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Changes in Saccharide Metabolism Induced by Infection of Camellia Sinensis by Exobasidium Vexans

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Biologia Plantarum

Abstract

Changes in saccharide contents of tea leaves during infection with blister blight fungus Exobasidium vexans Masse was studied. Saccharose and glucose contents decreased in the blistered portions when compared to the normal regions until sporulation and remained constant during the entire period of sporulation. Fructose content increased abruptly during the initiation of sporulation and remained constant up to the end of sporulation in both blistered and non-blistered regions. Starch content continuously decreased in the blistered region. Peroxidase activity was highly enhanced during the final stages of leaf senescence. The activity of acid invertase was inversely proportional to the starch content and closely related to the changes in the saccharose and glucose contents. Protein and chlorophyll contents gradually decreased in the blistered regions.

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References

  • Agnibothrudu, V.: Some more important fungal disease of tea.-Indian Phytopathol. 48: 229-253, 1995.

    Google Scholar 

  • Bacon, J., Macdonald, T.R., Knight, A.H.: The development of invertase activity of root of Beta vulgariswashed under aseptic conditions.-Biochem. J. 94: 175-182, 1965.

    PubMed  CAS  Google Scholar 

  • Bergmeyer, H.U.: Methods of Enzymatic Analysis.-VCH, Weinheim 1988.

    Google Scholar 

  • Braber, J.M.: Catalase and peroxidase in primary bean leaves during development and senescence.-Z. Pflanzenphysiol. 97: 135-144, 1980.

    CAS  Google Scholar 

  • Daly, J.M., Inman, R.E., Livne, A.: Carbohydrate metabolism in higher plant tissue infected with obligate parasites.-Plant Physiol. 37: 531-538, 1962.

    Article  PubMed  CAS  Google Scholar 

  • Friedrich, S., Boyle, C.: Wirkung unterschiedlicher Luftfeuchten auf die Produktion und Keimung der Konidien von Erysiphe graminisf. sp. tritici in vitro.-Z. Pflanzenkr. Pflanzensch. 100: 180-188, 1993.

    Google Scholar 

  • Grabo, P.: Physiologische Untersuchungen zum Verständnis der Wirt-Parasit-Bezichung des Pathosystems Erbse (Pisum sativum) Achter Mehltau (Erysiphe pisi).-Ph.D. Thesis. Universität Braunschweig, Braunschweig 1993.

    Google Scholar 

  • Greenland, A.J., Lewis, D.H.: Changes in the activity and predominant molecular forms of acid invertase in oat leaves infected by crow rust.-Physiol. Plant Pathol. 22: 293-312, 1983.

    CAS  Google Scholar 

  • Herbet, D., Phipps, P.J., Strange, R.E.: Chemical analysis of microbial cells.-In: Norris, J.R., Ribbons, D.W. (ed.) Methods in Microbiology. Pp. 209-272. Academic Press, London 1971.

    Google Scholar 

  • Higgett, A.G., Nixon, D.A.: Enzymatic determination of blood glucose.-Biochem. J. 66: 120, 1957.

    Google Scholar 

  • Huysmans, C.P.: [Studies on the blister blight (Exobasidium vexans) in tea of Sumatra.]-Bergculture 21: 419-464, 1952. [In Dutch.]

    Google Scholar 

  • Inman, R.E.: Disease development, disease intensity and carbohydrate levels in rusted bean plants.-Phytopathology 52: 1207-1211, 1962.

    Google Scholar 

  • Kakac, B., Vejdelek, Z.: Handbuch der photometrischen Analyse organischer Verbindungen. Band 2.-VCH, Weinheim 1974.

    Google Scholar 

  • Kiessling, U., Hoffmann, G.M.: Physiologische Effekte bei Interaktionen zwischen Echtem Mehltau (Erysiphe graminisf. sp. hordei) und Zwergrost (Puccinia hordei) an Gerste.-Z. Pflanzenkr, Pftanzensch. 92: 407-416, 1985.

    Google Scholar 

  • Lichtenthaler, H.K.: Chlorophylls and carotenoids pigments of photosynthetic biomembranes.-Methods Enzymol. 148: 350-400, 1987.

    Article  CAS  Google Scholar 

  • Long, D.E., Fung, A.K., McGee, E., Cook, R.C., Lewis, D.H.: The activity of invertase and its revelance to the accumulation of storage polysaccharides in leaves by biotrophic fungi.-New Phytol. 74: 173-182, 1975.

    Article  CAS  Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951.

    PubMed  CAS  Google Scholar 

  • Manners, J.M.: The host-haustorium interface in powdery mildews.-Aust. J. Plant Physiol. 16: 45-52, 1989.

    Article  Google Scholar 

  • McKay, D.B., Maclean, D.J.: Effect of glucose and glucitol analogues on respiration by mycelia of Puccinia graminis.-Exp. Mycol. 16: 110-118, 1992.

    Article  CAS  Google Scholar 

  • Miidla, H., Padu, E., Kolok, U., Soosaar, A.: Biochemical changes in primary wheat leaves during growth and senescence.-Biol. Plant. 29: 445-452, 1987.

    CAS  Google Scholar 

  • Mitchell, D.T., Fung, A.K., Lewis, D.H.: Changes in the ethanol-soluble carbohydrate composition and acid invertase activity in infected first leaf tissues of susceptable crow rust of oat and wheat stem rust.-New Phytol. 80: 381-392, 1978.

    Article  CAS  Google Scholar 

  • Owera, S.A.P.P., Farrer, J.F., Whitebread, R.: Translocation from leaves infected with brown rust.-New Phytol. 94: 111-123, 1983.

    Article  CAS  Google Scholar 

  • Schipper, A.L., Mirocha, C.J.: The histochemistry of starch depletion and accumulation in bean leaves at rust infection sites.-Phytopathology 59: 1416-1422, 1969.

    Google Scholar 

  • Scholes, J.D., Farrar, D.H.: Development of symptoms of brown rust of barely in relation to the distribution of fungal mycelium, starch accumulation and localized changes in the concentration of chlorophyll.-New Phytol. 107: 103-117, 1987.

    Article  Google Scholar 

  • Scholes, J.D., Lee, P.J., Horton, P., Lewis, D.H.: Invertase: understanding changes in the photosynthetic and carbohydrate metabolism of barley leaves infected with powdery mildews.-New Phytol. 126: 213-222, 1994.

    Article  CAS  Google Scholar 

  • Storr, T., Hall, J.L.: The effect of infection by Erysiphe pisiDC on acid and alkali invertase activities and aspects of starch biochemistry in leaves of Pisum sativumL.-New Phytol. 121: 535-543, 1992.

    Article  CAS  Google Scholar 

  • Sziraki, I., Mustary, L.A., Faludi-Daniel, A., Kiraly, Z.: Alternatives in chloroplast ultrastructure and chlorophyll content in rust infected Pinto beans at different stages of disease development.-Phytopathology 74: 77-84, 1984.

    Google Scholar 

  • Van Handel, E.: Direct microdetermination of saccharose.-Anal. Biochem. 22: 280-283, 1968.

    Article  PubMed  Google Scholar 

  • Wagner, S., Boyle, C.: A method for simultaneous vital staining in obligate biotrophic interactions.-Z. Pflanzenkrankh. Pflanzensch. 102: 184-190, 1995.

    Google Scholar 

  • Whipps, J. M., Lewis, D.H.: Patterns of translocation, storage and interconversion of carbohydrates.-In: Ayres, P.G. (ed.): Effects of Disease on the Physiology of the Growing Plant. Pp. 47-84. Cambridge University Press, Cambridge 1981.

    Google Scholar 

  • Xiu, W.T., Rolfe, S.A., Scholes, J.D.: Changes in invertase activity and isoform pattern in leaves of Arabidopsis thalianainfected with Albugo candida.-Abstracts. 6thInternational Congress of Plant Pathology. P. 242. Montreal 1993.

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Pius, P., Krishnamurthy, K. & Nelson, R. Changes in Saccharide Metabolism Induced by Infection of Camellia Sinensis by Exobasidium Vexans . Biologia Plantarum 41, 127–132 (1998). https://doi.org/10.1023/A:1001785104994

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