Abstract
A Nicotiana tabacum gene encoding the basic PR-like protein osmotin was isolated and characterized. The gene is derived from the N. sylvestris parent of N. tabacum. In cell suspension cultures of tobacco, the osmotin gene was shown to be transcriptionally activated by treatment with ABA.
Transcriptional activation of the osmotin promoter was further investigated in transformed plants carrying copies of a fusion of the cloned promoter to the β-glucuronidase reporter gene. In these plants, the osmotin promoter is transcriptionally activated by the hormones ABA and ethylene. The sensitivity of the osmotin promoter to ABA applied exogenously decreased with age in both roots and shoots of young seedlings. NaCl shock also activated the promoter in plant tissues. The osmotin promoter is much more active in root tissues than in shoot tissues.
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References
Broglie KE, Biddle P, Cressman R, Broglie R: Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco. Plant Cell 1: 599–607 (1989).
Dyer WE, Henstrand JM, Handa AK, Herrmann KM: Wounding induces the first enzyme of the shikimate pathway in Solanaceae. Proc Natl Acad Sci USA 86: 7370–7373 (1989).
Finkelstein RR, Somerville CR: Three classes of abscisic acid (ABA)-insensitive mutations of Arabidopsis define genes that control overlapping subsets of ABA responses. Plant Physiol 94: 1172–1179 (1990).
Guiltinan MJ, Marcotte WR, Quatrano RS: A plant leucine zipper protein that recognizes an abscisic acid response element. Science 250: 267–271 (1990).
Hasegawa PM, Bressan RA, Handa AK. Growth characteristics of NaCl-selected and non-selected cells of Nicotiana tabacum L. Plant Cell Physiol 21: 1347–1355 (1980).
Hatzopoulos P, Franz G, Choy L, Sung RZ: Interaction of nuclear factors with upstream sequences of a lipid body membrane protein from carrot. Plant Cell 2: 457–467 (1990).
Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
LaRosa PC, Chen Z, Nelson DE, Singh NK, Hasegawa PM, Bressan RA: Osmotin gene expression is post-transcriptionally regulated. Plant Physiol (in press) (1992).
Lawton MA, Lamb CJ: Transcriptional activation of plant defense genes by fungal elicitor, wounding and infection. Mol Cell Biol 7: 335–341 (1987).
Marcotte WR, Bayley CC, Quatrano RS: Regulation of a wheat promoter by abscisic acid in rice protoplasts. Nature 335: 454–457 (1988).
Marcotte WR, Russell SH, Quatrano RS: Abscisic acid-responsive sequences from the Em gene of wheat. Plant cell 1: 969–976 (1989).
Mundy J, Yamaguchi-Shinozaki K, Chua N-H: Nuclear proteins bind conserved elements in the abscisic acid-responsive promoter of a rice rab gene. Proc Natl Acad Sci USA 87: 1406–1410 (1990).
Neale AD, Wahleithner JA, Lund M, Bonnett HT, Kelly A, Meeks-Wagner DR, Peacock WJ, Dennis ES: Chitinase, B-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation. Plant Cell 2: 673–684 (1990).
Pena-Cortes H, Sanchez-Serrano JJ, Mertens R, Willmitzer L: Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato. Proc Natl Acad Sci USA 86: 9851–9855 (1990).
Richardson M, Valdes-Rodriguez S, Blanco-Labra A: A possible function for thaumatin and a TMV-induced protein suggested by homology to a maize inhibitor, Nature 327: 432–434 (1987).
Roberts WK, Seletrennikoff CP: Zeamatin, an antifungal protein from maize with membrane permeabilizing activity. J Gen Microbiol 136: 1771–1778 (1990).
Singh NK, Bracker CA, Hasegawa PM, Handa AK, Buckel S, Hermodson MA, Pfankock E, Regnier FE, Bressan RA: Characterization of osmotin. Plant Physiol 85: 529–536 (1987).
Singh NK, LaRosa PC, Handa AK, Hasegawa PM, Bressan RA: Hormonal regulation of protein synthesis associated with salt tolerance in plant cells. Proc Natl Acad Sci USA 84: 739–743 (1987).
Singh NK, Nelson DE, Kuhn D, Hasegawa PM, Bressan RA. Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential. Plant Physiol 90: 1096–1101 (1989).
Skriver K, Mundy J. Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2: 503–521 (1990).
Stintzi A, Heitz T, Kaufmann S, Legrand M, Fritig B: Identification of a basic pathogenesis protein of virus-infected tobacco as osmotin. Physiol Mol Plant Path 37, in press (1991).
vanKan JAL, van deRhee MD, Zuidema D, Cornelissen BJC, Bol JF: Structure of tobacco genes encoding thaumatin-like proteins. Plant Mol Biol 12: 153–155 (1989).
Vigers AJ, Roberts WKD, Seletrennikoff CP: A new family of plant antifungal protein. Mol Plant-Microbe Inter 4: 315–323 (1991).
Woloshuk C, Muelenhoff J, Sela-Buurlage M, van denElzen P, Cornelissen B. Pathogen-induced proteins with inhibitory activity toward Phytophthora infestans. Plant Cell 3: 619–628 (1991).
Yamaguchi-Shinozaki K, Mino M, Mundy J, Chua N-H: Analysis of an ABA-responsive rice gene promoter in transgenic tobacco. Plant Mol Biol 15: 905–912 (1990).
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Nelson, D.E., Raghothama, K.G., Singh, N.K. et al. Analysis of structure and transcriptional activation of an osmotin gene. Plant Mol Biol 19, 577–588 (1992). https://doi.org/10.1007/BF00026784
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DOI: https://doi.org/10.1007/BF00026784