Skip to main content
SpringerLink
Log in

In vitro propagation of Syzygium travancoricum Gamble – an endangered tree species

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Rapid multiplication in Syzygium travancoricum, an endangered tree species was achieved from nodal explants on Murashige and Skoog's (MS) medium supplemented with Woody Plant Medium (WPM) micronutrients and plant growth regulators. Multiple shoot induction from young 1–2-year-old seedlings was observed on basal medium supplemented with 17.7 μM benzyladenine and 1.3 μM α-naphthalene acetic acid. A high number of multiple shoots (25 shoots/nodal explant) was observed by the third subculture on multiplication medium. The dark brown nodular callus obtained from in vitro grown shoots through axillary buds regenerated at a high frequency when transferred to 1/2 strength basal medium. The plants rooted on 1/2 strength basal medium supplemented with 1.1 μM indole-3-acetic acid and were transferred to the field with 40% survival.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anonymous (1956) In: The Wealth of India, Raw materials. Vol (X); Publication and Information Directorate CSIR (p 93), New Delhi, India

  • Joshi MS & Thengane SR (1996) In vitro propagation of Azadirachta indica A. Juss (neem) by shoot proliferation. Ind. J. Exp. Biol. 34: 480–482

    Google Scholar 

  • Lloyd G & McCown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by the use of shoot tip culture. Proc. Int. Plant Prop. Soc. 30: 421–427

    Google Scholar 

  • Mathew KM & Hariharan M (1990) In vitro multiple shoot regeneration in Syzygium aromaticum. Ann. Bot. 65: 277–279

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Nayar MP & Sastry ARK (1987) Red Data Book of Indian Plants (Vol. 1), Botanical Survey of India (p 217), Calcutta, India

    Google Scholar 

  • Rahman SM, Hossain M, Biswas BK, Joarder OI & Islam R (1993) Micropropagation of Caesalpina pulcherrima through nodal bud culture of mature tree. Plant Cell Tiss. Org. Cult. 32: 363–365

    Google Scholar 

  • Suparman U & Blake J (1990) Studies on tissue culture of clove tree plant. Indonesian J. Crop Sci. 5: 67–75

    Google Scholar 

  • Yadav U, Lal M & Jaiswal VS (1990) In vitro micropropagation of the tropical fruit tree Syzygium cuminii L. Plant Cell Tiss. Org. Cult. 21: 87–92

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Tissue Culture Department, M.S. Swaminathan Research Foundation, 3rd Cross Street, Taramani Institutional Area, Madras, 600 113, India

    Ajith Anand, C. Srinivasa Rao & P. Balakrishna

Authors
  1. Ajith Anand
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Srinivasa Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Balakrishna
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Anand, A., Srinivasa Rao, C. & Balakrishna, P. In vitro propagation of Syzygium travancoricum Gamble – an endangered tree species. Plant Cell, Tissue and Organ Culture 56, 59–63 (1999). https://doi.org/10.1023/A:1006235900696

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006235900696

Search

Navigation