Abstract
The decrease in inorganic phosphate concentration in bean (Phaseolus vulgaris L. cv. Złota Saxa) roots induced decrease in respiration rate. The decrease observed in ATP pool in phosphate deficient (-P) roots was greater than it would result from the decline in respiration and possible involvement of alternative pathway, suggesting an increased energy utilization for growth and ion uptake. Indeed, relative growth rate was higher in -P plants until 12 d of culture and later dropped to the rate similar to the control. Net nitrate uptake rate was higher in -P plants than in +P plants at the beginning of phosphate starvation, then during the prolonged culture it decreased rapidly in -P plants and after 19 d it was 8 times lower than that in the control. The decline in ATP production during prolonged phosphate starvation influenced NO3 - uptake more than root growth.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Atkin, O.K., Villar, R., Lambers, H.: Partitioning of electrons between the cytochrome and alternative pathways in intact roots.-Plant Physiol. 108: 1179-1183, 1995.
Bouma, T.J., Broekhuysen, A.G.M., Veen, B.W.: Utilization of Respiratory Energy in Higher Plants. Requirements for "Maintenance" and Transport Processes.-Thesis. Landbouw-Universitcit, Wageningen 1995.
Cataldo, D.A., Haroon, M., Schrader, L.E., Youngs, V.L.: Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid.-Commun. Soil Sci. Plant Anal. 6: 71-80, 1975.
Ciereszko, I., Gniazdowska, A., Mikulska, M., Rychter, A.M.: Assimilate translocation in bean plants (Phaseolus vulgaris L.) during phosphate deficiency.-J. Plant Physiol. 149: 343-348, 1996.
Crawford, N.M.: Nitrate: nutrient and signal for plant growth.-Plant Cell 7: 859-868, 1995.
Day, D.A., Krab, K., Lambers, H., Moore, A.L., Siedow, J.N., Wagner, A.M., Wiskich, J.T.: The cyanide-resistant oxidase: to inhibit or not to inhibit, that is the question.-Plant Physiol. 110: 1-2, 1996.
de Visser, R.: On the integration on plant growth and respiration.-In: Moore, A.L., Beechey, R.B. (ed.): Plant Mitochondria. Structural, Functional and Physiological Aspect. Pp. 331-340. Plenum Press, New York 1987.
Hoefnagel, M.H.N., Millar, A.H., Wiskich, J.T., Day, D.A.: Cytochrome and alternative respiratory pathways compete for electrons in the presence of pyruvate in soybean mitochondria.-Arch. Biochem. Biophys. 318: 394-400, 1995.
Imsande, J., Touraine, B.: N demand and the regulation of nitrate uptake.-Plant Physiol. 105: 3-7, 1994.
Klobus, G.: Nitrate uptake and activity of plasmalemma associated ATPase in Cucumis sativus L. roots.-Acta Physiol. Plant. 12: 225-231, 1990.
Kondracka, A., Rychter, A.M.: The role of Pi recycling processes during photosynthesis in phosphate-deficient bean plants.-J. exp. Bot. 48: 1461-1468, 1997.
McClure, P.R., Kochian, L.V., Spanswick, R.M., Shaff, J.E.: Evidence for cotransport of nitrate and protons in maize roots. I. Effect of nitrate on membrane potential.-Plant Physiol. 93: 281-289, 1990a.
McClure, P.R., Kochian, L.V., Spanswick, R.M., Shaff, J.E.: Evidence for cotransport of nitrate and protons in maize roots. II. Measurement of NO3 - and H+ fluxes with ion-selective microelectrodes.-Plant Physiol. 93: 290-294, 1990b.
Pradet, A.: Étude des adénosine-5-mono-, di-, et tri-phosphate dans les tissues végétaux. I. Dosage enzymatique.-Physiol. vég. 5: 209-221, 1967.
Rao, I.M., Fredeen, A.L., Terry, N.: Influence of phosphorus limitation on photosynthesis, carbon allocation and partitioning in sugar beet and soybean grown with a short photoperiod.-Plant Physiol. Biochem. 31: 223-231, 1993.
Rufty, T.W., Jr., MacKown, C.T., Israel, D.W.: Phosphorus stress effects on assimilation of nitrate.-Plant Physiol. 94: 328-333, 1990.
Rufty, T.W., Jr., Israel, D.W., Volk, R.J., Qiu, J., Sa, T.: Phosphate regulation of nitrate assimilation in soybean.-J. exp. Bot. 44: 879-891, 1993.
Rychter, A.M., Mikulska, M.: The relationship between phosphate status and cyanide-resistant respiration in bean roots.-Physiol. Plant. 79: 663-667, 1990.
Rychter, A.M., Chauveau, M., Bomsel, J-L., Lance, C.: The effect of phosphate deficiency on mitochondrial activity and adenylate levels in bean roots.-Physiol. Plant. 84: 80-86, 1992.
Rychter, A.M., Randall, D.D.: The effect of phosphate deficiency on carbohydrate metabolism in bean roots.-Physiol. Plant. 91: 383-388, 1994.
Santi, S., Locci, G., Pinton, R., Cesco, S., Varanini, Z.: Plasma membrane H+-ATPase in maize roots induced for NO3 − uptake.-Plant Physiol. 109: 1277-1283, 1995.
Van der Werf, A., Kooijman, A., Welschen, R., Lambers, H.: Respiratory energy costs for the maintenance of biomass, for growth and for ion uptake in roots of Carex diandra and Carex acutiformis.-Physiol. Plant. 72: 483-491, 1988.
Warner, R. L., Kleinhofs, A.: Genetics and molecular biology of nitrate metabolism in higher plants.-Physiol. Plant. 85: 245-252, 1992.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gniazdowska, A., Mikulska, M. & Rychter, A. Growth, nitrate uptake and respiration rate in bean roots under phosphate deficiency. Biologia Plantarum 41, 217–226 (1998). https://doi.org/10.1023/A:1001862513105
Issue Date:
DOI: https://doi.org/10.1023/A:1001862513105