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Heat Shock Induced Lipid Changes and Solute Leakage in Germinating Seeds of Pigeonpea

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Biologia Plantarum

Abstract

Heat shock (HS) reduced total lipid and phospholipid contents and their synthesis in germinating seeds of pigeonpea [Cajanus cajan (L.) Millspaugh]. Lipid peroxidation was also enhanced with increasing temperature and HS duration. HS influenced lipid metabolism to a higher extent at 45°C than at 40°C. This altered lipid metabolism and lipid peroxidation was associated with the loss of various solutes from the germinating seeds, and modification of growth and development. Pretreatment of germinating seeds at 40°C for 1 h or at 45°C for 10 min followed by incubation at 28°C for 3 h prior to 45°C for 2 h ameliorated solute leakage due to reduced lipid peroxidation and improvement in lipid content and membrane function.

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References

  • Aid, F., Demandre, C., Kesri, B.G., Mazliak, P.: Effects of heat shock on foliar lipid composition of rape: main molecular species modifications.-Plant Physiol. Biochem. 33: 235–240, 1995.

    Google Scholar 

  • Aid, F., Kesri, B.G., Demandre, C., Mazliak, P: Modification of the biosynthesis of rape lipid molecular species by heat shock.-Phytochemistry. 47: 1195–1200, 1998.

    Google Scholar 

  • Bartlett, G.R.: Phosphorus assay in column chromatography.-J. biol. Chem. 234: 466–468, 1959.

    Google Scholar 

  • Benzioni, A., Itai, C.: Short and long term effects of high temperatures (47–49 °C) on tobacco leaves. III. Efflux and P32 incorporation into phospholipids.-Physiol. Plant. 28: 493–497, 1973.

    Google Scholar 

  • Bhattacharjee, S., Mukherjee, A.K.: The deleterious effects of high temperature during early germination on membrane integrity and subsequent germination of Amaranthus lividus.-Seed Sci. Technol. 26: 1–8, 1998.

    Google Scholar 

  • Björkman, O.: The response of photosynthesis to temperature.-In: Grace, J., Ford, E.D., Jarvis, P.G. (ed.): Plants and Their Atmospheric Environment. Pp. 273–301. Blackwell Scientific Publishers, London 1980.

    Google Scholar 

  • Bligh, E.G., Dyer, W.J.: A rapid method of total lipid extraction and purification.-Can. J. Biochem. Physiol. 59: 888–893, 1959.

    Google Scholar 

  • Chen, H.H., Shen, Z.Y., Li, P.H.: Adaptability of crop plants to high temperature stress.-Crop Sci. 22: 719–725, 1982.

    Google Scholar 

  • Chen, Y.M., Kamisaka, S., Masuda, Y.: Enhancing effects of heat shock and gibberellic acid on the thermotolerance in etiolated Vigna radiata. I. Physiological aspects on thermotolerance.-Physiol. Plant. 66: 595–601, 1986.

    Google Scholar 

  • Dhindsa, R.S.: Inhibition of protein synthesis by products of lipid peroxidation.-Phytochemistry 21: 309–313, 1982.

    Google Scholar 

  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substances.-Anal. Chem. 28: 350–356, 1956.

    Google Scholar 

  • Guo, T., Wang, C., Zhu, Y., Wang, H., Li, J., Zhou. J., Guo. T.C., Wang, C.Y., Zhu, Y.J., Wang, H.C., Li, J.X., Zhou, J.Z.: Effects of high temperature on the senescence of roots and tops of wheat plants in the later stage.-Acta agron. sin. 24: 957–962, 1998.

    Google Scholar 

  • Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189–198, 1968.

    Google Scholar 

  • Howarth, C.J.: Heat shock proteins in sorghum and pearl millet, ethanol, sodium arsenite, sodium malonate and the development of thermotolerance.-J. exp. Bot. 41: 877–883, 1990.

    Google Scholar 

  • Howarth, C.J., Ougham, H.J.: Gene expression under temperature stress.-New Phytol. 125: 1–26, 1993.

    Google Scholar 

  • Kleinschmidt, M.G., McMohan., V.A.: Effect of growth temperature on the lipid composition of Cyanidium caldorium. I. Class separation of lipids.-Plant Physiol. 46: 286–289, 1970.

    Google Scholar 

  • Kurganova, L.N., Veselov, A.P., Goncharova, T.A., Sinitsyna, Y.V.: Lipid peroxidation and anti oxidant systems of protection against heat shock in pea [Pisum sativum (L.)] chloroplasts.-Russian J. Plant Physiol. 44: 630–635, 1997.

    Google Scholar 

  • Leopold, A.C.: Temperature effects on soybean imbibition and leakage.-Plant Physiol. 65: 1096–1098, 1980.

    Google Scholar 

  • Levitt, J.: Responses of Plants to Environmental Stress, Vol. 1: Chilling, Freezing and High Temperature Stress.-Academic Press, New York 1980.

    Google Scholar 

  • Lin, C., Chen, Y., Key, J.L.: Solute leakage in soybean seedlings under various heat shock regimes.-Plant Cell Physiol. 26: 1493–1498, 1985.

    Google Scholar 

  • Lin, C., Roberts, J.K., Key, J.L.: Acquisition of thermotolerance in soybean seedlings.-Plant Physiol. 74: 152–160, 1984.

    Google Scholar 

  • Manwaring, J.D., Csallany, A.S.: Malondialdehyde containing proteins and their relationship to vitamin E.-Lipids 23: 651–654, 1988.

    Google Scholar 

  • Marmiroli, N., Lorenzoni, C., Cattivelli, L., Stanca, A.M., Terzi, V.: Induction of heat shock proteins and acquisition of thermotolerance in barley (Hordeum vulgare L.). Variations associated with growth habit and plant development.-J. Plant Physiol. 135: 267–273, 1990.

    Google Scholar 

  • Martineau, J.R., Specht, J.E., Williams, J.H., Sullivan, C.Y.: Temperature tolerance in soybeans. I. Evaluation of a technique for assessing cellular membrane thermostability.-Crop Sci. 19: 75–78, 1979.

    Google Scholar 

  • Moore, S., Stein, W.H.: Photometric ninhydrin method for use in the chromatography of amino acids.-J. biol. Chem. 176: 367–388, 1954.

    Google Scholar 

  • Murphy, J.N., Noland, T.L.: Temperature effects on seed imbibition and leakage mediated by viscosity and membranes.-Plant Physiol. 69: 428–431, 1982.

    Google Scholar 

  • Ordin, L., Itai, C., Benzioni, A., Musolan, C., Kindinger, J.I.: Effect of heat shock on plant growth and on lipid and β-glucan synthesis.-Plant Physiol. 53: 118–121, 1974.

    Google Scholar 

  • Ougham, H., Stoddart, J.L.: Synthesis of heat-shock protein and acquisition of thermotolerance in high temperature tolerant and high temperature susceptible lines of Sorghum.-Plant Sci. 44: 163–167, 1986.

    Google Scholar 

  • Schlesinger, M.J., Santoro, M.G., Garaci, E.: Stress Proteins. Induction and Function.-Springer-Verlag, Berlin 1990.

    Google Scholar 

  • Skipski, V.P., Barclay, M.: Thin layer chromatography of lipids.-In: Lowenstein, J.M. (ed.): Methods in Enzymology. Pp. 542-548. Academic Press, London 1969.

    Google Scholar 

  • Sridevi, V., Satyanarayana, N.V., Madhava Rao, K.V.: Induction of heat shock proteins and acquisiton of thermotolerance in germinating pigeonpea seeds.-Biol. Plant. 42: 589–597, 1999.

    Google Scholar 

  • Sullivan, C.Y., Ross, W.M.: Selecting for drought and heat resistance in grain sorghum.-In: Mussel, H., Staples, R.C. (ed.): Stress Physiology in Crop Plants. Pp. 263–281. John Wiley and Sons, New York 1979.

    Google Scholar 

  • Towill, L.E., Mazur, P.: Studies on the reduction of 2,3,5-triphenyl tetrazolium chloride as a viability assay for plant tissue cultures.-Can. J. Bot. 53: 1097–1102, 1974.

    Google Scholar 

  • Upadhyaya, A., Davis, T.D., Sankla, N.: Heat shock tolerance and anti-oxidant activity in moth bean seedlings treated with tetcyclacis.-Plant Growth Regul. 10: 215–222, 1991.

    Google Scholar 

  • Wu, M.T., Wallner, S.J.: Heat stress responses in cultured plant cells.-Plant Physiol. 72: 817–820, 1983.

    Google Scholar 

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Madhava Rao, K., Sridevi, V. & Satyanarayana, N. Heat Shock Induced Lipid Changes and Solute Leakage in Germinating Seeds of Pigeonpea. Biologia Plantarum 45, 71–76 (2002). https://doi.org/10.1023/A:1015184004665

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