Abstract
The tsetse, Glossina morsitans morsitans, has two pairs of Malpighian tubules. Ultrastructurally, the tubules consist of three recognizable functional zones: the distal, intermediate and proximal regions, which are characterized by three distinct cell types: I, II and III respectively. The three cell types had numerous microvilli and basal infoldings: the microvilli of type I and II cells were associated with long mitochondria, while those of type III were devoid of mitochondria. Autoradiographic observations using (3H) glucose indicated that there was synthetic activity in the tubules, and that there was greater incorporation of the radiochemical in the distal and intermediate tubule regions than in the proximal region. The distal and intermediate regions have some functional similarities. With regard to primary urine formation in the tubules, it is suggested that the cell types I and II (and hence the distal and intermediate regions) are secretory, and the cell type III (that is, the proximal region) is absorptive. “Canaliculi” in the proximal region increase the surface area for absorption.
Résumé
Le tsétsé, Glossina morsitans morsitans, a deux paires de tubes Malpigiens. Ultra-structurellement, les tubes sont formés de 3 zones de fonction reconnaissable: les régions distale, intermédiaire et proximale caractérisées respectivement par trois types de cellules distinctes: I, II et III. Ces trois types de cellules présentent de nombreux microvilli et des replis basaux. Les microvilli des cellules de type I et II sont associés aux longues mitochondries, alors que ceux du type III en sont dépourvus. Des observations autoradiographiques par utilisation de glucose (3H) indiquent qu’il y a une activité synthétique dans les tubes avec une plus forte incorporation du produit radiochimique dans les régions distale et intermédiaire par rapport à la région proximale. Les régions distale et intermédiaire ont des fonctions similaires. Compte tenu de la formation primaire de l’urine dans les tubes, on peut indiquer que les cellules de type I et II (régions distale et intermédiaire) sont sécrétoires, et que les cellules de type III (région proximale) sont absorbantes. Les “canalicules” dans la région proximale augmentent la surface de la zone d’absorption.
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References
Alberts B., Bray B., Lewis J., Raff M., Roberts K. and Watson J. D. (1983) Molecular Biology of the Cell. Garland Publishing, Inc., New York.
Alkassis W. and Schoeller-Raccaud J. (1984) Ultrastructure of the Malpighian tubules of blowfly larva, Cal-liphora erythrocephala Meigen. (Diptera: Calliphoridae). Int. J. Insect Morphol. Embryol. 13, 215–231.
Berridge M. J. (1966) Metabolic pathways of isolated Malpighian tubules of the blowfly functioning in an artificial medium. J. Insect Physiol. 12, 1523–1538.
Berridge M. J. and Oschman J. L. (1969) A structural basis for fluid secretion by the Malpighian tubules. Tissue & Cell 1, 247–272.
Berridge M. J. and Oschman J. L. (1972) Transporting Epithelia. Academic Press, London.
Bulger R. E. (1983) The urinary system. In Histology, Cell and Tissue Biology (Edited by Weiss L.), pp. 869–913. Elsevier Biomedical, Amsterdam.
Cochran D. G. (1975) Excretion in insects. In Insect Biochemistry and Function (Edited by Candy D. J. and Kilby B. A.),pp. 177–281. Chapman and Hall, London.
Cochran D. G. (1985) Excretory systems. In Fundamentals of Insect Physiology (Edited by Blum M. S.), pp. 59–89. John Wiley and Sons, New York.
Eichelberg D. and Wessing A. (1975) Morphology of the Malpighian tubules of insects. Fortschr. Zool. 23, 124–147.
Fawcet D. (1981) The Cell. Saunders, Philadelphia.
Gee J. D. (1974) Mechanism and control of diuresis in the tsetse fly, Glossina austeni. Ph.D. thesis, University of Cambridge.
Gee J. D. (1975) Diuresis in the tsetse fly, Glossina austeni. J. Exp. Biol. 63, 381–390.
Gee J. D. (1976) Active transport of sodium by the Malpighian tubules of the tsetse fly, Glossina morsitans. J. Exp. Biol. 64, 357–368.
Glauert A. M. (1975) Fixation, dehydration and embedding of biological specimens. In Practical Methods in Electron Microscopy (Edited by Glauert A. M.), North-Holland, London.
Green L. F. B. (1979) Regional specialization in the Malpighian tubules of the New Zealand glow-worm, Arachnocampa luminosa (Diptera: Mycetophilidae). The structure and function of Type I and II cells. Tissue & Cell 11, 673–702.
Maddrell S. H. P. (1971) The mechanisms of insect excretory systems. Adv. Insect Physiol. 8, 199–331.
Maddrell S. H. P. (1978) Physiological discontinuity in an epithelium with an apparently uniform structure. J. Exp. Biol. 75, 133–145.
Maddrell S. H. P. (1980) The control of water relations in insects. In Insect Biology in the Future “VBW 80” (Edited by Locke M. and Smith D. S.),pp. 179–199. Academic Press, London.
Martoja R. and Ballan-Dufrançais C. (1984) The ultra-structure of the digestive and excretory organs. In Insect Ultrastructure (Edited by King R. C and Akai H.) pp. 199–268. Plenum Press, London.
Mcfarlene J. E. (1985) Nutrition and digestive organs. In Fundamentals of Insect Physiology, (Edited by Blum M. S.), pp. 59–89. John Wiley and Sons, New York.
Reynolds E. S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 202–212.
Smith D. S. (1968) Insect Cells: Their Structure and Function. Oliver and Boyd, Edinburg.
Smith D. S. and Littau V. C. (1960) Cellular specialization in the excretory epithelia of an insect, Macrosteles fas-cifrons Stål (Homoptera). J. biophys. biochem. Cytol. 8, 103–133.
Sohal R. S. (1974) Fine structure of the Malpighian tubules in the house fly Musca domestica. Tissue & Cell 6, 719–728.
Wall B. J., Oschman J. L. and Schmidt B. A. (1975) Morphology and function of the Malpighian tubules and associated structures in the cockroach, Periplaneta americana. J. Morphol. 146, 265–306.
Weibel E. R. (1979) Stereologicat Methods: Vol. 1, Practical Methods for Biological Morphometry. Academic Press, London.
Wigglesworth V. B. (1931) The physiology of excretion in a blood-sucking insect, Rhodnius prolixus (Hemiptera, Reduviidae) II: anatomy and histology of the excretory systems. J. exp. Biol. 8, 428–442.
Wigglesworth V. B. and Salpeter M. M. (1962) Histology of the Malpighian tubules in Rhodnius prolixus Stål (Hemiptera). J. Insect Physiol. 8, 299–307.
Williams M. A. (1977) Autoradiography and immunocytochemistry. In Practical Methods in Electron Microscopy (Edited by Glauert A. M.), North-Holland, London.
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Kongoro, J.A., Odhiambo, T.R. Functional Ultrastructure of Malpighian Tubules of Tsetse, Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Int J Trop Insect Sci 9, 563–571 (1988). https://doi.org/10.1017/S1742758400011152
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DOI: https://doi.org/10.1017/S1742758400011152