Abstract
The growth retardant paclobutrazol (PBZ) inhibited stem internode growth of in vitro cloned potato plants. The extent of growth inhibition caused by10-9 M PBZ in Murashige and Skoog medium was genotype-specific, varying between 10 - 60% of the stem growth of untreated controls in ten cultivars examined. An increase in percentage of de novo bud regenerating stem internode segments (SIS) as well as in the total number of buds per explant was observed in SIS taken from PBZ pretreated plants. PBZ applied directly into the regenerative media had no stimulatory effect on there generation process. We assume that the enhancing effect of PBZ on regeneration may be attributed to its interaction with cytokinin metabolism.
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Coleman, M., Waugh, R., Powell, W.: Genetical annalysis of in vitro cell and tissue culture response in potato.-Plant Cell Tissue Organ Cult. 23: 181–186, 1990.
Dalziel, J., Laurence, D.K.: Biochemical and biological effects of kaurene oxidase inhibitors, such as paclobutrazol.-Brit. Plant Growth Regul. Group 11: 43–57, 1984.
Hedden, P., Graebe, J.E.: Inhibition of gibberellin biosynthesis in cell-free homogenates of Cucurbita maxima endosperm and Malus pumila embryos.-J. Plant Growth Regul. 4: 111–122, 1985.
Fletcher, R.A., Hofstra, G.: Comparative fungitoxic and plant growth regulating properties of triazole derivatives.-Plant Cell Physiol. 27: 367–371, 1986.
Grossmann, K.: Plant growth retardants as tools in physiological research.-Physiol. Plant. 78: 640–648, 1990.
Grossmann, K., Hauser, C., Sauerbrey, E., Fritsch, H., Schmit, O., Jung, C.: Plant growth retardants as inhibitors of ethylene production.-J. Plant Physiol. 134: 538–543, 1989.
Grossmann, K., Kwiatkowski, H., Siebecker, H., Jung, J.: Regulation of plant morphology by growth retardants. Effects on phytohormone levels in soybean seedlings determined by immunoassay.-Plant. Physiol. 84: 1018–1021, 1987.
Izumi, K., Nakagasa, S., Kobayashi, M., Oshio, H., Sakurai, A., Takahashi, N.: Levels of IAA, cytokinins, ABA and ethylene in rice plants affected by a gibberellin biosynthesis inhibitor, uniconazole-P.-Plant Cell Physiol. 29: 97–104, 1988.
Jung, J., Luib, M., Sauer, H., Zeh, B., Rademacher, W.: Growth regulation in crop plants with new types of triazole compounds.-J. Agron. Crop Sci. 158: 324–332, 1987.
Opatrná, J., Opatrný, Z., Kostřica, P.: [Genotype-specific growth and differentiation response of potato stem explants.]-In: Griga, M. (ed.): Explantátové kultury ve šlechtění a produkci zemědělských plodin. Pp. 105–115. OSEVA, Šumperk 1990. [In Czech.]
Opatrný, Z., Müllerová, E.: Anatomical analysis of the organogenesis in potato (Solanum tuberosum L.).-Genet. Selekt. (Sofia) 5: 455–458, 1986.
Ovesná, J., Burdová, I., Opatrná, J., Opatrný, Z.: Modification of cultivar-specific regeneration capacity of potato explants by phytohormones and by Agrobacterium oncogenes.-Biol. Plant. 35: 199–208, 1993.
Novák P.: [The effect of paclobutrazol on growth processes in potato in vitro cultures.]-Thesis. Czech Agr. Univ., Prague 1994. [In Czech.]
Pinhero, R.G., Fletcher, R.A.: Paclobutrazol and ancymidol protect corn seedlings from high and low temperature stresses.-Plant Growth Regul. 15: 47–53, 1994.
Rademacher, W.: Biochemical effects of plant growth retardants.-In: Gausman, H.W. (ed.): Plant Biochemical Regulators. Pp. 169–200. Marcel Dekker, New York 1991.
Svojšová, J.: [The effect of retardants on the growth of potato explants.]-Thesis. Czech Agr. Univ., Prague 1991. [In Czech.]
Webb, K.J., Osifo, E.O., Henshaw, G.G.: Shoot regeneration from leaflet dises of six cultivars of potato (Solanum tuberosum subsp. tuberosum).-Plant Sci. Lett. 30: 1–8, 1983.
Werbrouck, S.P.O., Debergh, P.C.: Fungicides interacting with cytokinins.-Med. Fac. Landbouw. Univ. Gent 60/4a: 1547–1552, 1995.
Wheeler, V.A., Evans, N.E., Foulger, D., Webb, K.J., Karp, A., Franklin, J., Bright, W.J.: Shoot formation from explant cultures of fourteen potato cultivars and studies of the cytology and morphology of regenerated plants.-Ann. Bot. 55: 309–320, 1985.
Ziv, M.: Enhanced shoot and cormlet proliferation in liquid cultured Gladiolus buds by growth retardants.-Plant Cell Tissue Organ Cult. 17: 101–110, 1989.
Ziv, M.: The effect of growth retardants on shoot proliferation and morphogenesis in liquid cultured Gladiolus plants.-Acta Hort. 280: 207–214, 1990.
Ziv, M., Ariel, T.: Bud proliferation and plant regeneration in liquid-cultured Philodendron treated with Ancymidol and Paclobutrazol.-J. Plant Growth Regul. 10: 53–57, 1991.
Ziv, M., Yogev, T., Krebs, O.: Effects of paclobutrazol and chlormequat on growth pattern and shoot proliferation of normal and variant Aechmea faciata “Baker” plants regenerated in vitro.-Israel J. Bot. 35: 175–182, 1986.
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Opatrná, J., Novák, P. & Opatrný, Z. Paclobutrazol stimulates bud regeneration in Solanum tuberosum L. primary explant cultures. Biologia Plantarum 39, 151–158 (1997). https://doi.org/10.1023/A:1000385712937
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DOI: https://doi.org/10.1023/A:1000385712937