Skip to main content
SpringerLink
Log in

Responses to chilling stress in French bean seedlings: antioxidant compounds

  • Published:
Biologia Plantarum

Abstract

French bean (Phaseolus vulgaris L. cv. Contender) plants at five developmental stages (4, 8, 12, 16 and 20 d after sowing) were exposed to one of three treatments: 1 - 25 °C (control), 2 - exposure to chilling at 10 °C only for 2 d prior to sampling, and 3 - long-term exposure to chilling at 10 °C. Short- and long-term chilling decreased plant growth. Higher concentrations of ascorbate and glutathione were found in the chilling-treated plants throughout the different period of growth in comparison with those in the control plants. The activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase increased in the chilling-treated seedlings while activities of catalase and peroxidase and of β-carotene content decreased in young chilling-treated plants and slightly increased in older ones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aebi, H.E.: Catalase.-In: Bergmayer, H.U. (ed.): Methods of Enzymatic Analysis. Pp. 273-286. Verlag Chemie, Weinheim 1983.

    Google Scholar 

  • Badiani, M., De-Biasi, M.G., Feliei, M.: Soluble peroxidase from winter wheat seedlings with phenoloxidase-like activity.-Plant Physiol. 92: 489-494, 1990.

    Google Scholar 

  • Chance, B., Maehly, A.C.: Assay of catalase and peroxidases.-Methods Enzymol. 2: 764-775, 1955.

    Google Scholar 

  • Drotar, A., Phelps, P. Fall, R.: Evidence for glutathione peroxidase activities in cultured plant cells.-Plant Sci. 42: 35-40, 1985.

    Article  CAS  Google Scholar 

  • Foyer, C.I., Halliwell, B.: The presence of glutathione and gluathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism.-Planta 133: 21-25, 1976.

    Article  Google Scholar 

  • Foyer, C.I., Descourvieres, P., Kunert, K.I.: Protection against oxygen radicals: an important defence mechanism studied in transgenic plants.-Plant Cell Environ. 17: 507-523, 1994a.

    Article  CAS  Google Scholar 

  • Foyer, C.I., Lelandais, M., Kunert, K.I.: Photooxidative stress in plants.-Physiol. Plant. 92: 696-717, 1994b.

    Article  CAS  Google Scholar 

  • Giannopolitis, C.N, Rise, S.K.: Superoxide dismutases. I. Occurrence in higher plants.-Plant Physiol. 59: 309-314, 1977.

    PubMed  CAS  Google Scholar 

  • Gossett, D.R., Millhollon, E.P., Lucas, M.: Antioxidant response, to NaCl stress in salt-tolerant and salt-sensitive cotton.-Crop Sci. 34: 706-714, 1994.

    Article  CAS  Google Scholar 

  • Greencia, R.P., Bramlag, W.J.: Reversibility of chilling injury to cotton seedlings.-Plant Physiol. 47: 389-392, 1971.

    Google Scholar 

  • Halliwell, B.: Oxidative damage, lipid peroxidation and antioxidant protection in chloroplasts.-Chem. Phys. Lipids 44: 327-340, 1987.

    Article  CAS  Google Scholar 

  • Hasaneen, M.N.A., El Saht, H.M.: Comparative effects of anion radicals on growth and mineral composition of Vicia faba and Zea mays plants.-J. agr. Sci. Mansoura Univ. 18: 1698-1707, 1993.

    Google Scholar 

  • Havaux, M., Davaud, A.: Photo-inhibition of photosynthesis in chilled potato leaves is not correlated with a loss of photosystem II activity.-Photosynth. Res. 40: 75-92, 1994.

    Article  CAS  Google Scholar 

  • Hodges, D.M., Andrews, C.J., Johnson, D.A., Haniton, R.I.: Antioxidant compound responses to chilling stress in differntially sensitive inbred maize lines.-Physiol. Plant. 98: 685-692, 1996.

    Article  CAS  Google Scholar 

  • Hodgson, R.A.J., Raison, J.K.: Superoxide production by thylakoids during chilling and its implication the susceptibility of plants to chilling induced photoinhibition.-Planta 183: 222-228, 1991.

    Article  CAS  Google Scholar 

  • Jennings, P., Saltveit, M.E.: Temperature and chemical shocks induce chilling tolerance of germinating Cucumis sativus (cv. Poinsett 76) seeds.-Physiol. Plant. 91: 703-707, 1994.

    Article  CAS  Google Scholar 

  • Knox, J.P., Dodge, A.D.: Singlet oxygen and plants.-Phytochemistry 24: 889-896, 1985.

    Article  CAS  Google Scholar 

  • Lichtenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes.-Methods Enzymol. 148: 350-382, 1987.

    Article  CAS  Google Scholar 

  • May, M.J., Leaver, C.J.: Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension cultures.-Plant Physiol. 103: 621-627, 1993.

    PubMed  CAS  Google Scholar 

  • Miller, K.W., Yang, C.S.: An isocratic high-performance liquid chromatography method for the simultaneous analysis of plasma retinol, tocopherol and various carotenoids.-Anal. Biochem. 145: 21-26, 1985.

    Article  PubMed  CAS  Google Scholar 

  • Mukherjee, S.P., Choudhuri, M.A.: Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings.-Physiol. Plant. 58: 166-170, 1983.

    Article  CAS  Google Scholar 

  • Nakano, Y., Asada, K.: Hydrogen peroxide is seavenged by ascorbate-specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.

    CAS  Google Scholar 

  • Öquist, G., Huner, N.P.A.: Cold-hardening induced resistance to photoinhibition of photosynthesis in winter rye is dependent upon an increased capacity for photosynthesis.-Planta 189: 150-156, 1993.

    Article  Google Scholar 

  • Rikin, A.A., Richmond, A.E.: Amelioration of chilling injury in cucumber seedlings by abscisic acid.-Physiol. Plant. 38: 95-97, 1976.

    Article  CAS  Google Scholar 

  • Robinson, J.M.: Does O2 photoreduction occur in chloroplasts in vitro?-Physiol. Plant. 72: 666-780, 1988.

    Article  CAS  Google Scholar 

  • Schoner, S., Krause, G. II.: Protective systems against active oxygen species in spinach: Response to cold acclimation in excess light.-Planta 180: 383-389, 1990.

    Article  Google Scholar 

  • Siegel, B.Z.: Plant peroxidase: an organismic perspective.-Plant Growth Regul. 12: 303-312, 1993.

    Article  CAS  Google Scholar 

  • Siegel, B.Z., Galston, A.W.: Biosynthesis of deuterated isoperoxidases in rye plants grown in D2O.-Proc. nat. Acad. Sci. USA 56: 1040-1042, 1966.

    Article  PubMed  CAS  Google Scholar 

  • Smith, I.K.: Stimulation of gluathione synthesis in photorespiratory plants by catalase inhibitors.-Plant Physiol. 79: 1044-1047, 1985.

    Article  PubMed  CAS  Google Scholar 

  • Snedecor, W., Cochran, G.: Statistical Methods.-Iowa State Univ. Press, Ames 1980.

    Google Scholar 

  • Walker, M.A., McKersie, B.D.: Role of the ascorbate-glutathione antioxidant system in chilling resistance of tomato.-J. Plant Physiol. 141: 234-239, 1993.

    CAS  Google Scholar 

  • Zhang, J., Cui, S., Li, J., Wei, J.: The effect of drought on superoxide dismutase in seedlings of wheat cultivars with different drought resistance.-Acta agr. Boreali sin. 5: 9-13, 1990.

    Google Scholar 

  • Zhang, J., Kirkham, M.B.: Drought-stress induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species.-Plant Cell Physiol. 35: 785-791, 1994.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, Faculty of Science, University of Mansoura, Mansoura, Egypt

    H. M. El-Saht

Authors
  1. H. M. El-Saht
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

El-Saht, H.M. Responses to chilling stress in French bean seedlings: antioxidant compounds. Biologia Plantarum 41, 395–402 (1998). https://doi.org/10.1023/A:1001850427160

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1001850427160

Search

Navigation