Abstract
A simple spectrophotometric method for determination of some antiviral drugs, such as acyclovir and amantadine hydrochloride in pure and dosage forms, have been developed. The proposed method is based on the reactions of the selected drugs and metals. Acyclovir was reacted with copper (II) and cobalt (II) using a borax/sodium pH 9 hydroxide buffer, and in a non-aqueous medium using 1% pyridine in methanol, respectively.The formed complexes were absorbed maximally at A, 290 nm and at X 287 nm. The method determined from 112–1620 μg/mL of acyclovir, with a mean percentage of recovery of 99.32 ± 0.63 and of 98.77 ± 0.70 for copper (II) and cobalt (III), respectively.The suggested method was applied to a Novirus capsule, and its validity was ascertained by the standard addition technique. In addition, amantadine hydrochloride was allowed to react with iron (III) at pH 3 adjusted with 0.1 M ammonium hydroxide. The formed complex absorbed maximally at λ 295 nm. This method determined from 94–940 μg/mL of amantadine hydrochloride with a mean percentage of recovery of 99.49 ± 0.57. The suggested method was applied to an Adamine capsule, and its validity was ascertained by the standard addition technique.
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REFERENCES
Schaeffer, H., Beauchamp, L., De Miranda, P., Elion, G.B., Bauer, D.J., and Collins, P., Nature (London), 1978, vol. 272, p. 583.
Park, N.-H., Pavan-Langston, D., and Hettinger, M.E., J. Dent. Res., 1980, vol. 59, p. 2080.
British Pharmacopoeia, London: Her Majesty's Stationary Office Pharmaceutical Press, 1993.
Nebinger, P. and Koel, M., J. Chromatogr., B, 1993, vol. 619, no. 2, p. 342.
Swart, K.J. and Hundt, H.K.L., J. Chromatogr., 1994, vol. 663, no. 1, p. 56.
Zhang, C. and Dong, S.N., Yaoxue xuebao, 1993, vol. 28, no. 8, p. 629.
Guan, R., Xiong, Z., and Yang, X., Yiyuo gongye, 1987, vol. 17, no. 7, p. 21.
Boulieu, R., Gallant, C., and Silberstein, J., J. Chromatogr., B, 1997, vol. 693, no. 1, p. 233.
Molokhia, A.M., Niazy, E.M., El-Hoofy, S.A., and El-Dardariy, M.E., J. Liq. Chromatogr., 1990, vol. 13, no. 5, p. 981.
Mascher, H., Kikuta, C., Metz, R., and Ergin, H., J. Chromatogr., B, 1991, vol. 121, p. 1.
Volpato, N.M., Santi, P., Laureri, C., and Colombo, P., J. Pharm. Biomed. Anal., 1997, vol. 16, no. 3, p. 515.
Mahrous, M.S., Abdel-Khalek, M.M., Daabees, H.G., and Beltagy, Y.A., Anal. Lett., 1992, vol. 25, no. 8, p. 1491.
Jouan-Rimbaud, D., Walczak, B., Massart, D.L., Last, I.R., and Prebble, K.A., Anal. Chim. Acta, 1995, vol. 304, no. 3, p. 285.
Ray, K. and Pradip, K., Indian J. Pharm. Sci., 1985, vol. 47, no. 2, p. 34.
Aoki, F.Y., Sitar, H.G., Boudreault, A., and Ogilvie, R.I., Clin. Pharmacol. Ther., 1985, vol. 37, p. 128.
Pacifici, G.M., Nardini, M., Ferrari, P., Latini, R., Fleschi, C., and Morselli, P.L., J. Clin. Pharmacol., 1976, vol. 3, p. 883.
United States Pharmacopoeia, Twenty Third Revision, Easton, Pa, United States Pharmacopeial Convention. Mack, 1995.
Biandrate, P., Tognoni, G., Belvedere, G., Frigerio, A., Rizzo, M., and Morselli, P.L., J. Chromatogr., 1972, vol. 74, p. 31.
Sioufi, A. and Pommier, F., J. Chromatogr., B, 1980, vol. 9, no. 1, p. 33.
Stead, A.H., Gill, R., Wright, T., Gibbs, J.P., and Moffat, A.C., Analyst (Cambridge, U.K.), 1982, vol. 107, p. 1106.
Bleidner, J.B., Harmon, W.E., Hewes, W.E., Lynes, T.E., and Herman, E.C., J. Pharmacol. Extl. Therap, 1965, vol. 150, p. 484.
Turcazan, J.W. and Medwiek, T., J. Pharm. Sci., 1974, vol. 63, p. 425.
Tusbouci, S., Gormara, T., and Iguchi, S., Bunseki kaqaku, 1970, vol. 19, p. 95.
Sinsheimer, J.E., Hong, D.D., Stewart, J.T., Fink, M.L., and Burckhalter, J.H., J. Pharm. Sci., 1971, vol. 60, p. 141.
Stevens, H.M., Color Tests., Clarke's Isolation and Identification of Drugs, 2-Nd Ed, London: Pharmaceutical Press, 1986, p. 135.
Moeller, T. and Bailar, C., Chemistry with Inorganic Qualitative Analysis, 2-nd Ed., New York: Academic, 1984, p. 119.
Yoe, J.H., Jones A.L., Ind. Eng. Chem., Prod. Res. Dev., Anal. Ed., 1944, vol. 16. p. 111.
Vosburgh, W.C. and Cooper, G.R., J. Am. Chem. Soc., 1941, vol. 63, p. 437.
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Mustafa, A.A., Abdel-Fattah, S.A., Toubar, S.S. et al. Spectrophotometric Determination of Acyclovir and Amantadine Hydrochloride through Metals Complexation1 . Journal of Analytical Chemistry 59, 33–38 (2004). https://doi.org/10.1023/B:JANC.0000011665.20059.4f
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DOI: https://doi.org/10.1023/B:JANC.0000011665.20059.4f