Abstract
Two plasmids, pND801 and pND802, encoding different restriction and modification systems were isolated from Lactococcus lactis ssp. lactis LL42-1 and Lactococcus lactis ssp. cremoris LC14-1, respectively. pND802 contained one Sphl restriction enzyme site and the whole plasmid was cloned into the Sphl site of the streptococcal/ E. coli shuttle vector pSA3 generating the plasmid pND803. pND803 was stably maintained in L.lactis MG1363 harbouring pND801. The combination of the two R/M systems within L.lactis MG1363 resulted in an additive resistance towards both isometric phage and prolate phage.
Similar content being viewed by others
References
Anderson, D.G. and McKay, L.L. (1983) Appl. Environ. Microbiol. 46: 549-552.
Birnboim, H.C. and Doly, J. (1979) Nucl. Acids. Res. 7: 1513-1523.
Daly, C., Fitzgerald, G.F. and Davis R. (1996) Biotechnology of lactic acid bacteria with special reference to bacteriophage resistance. In: Lactic Acid Bacteria: Genetics, Metabolism and ApplicationsAntonie van Leeuwenhoek. 70: 99-110.
Dao, M.L. and Ferretti, J.J. (1985) Appl. Environ. Microbiol. 49: 115-119.
Davies, F.L. and Gasson, M.J. (1984) Bacteriophages of dairy lactic acid bacteria. In: Advances in the Microbiology and Biochemistry of Cheese and Fermented Milkpp.127-151, Elsevier Applied Science Publishers, New York.
Dagert, M. and Erhlich, S.D. (1979) Gene 6: 23-28.
Efstathiou, J.D. and McKay, L.L. (1977) J. Bacteriol. 130: 257-265.
Gasson, M.J. (1983) J. Bacteriol. 154: 1-9.
Gautier, M. and Chopin, M.C. (1987) Appl. Environ. Microbiol. 53: 925-927.
Jarvis, A.W. and Klaenhammer, T.R. (1986) Appl. Environ. Microbiol. 51: 1272-1277.
Klaenhammer, T.R. (1989) J. Dairy Sci. 72: 3429-3443.
Powell, B.I., Achen, G.M., Hillier, J.A. and Davidson, E.B. (1988) Appl. Environ. Microbiol. 54: 655-660.
Sing, W.D. and Klaenhammer, T.R. (1991) J. Dairy Sci. 74: 1133-1144.
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
Terzaghi, B.E. and Sandine, W.E. (1975) Appl. Microbiol. 29: 807-813.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Su, P., Ng, A., Kennelly, V. et al. Additive Effects of Two Different Plasmid-Linked Restriction and Modification Systems in Lactococcus. Biotechnology Letters 20, 515–518 (1998). https://doi.org/10.1023/A:1005404716216
Issue Date:
DOI: https://doi.org/10.1023/A:1005404716216