Abstract
The changes in nuclear ultrastructure during garlic development were investigated. In quiescent cells, the nucleus was oblate and small, full of heterochromatin, and without nucleolus. In the active phase cells, the nucleus moved toward the center of the cell, and was enlarged with many deep fissures, and the heterochromatin in the nucleus turned to be euchromatin. One or more nucleoli were seen in active phase cells. Cell divisions were by means of amitosis. Relatively large nuclei with small distortions were seen in the callus cells. These results suggest that the metabolism of the active cells was higher than that of callus and quiescent cells.
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References
Abo El-Nil, M.M.: Organogenesis and embryogenesis in callus of garlie (Allium sativumL.).-Plant Sci. Lett. 9: 259-264, 1977.
Bouteille, M., Bouvier, D., Seve, P.: Heterogeneity and territorial organization of the nuclear matrix and related structures.-Int. Rev. Cytol. 83: 135-182, 1983.
Crevecoeur, M., Hagege, D., Catesson, A., Greppin, H., Gaspar, T., Ultrastructural characteristic of cells from normal and habituated sugar beet calli.-Plant Physiol. Biochem. 30: 87-95, 1992.
Dominíque, F., Bonnelle, C., Tourt, T.: Ultrastructural features of soybean somatic cells at the beginning of an organogeneic process: toward a new concept.-Biol. Cell 73: 99-105, 1991.
Fransz, P.F., Schel, J.H.N.: An ultrastructural study of early callus development from immature embryos of maize strain A188 and A632.-Acta bot. neerl. 36: 247-260, 1987.
Johnson, J.M.: A study of nucleolar vacuoles in cultured tobacco cells using radioautography, actinomycine D, and electron microscopy.-J. Cell Sci. 43: 197-206, 1969.
Lafontaine, J.G.: Ultrastructural organization of plant cell nuclei.-In: Busch, H. (ed.): The Cell Nucleus. Vol. I, Pp. 149-185. Academic Press, New York-London 1974.
Ma, Y., Wang, H.-L., Zhang, C.-J., Kang, Y.-Q.: High rate virus-free plantlet regeneration via garlic scape-tip culture.-Plant Cell Rep. 14: 65-68, 1994.
Takahashi, Y., Kuroda, H., Tanaka, T., Machida, Y., Takebe, I., Nagata, T.: Isolation of an auxin-regulated gene cDNA expressed during the transition from G0to S phase in tobacco mesophyll protoplasts.-Proc. nat. Acad. Sci. USA 86: 9279-9283, 1989.
Takahashi, Y., Niwa, Y., Machida, Y., Nagata, T.: Location of the cis-acting auxin-responsive region in the promoter region of the par gene from tobacco mesophyll protoplasts.-Proc. nat. Acad. Sci. USA 87, 8013-8016, 1990.
Wang, H.-L., He, J.-Y., Ma, Y., Hong, R.-Y., Wang, T.-K.: An ultrastructural study and changes in peroxidase isozyme patterns during callus induction from garlic sprout leaf.-Acta Agr. Borealisin. 6: 74-80, 1991.
Wang, H.-L., Kang, Y.-Q., Zhang, C.-J., Ma, Y., Wang, T.-K.: Plant regeneration viatissue culture of garlic pedical.-Acta agr. Boreali-sin. 7: 66-70, 1992.
Wang, H.-L., Kang, Y.-Q., Zhang, C.J.: Embryogenesis via culture of garlic sprout leaf.-Acta Agr. Boreali-sin. 9: 92-94, 1994.
Weibroad, S.: Active chromatin.-Nature 297: 289-295, 1982.
Yeoman, M.M., Street, H.E.: General cytology of cultured cells.-In: Street, H.E. (ed.): Plant Tissue and Cell Culture. Pp. 137-176. Blackwell Scientific Publishers, Oxford 1977.
Zhang, C.-J., Ma, Y., Wang, H.-L., Yan, F.-Y.: Effects of growth regulators on chromosome ploidy variation in asparagus anther culture.-Acta agr. Boreali-sin. 7: 29-34, 1992.
Zhang, C.-J., Wang, H.-L., Ma, Y., Kang, Y.-Q.: Regeneration of haploid plants from isolated microspores of asparagus (Asparagus officinalis L.).-Plant Cell Rep. 13: 637-640, 1994.
Zybina, E.Y.: [The structure of nucleus and nuclcolus during ovogenesis of mice.]-Tsitologiya 10: 36-42, 1968. [In Russ.]
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Wang, HL., Kang, YQ., Zhang, CJ. et al. Changes in Nuclear Ultrastructure during Callus Development in Tissue Culture of Allium Sativum . Biologia Plantarum 41, 49–55 (1998). https://doi.org/10.1023/A:1001752214563
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DOI: https://doi.org/10.1023/A:1001752214563