Abstract
Polysaccharides (0.5, 1 and 3 mg ml−1) from cultured broth and mycelia of Phellinus linteus inhibited cytochrome P450 (CYP) 1A1, CYP 1A2, CYP 2B1, and CYP 2E1 activities in rat liver microsomes. The polysaccharides from the broth of Phellinus linteus grown with 5% (v/v) mulberry extract had highest inhibitory potency for CYP 1A1, 1A2 and 2B1 activities. The most potent inhibitor of CYP 2E1 activity were the polysaccharides from the broth of Phellinus linteusgrown with 10% (v/v) mulberry extract.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Burke MD, Thompson S, Elcombe CR, Halpert J, Haaparanta T, Mayer RT (1985) Ethoxy-, pentoxy-and benzyloxyphenoxazones and homologues: a series of substrates to distinguish between different induced cytochromes P-450. Biochem. Pharmacol. 34: 3337–3345.
Chihara G, Himuri J, Maeda YY, Arai Y, Fukuoka F (1970) Fractionation and purification of the polysaccharides with marked antumor activity, especially, lentinan, from Lentinus edodes (Berk) SING. Cancer Res. 30: 2776–2781.
Dicker E, McHugh Y, Cederbaum AI (1990) Increased oxidation of p-nitrophenol and aniline by intact hepatocytes isolated from pyrazole-treated rats. Biochim. Biophys. Acta 1035: 249–256.
Guengerich FP (1988) Roles of cytochrome P450 enzymes in chemical carcinogenesis and cancer chemotherapy. Cancer Res. 48: 2946–2954.
Guo Z, Smith TJ, Wang E, Eklind KI, Chung FL, Yang CS (1993) Structure activity relationships of arylalkyl isothiocyanates for the inhibition of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone metabolism and the modulation of xenobioticmetabolising enzymes in rats and mice. Carcinogenesis 14: 1167–1173.
Han MW, Ko KS, Chung KS (1995) Liquid cultivation of Phellinus linteus mycelium and preparation of antitumor and immunostimulating substance. Korea Patent No. 95–7860.
Hong WK, Sporn MB (1997) Recent advances in chemoprevention of cancer. Science 278: 1073–1077.
Shimada T, Yun CH, Yamazaki H, Gautier JC Beaune PH, Guengerich FP (1992) Characterization of human lung microsomal cytochrome P450 1A1 and its role in the oxidation of chemical carcinogens. Mol. Pharmacol. 41: 856–864.
Shon YH, Nam KS (2002) Cancer chemoprevention: inhibitory effect of soybeans fermented with basidiomycetes on 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate-induced mouse skin carcinogenesis. Biotechnol. Lett. 24: 1005–1010.
Shon YH, Jang EJ, Nam KS (2001) Liquid culture conditions for the production improvement of mycelia of Phellinus linteus. Korea Patent No. 10–2001–14512.
Shou M, Korzekwa KR, Krausz KW, Buters JTM, Grogan J, Goldfarb I, Hardwick JP, Gonzalez FJ, Gelboin HV (1996) Specificity of cDNA-expressed human and rodent cytochrome P450s in the oxidative metabolism of the potent carcinogen 7,12-dimethylbenz[a]anthracene. Mol. Carcinog. 17: 241–249.
Tabata K, Itoh W, Kojima T, Kawabata S, Misaki K (1981) Ultrasonic degradation of schizophyllan, an antitumor polysaccharide produced by Schizophyllum commune FRIES. Carbohyd. Res. 89: 121–135.
Tsukagoshi S, Ohashi F (1974) Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma 180 and rat ascites hepatoma AH-13 by oral use. Jpn. J. Cancer Res. (Gann) 65: 557–558.
Yang CS, Smith TJ, Hong JY (1994) Cytochrome P450 enzymes as targets for chemoprevention against chemical carcinogenesis and toxicity: opportunities and limitations. Cancer Res. 54(Suppl.): 1982s–1986s.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shon, YH., Nam, KS. Inhibition of cytochrome P450 isozymes in rat liver microsomes by polysaccharides derived from Phellinus linteus . Biotechnology Letters 25, 167–172 (2003). https://doi.org/10.1023/A:1021935922722
Issue Date:
DOI: https://doi.org/10.1023/A:1021935922722