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Route of Jejunal Mucosa Absorption of Trypsin Demonstrated by Immunofluorescence

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Abstract

Previous studies have demonstrated the absorption of porcine trypsin in isolated jejunal loops from male Wistar rats by open-loop perfusion. The possible routes of absorption were examined in the study reported here. Trypsin (0.5 mg/ml) was dissolved in tyrode solution and perfused at a rate of 0.5 ml/min, at 37 °C, for 40 min. Using immunoperoxidase and immunofluorescence techniques, strong reactivity towards anti-TLCK-trypsin antibody was demonstrated through out the enterocyte cytosol. The present data indicate that trypsin was absorbed by enterocytes, probably through a transcellular route.

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Authors and Affiliations

  1. Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Brasil

    Elizabeth L. Borges & Maria de Fátima Leite

  2. Departamento de Anatomia Patológica, Faculdade de Medicina, Brasil

    Alfredo J.A. Barbosa

  3. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil

    José B. Alves

Authors
  1. Elizabeth L. Borges
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  2. Maria de Fátima Leite
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  3. Alfredo J.A. Barbosa
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  4. José B. Alves
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Borges, E.L., de Fátima Leite, M., Barbosa, A.J. et al. Route of Jejunal Mucosa Absorption of Trypsin Demonstrated by Immunofluorescence. Histochem J 34, 525–528 (2003). https://doi.org/10.1023/A:1026020301650

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  • DOI: https://doi.org/10.1023/A:1026020301650

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