Abstract
A simple protocol for mass multiplication of Crataeva nurvala, a medicinal tree, from seedling-derived explants is described. Six different types of explants (cotyledonary nodes, epicotyl nodes, hypocotyl segments, first pair of leaves, cotyledons, and root segments) developed shoots on Murashige and Skoog's (MS) basal medium or the same supplemented with different concentrations of 6-benzylaminopurine (BAP). Among the explants tested for caulogenic potential, only the epicotyl and cotyledonary nodal explants developed shoots on MS basal medium, while on BAP (0 – 2.0 mg dm−3) adjuvated media all the explants exhibited caulogenesis. The optimum concentration of BAP varied for these explants. The shoots could be rooted on half strength MS with 0.02 mg dm−3 α-naphthalene acetic acid to get plants, which have been transferred to soil. The explants from in vitro regenerated shoots also possessed a similar caulogenic potential.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahee, J., Duhoux, E.: Root culturing of Faidherbia = Acacia albida as a source of explants for shoot regeneration.-Plant Cell Tissue Organ Cult. 36: 219–225, 1994.
Babber, S., Mittal, K., Ahlawat, R., Varghese, T.M.: Micropropagation of Cardiospermum halicacabum.-Biol. Plant. 44: 603–606, 2001.
Bhat, S.R., Chitralekha, P., Chandel, K.P.S.: Regeneration of plants from long-term root culture of lime, Citrus aurantifolia (Christm.) Swing.-Plant Cell Tissue Organ Cult. 29: 19–25, 1992.
Burger, D.W., Hackett, W.P.: Gradients of adventitious bud formation on excised epicotyl and root section of Citrus.-Plant Sci. 43: 229–232, 1986.
Inamdar, J.A., Nataraj, M., Mohan, J.S.S., Subramanian, R.B.: Somatic embryogenesis from callus cultures of Crataeva nurvala Buch. Ham.-Phytomorphology 40: 319–322, 1990.
Jaiwal, S.P.K., Gulati, A., Dahiya, S.: Direct organogenesis in hypocotyl cultures of Tamarindus indica.-Biol. Plant. 41: 331–337, 1998.
Manzanera, J.A., Pardos, J.A.: Micropropagation of juvenile and adult Quercus suber L.-Plant Cell Tissue Organ Cult. 21: 1–8, 1990.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture.-Physiol. Plant. 15: 473–497, 1962.
Purohit, S.D., Tak, K., Kukda, G.: In vitro propagation of Boswellia serrata Roxb.-Biol. Plant. 37: 335–340, 1995.
Sharma, V., Padhya, M.A.: In vitro rapid multiplication and propagation of Crataeva nurvala.-Indian J. exp. Biol. 34: 243–246, 1996.
Subbaiah, M.M., Minocha, S.C.: Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis.-Plant Cell Rep. 9: 370–373, 1990.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Walia, N., Sinha, S. & Babbar, S. Micropropagation of Crataeva nurvala . Biologia Plantarum 46, 181–185 (2003). https://doi.org/10.1023/A:1022882006682
Issue Date:
DOI: https://doi.org/10.1023/A:1022882006682