.: Antifungal activity of tobacco osmotin has specificity and involves plasma membrane permeabilization.-Plant Sci. 118: 11–23, 1996.
Bandurski, R.S., Cohen, J.D., Slovin, J.P., Reinecke, D.M.: Auxin biosynthesis and metabolism.-In: Davies, P.J. (ed.). Plant Hormones. Pp. 39–65. Kluwer Academic Publishers, Dordrecht-Boston-London 1995.
Barker, H., Reavy, B., Kumar, A., Webster, K.D., Mayo, M.A.: Restricted virus multiplication in potatoes transformed with the coat protein gene of potato leafroll luteovirus: similarities with a type of host gene-mediated resistance.-Ann. appl. Biol. 120: 55–64, 1992.
Barker, H., Webster, K.D., Jolly, C.A., Reavy, B., Kumar, A., Mayo, M.A.: Enhancement of resistance to potato leafroll virus multiplication in potato by combining the effects of host genes and transgenes.-Mol. Plant-Microbe Interact. 7: 528–530, 1994.
Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water stress studies.-Plant Soil 39: 205–207, 1973.
Bevan, M.W.: Binary Agrobacterium vectors for plant transformation. Nucl. Acids Res. 12: 8711–8721, 1984.
Capelli, N., Diogon, T., Greppin, H., Simon, P.: Isolation and characterization of a cDNA clone encoding an osmotin-like protein from Arabidopsis thaliana.-Gene 191: 51–56, 1997.
Chang, P.F.L., Cheah, K.T., Narasimhan, M.L., Hasegawa, P.M., Bressan, R.A.: Osmotin gene expression is controlled by elicitor synergism.-Physiol. Plant. 95: 620–626, 1995.
Cleland, R.: The role of hormones in wall loosening and plant growth.-Aust. J. Plant Physiol. 13: 93–103, 1986.
Delauney, A.J., Verma, D.P.S.: Proline biosynthesis and osmoregulation in plants.-Plant J. 4: 215–223, 1993.
Edwards, K., Johnstone, C., Thompson, C.: A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.-Nucl. Acids Res. 19: 1349, 1991.
Evers, D., Schmit, C., Mailliet, Y., Hausman, J.F.: Growth characteristics and biochemical changes of poplar shoots in vitro under sodium chloride stress.-J. Plant Physiol. 151: 748–753, 1997.
Evers, D., Hemmer, K., Hausman, J.F.: Salt stress induced biometric and physiological changes in Solanum tuberosum L. cv Bintje grown in vitro.-Acta Physiol. Plant. 20: 3–7, 1998.
Gadallah, M.A.A.: Abscisic acid, temperature and salinity interactions on growth and some mineral elements in Carthamus plants.-Plant Growth Regul. 20: 225–236, 1996.
Gaspar, T., Kevers, C., Hausman, J.F., Berthon, J.Y., Ripetti, V.: Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots.-Agronomie 12: 757–765, 1992.
Gaspar, T., Kevers, C., Hausman, J.F., Ripetti, V.: Peroxidase activity and endogenous free auxin during adventitious root formation.-In: Lumsden, P.J., Nicholas, J.R., Davies, W.J. (ed.): Physiology, Growth and Development of Plants in Culture. Pp. 289–298. Kluwer Academic Publishers Dordrecht-Boston-London 1994.
Hervieu, F., LeDily, F., Huault, C., Billard, J.P.: Contribution of ornithine aminotransferase to proline accumulation in NaCl-treated radish cotyledons.-Plant Cell Environ. 18: 205–210, 1995.
Kononowicz, A.K., Nelson, D.E., Singh, N.K., Hasegawa, P.M., Bressan, R.A.: Regulation of the osmotin gene promoter.-Plant Cell 4: 513–524, 1992.
LaRosa, P.C., Chen, Z., Nelson, D.E., Singh, N.K., Hasegawa, P.M., Bressan, R.A.: Osmotin gene expression is posttranscriptionally regulated.-Plant Physiol. 100: 409–415, 1992.
Leshem, Y.Y., Kuiper, P.J.C.: Is there a GAS (general adaptation syndrome) response to various types of environmental stress?-Biol. Plant. 38: 1–18, 1996.
Lichtenthaler, H.K.: Vegetation stress: an introduction to the stress concept in plants.-J. Plant Physiol. 148: 4–14, 1996.
Lin, C.C., Kao, C.H.: Proline accumulation is associated with inhibition of rice seedling root growth caused by NaCl.-Plant Sci. 114: 121–128, 1996.
Liu, D., Raghothama, K.G., Hasegawa, P.M., Bressan, R.A.: Osmotin overexpression in potato delays development of disease symptoms.-Proc. nat. Acad. Sci. USA 91: 1888–1892, 1994.
Liu, D., Rhodes, D., Paino D'Urzo, M., Xu, Y., Narasimhan, M. L., Hasegawa, P.M., Bressan, R.A., Abad, L.: In vivo and in vitro activity of truncated osmotin that is secreted into the extracellular matrix.-Plant Sci. 121: 123–131, 1996.
Lutts, S., Kinet, J.M., Bouharmont, J.: Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance.-Plant Growth Regul. 19: 207–218, 1996.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473–497, 1962.
Nelson, D.E., Raghothama, K.G., Singh, N.K., Hasegawa, P.M., Bressan, R.A.: Analysis of structure and transcriptional activation of an osmotin gene.-Plant mol. Biol. 19: 577–588, 1992.
Rhodes, D.: Metabolic responses to stress.-In: Stumpf, P.K., Conn, E.E. (ed.): The Biochemistry of Plants: A Comprehensive Treatise. Vol. 12. Physiology of Metabolism. Pp. 201–241. Academic Press, San Diego 1987.
Rhodes, D., Handa, S., Bressan, R.A.: Metabolic changes associated with adaptation of plant cells to water stress.-Plant Physiol. 82: 890–903, 1986.
Singh, N.K., Handa, A.K., Hasegawa, P.M., Bressan, R.A.: Proteins associated with adaptation of cultured tobacco cells to NaCl.-Plant Physiol. 79: 126–137, 1985.
Singh, N.K., Bracker, C.A., Hasegawa, P.M., Handa, A.K., Buckel, S., Hermodson, M.A., Pfankock, E., Regnier, F.E., Bressan, R.A.: Characterization of osmotin: a thaumatin-like protein associated with osmotic adaptation in plant cells.-Plant Physiol. 85: 529–536, 1987a.
Singh, N.K., LaRosa, P.C., Handa, A.K., Hasegawa, P.M., Bressan, R.A.: Hormonal regulation of protein synthesis associated with salt tolerance in plant cells.-Proc. nat. Acad. Sci. USA 84: 739–743, 1987b.
Singh, N.K., Nelson, D.E., Kuhn, D., Hasegawa, P.M., Bressan, R.A.: Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential.-Plant Physiol. 90: 1096–1101, 1989.
Vartanian, N.: Mutants as tools to understand cellular and molecular drought tolerance mechanisms.-Plant Growth Regul. 20: 125–134, 1996.
Venekamp, J.H.: Regulation of cytosol acidity in plants under conditions of drought.-Physiol. Plant. 76: 112–117, 1989.
Zhu, B., Chen, T.H.H., Li, P.H.: Expression of an ABA responsive osmotin-like gene during the induction of freezing tolerance in Solanum commersonii.-Plant mol. Biol. 21: 729–735, 1993.
Zhu, B., Chen, T.H.H., Li, P.H.: Activation of two osmotin-like protein genes by abiotic stimuli and fungal pathogen in transgenic potato plants.-Plant Physiol. 108: 929–937, 1995.
Zhu, B., Chen, T.H.H., Li, P.H.: Analysis of late-blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin-like protein.-Plant 198: 70–77, 1996.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evers, D., Overney, S., Simon, P. et al. Salt Tolerance of Solanum tuberosum L. Overexpressing an Heterologous Osmotin-like Protein. Biologia Plantarum 42, 105–112 (1999). https://doi.org/10.1023/A:1002131812340
Issue Date:
DOI: https://doi.org/10.1023/A:1002131812340