Abstract
Interaction between humic substances from a Pampasic pond and carbohydrates was studied. Determinations were performed by gel chromatography using radiolabeled glucose. The results showed that upon lowering the pH the interaction decreases in relation to the steric arrangement that causes the diminution of the accessibility of the available sites. Results of displacement experiments carried out by addition of unlabeled glucose, galactose, saccharose and starch, confirmed that the interaction occurs mainly through labile non specific bonds and is, to a certain degree, independent of the carbohydrate molecular weight involved.
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Aiken, G. R., D. M. McKnight, R. L. Wershaw & P. MacCarthy, 1985. Humic substances in Soil, Sediment and Water. Geochemistry, Isolation, and Characterization. John Wiley & Sons, New York, 692 pp.
Albright, L. J. & J. W. Wintworth, 1973. Use of the heterotrophic activity technique as a measure of eutrophication. Envir. Pollut. 8: 59–72.
Carter, C. W. & I. H. Suffet, 1982. Binding of DDT to Dissolved Humic Materials. Envir. Sci. Technol. 16: 735–740.
Conzonno, V. H. & A. Fernández Cirelli, 1987. Soluble humic substances from the affluents of Chascomús Pond. Arch. Hydrobiol. 109: 305–314.
Conzonno, V. H. & A. Fernández Cirelli, 1988. Soluble humic substances from Chascomús Pond (Argentina). Factors influencing distribution and dynamics. Arch. Hydrobiol. 111: 467–473.
Conzonno, V. H. & A. Fernández Cirelli, 1995. Dissolved organic matter in Chascomús Pond (Argentina). Influence of calcium carbonate on humic acid concentration. Hydrobiologia 297: 55–59.
Conzonno, V., C. Di Risio, M. Tudino, R. Erra Ballsells & A. Fernández Cirelli, 1994. Cadmium and chromium interactions with aquatic humic substances from Argentine ponds. Fresenius Envir. Bull. 3: 1–5.
De Haan, H. & T. De Boer, 1978. A study of the possible interactions between fulvic acids, amino acids and carbohydrates from Tjeukemeer, based on gel filtration at pH 7.0. Wat. Res. 12: 1035–1040.
De Haan, H., G. Werlermark & T. De Boer, 1983. Effect of pH on molecular weight and size of fulvic acids in drainage water from peaty grassland in NW Netherlands. Plant and Soil 75: 63–73.
De Haan, H., R. I. Jones & K. Salonen, 1987. Does ionic strength affect the configuration of aquatic humic substances, as indicated by gel filtration? Freshwat. Biol. 17: 453–459.
Engebretson, R. R., T. Amos & R. V. Wandruszka, 1996. Quantitative Approach to Humic Acid Associations. Envir. Sci. Technol. 30: 990–997.
Hayes, M. H. B., P. MacCarthy, R. L. Malcolm & R. S. Swift, 1989. Humic Substances II. In Search of Structure. JohnWiley & Sons, New York, 764 pp.
Lytle, J. R. & E. M. Perdue, 1981. Free, Proteinaceous, and Humic-Bound Amino Acids in River Water Containing High Concentrations of Aquatic Humus. Envir. Sci. Technol., 15: 224–228.
Parsons, T. R. & J. D. H. Strickland, 1962. On the production of particulate organic carbon by heterotrophic processes in sea water. Deep-Sea Res. 8: 211–222.
Romero, M. C. & A. A. Mariazzi, 1985/86. Heterotrophic bacterial uptake on glucose and glycollate in an eutrophic pond (Chascomús, Argentina). Ecosur 12/13: 111–121.
Schlautman, M. A. & J. J. Morgan, 1993. Effects of Aqueous Chemistry on the Binding of Polycyclic Aromatic Hydrocarbons by Dissolved Humic Materials. Envir. Sci. Technol. 27: 961–969.
Suffet, H. I. & P. MacCarthy, 1989. Aquatic Humic Substances. In-fluence on Fate and Treatment of Pollutants. American Chemical Society, Washington, DC, 864 pp.
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Conzonno, V.H., Fernández Cirelli, A. Interaction between humic substances and carbohydrates in a Pampasic pond (Argentina). Hydrobiologia 379, 169–174 (1998). https://doi.org/10.1023/A:1003471618831
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DOI: https://doi.org/10.1023/A:1003471618831