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Regulation of Glial Na+/K+-ATPase by Serotonin: Identification of Participating Receptors

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Abstract

The purpose of the present study was the characterization of the receptors participating in the regulatory mechanism of glial Na+/K+-ATPase by serotonin (5-HT) in rat brain. The activity of the Na+ pump was measured in four brain regions after incubation with various concentrations of serotoninergic agonists or antagonists. A concentration-dependent increase in enzyme activity was observed with the 5-HT1A agonist R (+)-2-dipropylamino-8-hydroxy-1,2,3, 4-tetrahydronaphthalene hydrobromide (8-OH-DPAT) in homogenates or in glial membrane enriched fractions from cerebral cortex and in hippocampus. Spiperone, a 5-HT1A antagonist, completely inhibited the response to 8-OH-DPAT but had no effect on Na+/K+-ATPase activity in cerebellum where LSD, a 5-HT6 agonist, elicited a dose-dependent response similar to that of 5-HT. In brainstem, a lack of reponse to 5-HT and other agonists was confirmed. Altogether, these results show that serotonin modulates glial Na+/K+-ATPase activity in the brain, apparently not through only one type of 5-HT receptor. It seems that the receptor system involved is different according to the brain region. In cerebral cortex, the response seems to be mediated by 5-HT1A as well as in hippocampus but not in cerebellum where 5-HT6 appears as the receptor system involved.

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Authors and Affiliations

  1. Facultad de Ciencias Naturales, U.A.Q., México

    Ma. Teresa Peña-Rangel

  2. Lab. de Bioquiímica, Centro Nacional de Rehabilitación-Ortopedia, Mexico

    Rosalio Mercado-C.

  3. Laboratory of Neurontogeny, Department of Physiology, Biophysics and Neurosciences, CINVESTAV, IPN, Fac. Medicine, U.A.Q. Av. Instituto Politécnico Nacional, 2508, Col. San Pedro Zacatenco, México, D.F, México

    Jorge Hernández-Rodríguez (Ph.D)

Authors
  1. Ma. Teresa Peña-Rangel
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  2. Rosalio Mercado-C.
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  3. Jorge Hernández-Rodríguez
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Peña-Rangel, M.T., Mercado-C., R. & Hernández-Rodríguez, J. Regulation of Glial Na+/K+-ATPase by Serotonin: Identification of Participating Receptors. Neurochem Res 24, 643–649 (1999). https://doi.org/10.1023/A:1021048308232

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