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Leaf Gas Exchange of Bean (Phaseolus vulgaris L.) Seedlings Subjected to Manganese Stress

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Abstract

Manganese (Mn) is a microelement required for optimal growth of plants and involved in several metabolic processes, mainly in photosynthesis. In the present work, the phytotoxicity of Mn was studied in bean plants (Phaseolus vulgaris L., var. ‘Coco-blanc’) treated with various concentrations of MnCl2: 10 (control), 100 and 300 µM (excess). After 20 days, treatment with 300 µM Mn affects plant fresh biomass. Nevertheless, shoot/root ratios were similar in all treatments. Inhibition of growth in 300 µM Mn-treated plants is concomitant to a reduction in chlorophylls and carotenoids contents. Furthermore, there was no significant difference in light-saturated carbon assimilation (Amax) between leaves grown in 10 and 100 µM Mn. In contrast, the presence of 300 µM decreased significantly Amax, which suggest an inability of bean plants to maintain its photosynthesis in response to Mn excess. Simultaneously, stomatal conductance (gs) and transpiration (E) declined at 300 µM Mn as compared to other treatments. This inability to maintain photosynthesis did not result from a decrease in leaf thickness since specific leaf area (SLA) was similar in all treatments. Probably, photosynthesis inhibition in plants grown under Mn excess is the consequence of their lower stomatal conductance.

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ACKNOWLEDGMENTS

We gratefully acknowledge Pr. Zouhaier Nasr (Institut National de Recherche en Génie Rural, Eaux et forêts, Tunisia) for his technical help.

Funding

This research was supported by the Ministry of Higher Education and Scientific Research in Tunisia (project no. LR18ES38).

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Authors and Affiliations

  1. Laboratory of Plant Toxicology and Environmental Microbiology, Faculty of Sciences of Bizerte, University of Carthage, 7054, Zarzouna, Tunisia

    Y. Mahjoubi, M. Ben Massoud, O. Kharbech, N. Loussaief, A. Chaoui & W. Djebali

  2. Laboratoire de Gestion et de Valorisation de Produits Forestiers (LGVPF), Institut National de Recherche en Génie Rural, Eaux et forêts (INRGREF), Tunis, Tunisia

    T. Rzigui

Authors
  1. Y. Mahjoubi
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  2. T. Rzigui
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  3. M. Ben Massoud
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  4. O. Kharbech
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  5. N. Loussaief
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  6. A. Chaoui
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  7. W. Djebali
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Correspondence to T. Rzigui.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

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Abbreviations:Amax—maximum photosynthetic rate; An—net photosynthesis; E—transpiration; Fm—maximum fluorescence; Fo—minimum fluorescence; Fv/Fm—maximum photochemical efficiency of photosystem II; gs—stomatal conductance; LCP— light compensation point; PAR—photosynthetically active radiation; PPFD—photosynthetic photons flux density; SLA—specific leaf area; WUE—water-use-efficiency; Φ—apparent quantum yield.

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Mahjoubi, Y., Rzigui, T., Ben Massoud, M. et al. Leaf Gas Exchange of Bean (Phaseolus vulgaris L.) Seedlings Subjected to Manganese Stress. Russ J Plant Physiol 67, 168–174 (2020). https://doi.org/10.1134/S1021443720010100

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  • DOI: https://doi.org/10.1134/S1021443720010100

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