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Membrane Fluidity of the Pentachlorophenol-Mineralizing Sphingomonas sp. UG30

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Abstract

The effect of PCP and NaPCP on the cytoplasmic membranes of the PCP-mineralizing bacterium Sphingomonas sp. UG30 was assessed using fluorescence polarization and total cellular fatty acid analysis. Direct exposure of resting UG30 cells to PCP up to 250 ppm and NaPCP up to 1000 ppm did not cause any changes in the polarization ratios or the fatty acid profile of the UG30 cytoplasmic membranes. Growth of UG30 cells in the presence of 25 ppm NaPCP did not affect the total cellular fatty acid profile or membrane fluidity as observed by fluorescence polarization.

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Authors and Affiliations

  1. Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1

    T. J. Denich, L. A. Beaudette, M. B. Cassidy, H. Lee & J. T. Trevors

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  1. T. J. Denich
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  2. L. A. Beaudette
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  3. M. B. Cassidy
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  4. H. Lee
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  5. J. T. Trevors
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Correspondence to J. T. Trevors.

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Denich, T.J., Beaudette, L.A., Cassidy, M.B. et al. Membrane Fluidity of the Pentachlorophenol-Mineralizing Sphingomonas sp. UG30. Journal of Fluorescence 13, 385–391 (2003). https://doi.org/10.1023/A:1026160721851

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