Abstract
The structure of synaptonemal complexes (SCs) of chromosomes of mouse primary spermatocytes were studied using electron microscopy on days 1, 10, and 36 after the completion of per os administration of drugs belonging to three groups of antibiotics: tetracyclins, macrolides, and fluoroquinolones. The antibiotics were administered to mice during ten days. At the substages of early and middle pachytene, heteromorphic SC bivalents and fragments of chromosome-core elements were detected in spermatocytes at all times studied after the administration of the antibiotics of three groups. As cells passed through the period from early to middle pachytene, the number of cells containing heteromorphic SC bivalents and the fragments of axial cores gradually decreased, which could be an indication of selection of cells with chromosomal aberrations. A high level of associations between the X chromosome and autosome bivalents (including heteromorphic ones) also favors this suggestion. A gradual decrease in the number of chromosomal aberrations was detected, as time elapsed from the completion of antibiotics administration. The study of sperm obtained from epididymises of males did not reveal significant differences in both morphology and motility of sperm between males of the control and experimental groups.
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REFERENCES
Nagata, R., Nagata, T., Onishi, M., et al., Chronic Toxicity and Recovery Tests of Rokitamycin in Beagle Dogs, Int. J. Androl., 1987, vol. 40, no. 3, pp. 561-587.
Watanabe, T., Fujikawa, K., Harada, S., et al., Reproductive Toxicity of the New Antibacterial Agent Levofloxacin in Rats and Rabbits, Arzneimittelforschung, 1992, vol. 43, no. 3A, pp. 374-377.
Guay, D.R., Patterson, D.R., Seipman, N., and Craft, J.C., Overview of the Tolerability of Clarithromycin in Preclinical and Clinical Trials, Drug Saf., 1993, vol. 8, no. 5, pp. 350-364.
Dfeker, H.W., Strafton, W.G., McGoman, M.P., et al., A Controlled Triad of the Use of Erythromycin for Men with Astenospermia, Int. J. Androl., 1984, vol. 7, no. 5, pp. 383-388.
Schramm, P., Schopf, R.E., and Wildfeuer, A., Josamycin Concentration in Human Ejaculate and Its Influence on Sperm Motility: A Contribution to Antibiotic Therapy in Andrological Patients, Andrologia, 1988, vol. 20, no. 6, pp. 521-525.
Maura, A. and Pino, A., Induction of Sperm Abnormalities in Mice by Norfloxacin, Mutat. Res., 1991, vol. 264, no. 4, pp. 197-200.
Ignat'eva, E.L. and Kurilo, L.F., Histological Examination of the Reproductive Function of the Ovaries in Adult Mice Subjected to Antenatal Administration of Oxytetracycline, Byul. Eksp. Biol. Med., 1984, vol. 97, no. 5, pp. 608-610.
Ignat'eva, E.L. and Kurilo, L.F., Gonadotoxic Effect of Oxytetracycline on Early Antenatal Oogenesis in Mice in Experiments in Vivo and in Vitro, Farmakol. Toksikol., 1984, no. 5, pp. 75-77.
Kushnuruk, Yu.I., Gonadotoxicity of Several Antibiotics and Possible Means for Its Prevention, Cand. Sci. (Biol.) Dissertation, Kiev, 1974.
Moses, M.J., Microspreading and Synaptonemal Complex in Cytogenetic Study, Chromosomes Today, 1977, vol. 6, pp. 71-82.
Backer, L.C., Gibson, J.B., Moses, M.J., and Allen, J.W., Synaptonemal Complex Damage in Relation to Meiotic Chromosome Aberration after Exposure of Male Mice to Cyclophosphamide, Mutat. Res., 1988, vol. 203, pp. 317-330.
Tepperberg, J.H., Moses, M.J., and Nath, J., Colchicine Effects on Meiosis in Male Mouse: I. Meiotic Prophase: Synaptic Arrest, Univalents, Loss of Damaged Spermatocytes, and a Possible Checkpoint at Pachytene, Chromosoma, 1997, pp. 183-192.
Tepperberg, J.H., Moses, M.J., and Nath, J., Colchicine Effects on Meiosis in Male Mouse: II. Inhibition of Synapsis and Induction of Nondisjunction, Mutat. Res., 1999, vol. 429, pp. 93-105.
Cawood, A.H. and Breckon, G., Synaptonemal Complex as Indicator of Structural Change in Chromosomes after Irradiation of Spermatogonia, Mutat. Res., 1983, vol. 122, pp. 149-154.
Kalikinskaya, E.I., Bogdanov, Yu.F., Kolomiets, O.L., and Shevchenko, V.A., Synaptonemal Complex-Based Analysis of Chromosome Rearrangements in the Progeny of Mice Exposed to γRays, Genetika (Moscow), 1986, vol. 22, no. 7, pp. 1119-1126.
Kolomiets, O.L., Mazurova, T.F., Pomerantseva, M.D., et al., Electron Microscopic Analysis of the Synaptonemal Complexes in Male Laboratory Mice Exposed in the Chernobyl Region during Embryogenesis, Genetika (Moscow), 1992, vol. 28, no. 9, pp. 120-127.
Allen, J.W., Gibson, J.B., Poorman, P.P., et al., Synaptonemal Complex Damage Induced by Clustogenic and Anti-Mitotic Chemicals: Implications for Nondisjunctions and Aneuploidy, Mutat. Res., 1988, vol. 201, pp. 313-324.
Navarro, J., Vidal, F., Quitart, M., et al., A Method for the Sequential Study of CK by Light and Electron Microscopy, Hum. Genet., 1981, vol. 59, pp. 419-423.
Chandley, A.C., A Model for Effective Pairing and Recombination at Meiosis Based on Early Replication Sites (R-Bands) along Chromosomes, Hum. Genet., 1986, vol. 72, pp. 50-57.
Kurilo, L.F., Shileiko, L.V., Mkhitarova, E.V., and Ostroumova, T.V., Structure of Hereditary Disorders of the Reproductive System in Patients with Disturbed Reproduction, Tezisy dokladov Rossiiskoi nauchno-prakticheskoi konferentsii “Mediko-geneticheskoe konsul'tirovanie v profilaktike nasledstvennykh boleznei” (Proc. Ross. Conf. “Medical Genetic Counseling in Prevention of Hereditary Disorders”), Moscow, 1997, pp. 154-155.
Monesi, V., Metabolismo dell'acide ribonucleice durante lo sviluppo delle cellule germinal maschili e nelle cellule del sertoli nel topo adulte, Arch. Ital. Anatomy, E.: Embriol., 1964, vol. 69, pp. 89-118.
Forejt, J., X-Inactivation and Its Role in Male Sterility, Chromosomes Today, 1984, vol. 8, pp. 17-30.
Kralewski, M., Novello, A., and Benavente, R., A Novel Mr 77 000 Protein of the XY Body of Mammalian Spermatocytes: Its Localization in Normal Animals and in the Searle's Translocation Carriers, Chromosoma, 1997, vol. 106, pp. 160-167.
Rosenmann, A., Wahrman, J., Richler, C., et al., Meiotic Association between the XY Chromosomes and Unpaired Autosomal Elements as a Cause of Human Male Sterility, Cytogenet. Cell Genet., 1986, vol. 39, no. 1, pp. 19-29.
Marchetti, F., Bishop, J.V., Shelby, M.D., et al., DAPI Analysis of Mouse Zygotes Confirms That Etoposide Treatment of Male Germ Cells Results in Dominant Lethality nut Not Heritable Translocations, Environ. Mol. Mut., 1998, vol. 31, no. 29, p. 60.
Kurilo, L.F., Dubinskaya, V.P., Ostroumova, T.V., et al., Analysis of Spermatogenesis by Immature Germ Cells in Ejaculate, Probl. Reprod., 1995, vol. 1, no. 3, pp. 33-38.
Khil'kevich, L.V., Kurilo, L.F., and Sheveleva, G.A., Effect of Antenatal Exposure to Nicotine on Male Germ Cells in Wistar Rats, Tsitol. Genet., 1993, vol. 27, no. 2, pp. 42-46.
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Kolomiets, O.L., Abuduev, N.K., Mazurova, T.F. et al. Damaging Effect of Antibiotics on the Structure of Synaptonemal Complexes of Meiotic Mouse Chromosomes. Russian Journal of Genetics 37, 141–149 (2001). https://doi.org/10.1023/A:1009085704439
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DOI: https://doi.org/10.1023/A:1009085704439