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Influence of Inoculation of Leifsonia xyli subsp. xyli on Photosynthetic Parameters and Activities of Defense Enzymes in Sugarcane

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Abstract

Ratoon stunting disease of sugarcane, caused by Leifsonia xyli subsp. xyli (Lxx), is one of the most serious diseases that cause substantial losses in yield. The objective of this study was to investigate the influence of inoculation of Lxx on the photosynthetic parameters and activities of defense enzymes in sugarcane. Seedcanes were inoculated with Lxx and planted in pots. Photosynthetic parameters, specific leaf weight (SLW), superoxide anion radical (O2−) and activities of phosphoenolpyruvate carboxylase (PEPC), superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in leaves of sugarcane varieties Badila, GT11 and GT29 were measured at 150, 180, 210 and 240 days after Lxx inoculation. The agronomic traits were estimated after inoculation for 11 months at processing maturing stage of sugarcane. The Lxx-inoculated sugarcane showed significant reduction in SLW, PEPC activity, chlorophyll (SPAD index) and plant height from 150 to 240 days, in net photosynthetic rate (Pn) and transpiration rate (Tr) from 180 to 240 days, in stomatal conductance (Gs) at 180 and 240 days, and in intercellular CO2 concentration (Ci) on the 180th day after inoculation. The content of O2− and activities of SOD and POD also were increased, while the activity of CAT decreased for most time after inoculation.

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Acknowledgments

This research was financially supported by the National High Technology Research and Development Program of China (863 Program) (2013AA102604), National Natural Science Foundation of China (31360293), National Program for International Scientific Exchange (2013DFA31600) and Guangxi Funds for Bagui Scholars and Distinguished Experts.

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    Authors and Affiliations

    1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, College of Agriculture, Guangxi University, 100 East Daxue Road, Nanning, 530005, China

      Xiao-Qiu Zhang, Yong-Jian Liang, Kai Zhu, Chao-Xing Wu & Li-Tao Yang

    2. Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center, Chinese Academy of Agricultural Sciences-Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning, 530007, China

      Yang-Rui Li

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    Xiao-Qiu Zhang and Yong-Jian Liang have contributed equally to this work.

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    Zhang, XQ., Liang, YJ., Zhu, K. et al. Influence of Inoculation of Leifsonia xyli subsp. xyli on Photosynthetic Parameters and Activities of Defense Enzymes in Sugarcane. Sugar Tech 19, 394–401 (2017). https://doi.org/10.1007/s12355-016-0479-1

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