Skip to main content
SpringerLink
Log in

Genomic fingerprints in the tribe Triticeae produced by PCR using a tRNA consensus primer

  • Published:
Biologia Plantarum

Abstract

Forty-one accessions belonging to ten genera of the tribe Triticeae representing both wild and cultivated species were analyzed by polymerase chain reaction (PCR). Of two consensus tRNA primers tested, one primer revealed characteristic amplification products for all of the species. A total of 35 tRNA markers were scored across all accessions. Five genus-specific and three species-specific markers were obtained. Genomic fingerprints were largely conserved within a genus. The phylogram obtained using parsimony has separated most of the accessions into their prevailing taxonomic species and genus groups. The phylogram showed close association among the three genera Secale, Triticum and Hordeum as expected. The Triticum-Secale relationship was closer than the Triticum-Hordeum or the Secale-Hordeum relationships. The tree also reflected the close associations among the forage grass species belonging to Leymus and Elymus. Thus tDNA-PCR helped to identify species and genera.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baum, B.R., Estess, J.R., Gupta, P.K.: Assessment of the genomic system of classification in the Triticeae.-Amer. J. Bot. 74: 1338-1395, 1987.

    Article  Google Scholar 

  • Botstein, D., White, R.L., Skolnick, M., Davis, R.W.: Construction of a genetic linkage map using restriction fragment length polymorphisms.-Amer. J. human Genet. 32: 314-331, 1980.

    CAS  Google Scholar 

  • Bowden, W.M.: The taxonomy and nomenclature of the wheats, barleys and ryes and their wild relatives.-Can. J. Bot. 37: 657-684, 1959.

    Article  Google Scholar 

  • Bowman, C.M., Bonnard, G., Dyer, T.A.: Chloroplast DNA variation between species of Triticum and Aegilops: location of the variation on the chloroplast genome and its relevance to the inheritance and classification of the cytoplasm.-Theor. appl. Genet. 65: 247-262, 1983.

    Article  CAS  Google Scholar 

  • Dewey, D.R.: The genomic system of classification of as a guide to intergeneric hybridization with the perennial Triticeae.-In: Gustafson, J.P. (ed.): Gene Manipulation in Plant Improvement. (Stadler Genet. Symp. 16). Pp. 209-280. Plenum Publ. Corp., New York 1984.

    Google Scholar 

  • Jensen, K.B., Bickford, I.W.: Cytology of intergeneric hybrids between Psathyrostachys and Elymus with Agropyron (Poaceae: Triticeae).-Genome 35: 676-680, 1992.

    Google Scholar 

  • Jinks-Robertson, S., Nomura, M.: Ribosomes and tRNA in E. coli and S. typhimurium.-In: Neidhardt, F.C. (ed.): Escherichia coli and Salmonella typhimurium Cellular and Molecular Biology. Pp. 1358-1385. ASM Press, Washington 1987.

    Google Scholar 

  • Kellog, E.A.: Tools for studying the chloroplast genome in the Triticeae (Gramineae): An EcoRI map, a diagnostic deletion, and support for Bromus as an outgroup.-Amer. J. Bot. 79: 186-197, 1992.

    Article  Google Scholar 

  • Mullis, K.B., Faloona, F.: Specific synthesis of DNA in vitro via a polymerase catalysed chain reaction.-Methods Enzymol. 155: 335-350, 1987.

    Article  PubMed  CAS  Google Scholar 

  • Murray, M.G., Thompson, W.F.: Rapid isolation of high molecular weight plant DNA.-Nucl. Acids Res. 8: 4321-4325, 1980.

    PubMed  CAS  Google Scholar 

  • Ogihara, Y., Tsunewaki, K.: Diversity and evolution of chloroplast DNA in Triticum and Aegilops as revealed by restriction fragment analysis.-Theor. appl. Genet. 76: 321-332, 1988.

    Article  CAS  Google Scholar 

  • Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W.: Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics.-Proc. nat. Acad. Sci. USA 81: 8014-8018, 1984.

    Article  PubMed  CAS  Google Scholar 

  • Saiki, R.K.: Principles and Application for DNA Amplification.-Stockton Press, New York 1989.

    Google Scholar 

  • Skolnick, M.H., Wallace, R.B.: Simultaneous analysis of multiple polymorphic loci using amplified sequence polymorphism (ASPs).-Genomics 2: 273-279, 1988.

    Article  PubMed  CAS  Google Scholar 

  • Tanksley, S.D., Young, N.D., Paterson, A.H., Bonierbale, M.W.: RFLP mapping in plant breeding: new tools for an old science.-Bio/Technology 7: 257-264, 1989.

    Article  CAS  Google Scholar 

  • Welsh, J., McClelland, M.: Genomic fingerprints produced by PCR with consensus tRNA gene primers.-Nucl. Acids Res. 19: 861-866, 1991.

    PubMed  CAS  Google Scholar 

  • Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. Tingey, S.V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.-Nucl. Acids Res. 18: 6531-6535, 1990.

    PubMed  CAS  Google Scholar 

  • Yang, X., Quiros, C.: Identification and classification of celery cultivars with RAPD markers.-Theor. appl. Genet. 86: 205-212, 1993.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Pathology, Cook College, Rutgers University, New Brunswick, NJ, 08903, USA

    P.V. Reddy & K.M. Soliman

Authors
  1. P.V. Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K.M. Soliman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P.V. Reddy.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Reddy, P., Soliman, K. Genomic fingerprints in the tribe Triticeae produced by PCR using a tRNA consensus primer. Biologia Plantarum 40, 55–65 (1997). https://doi.org/10.1023/A:1000992417115

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1000992417115

Search

Navigation