Abstract
The sex-stimulant contact pheromone present in the cuticle of female tsetse Glossina pallidipes Austen has been extracted with hexane, separated by preparative gas chromatography and identified by mass spectrometry. The aphrodisiac, consisting of 13,17-dimethylpentatriacontane and 15,19-dimethylpentatriacontane, is present in cuticular extracts of 1-week-old females and, in lower amounts, in teneral females but it is almost absent in males of all ages. Full copulatory responses to hexane-washed dead males treated with the two dimethylpentatriacontane isomers were induced in 10–20-day-old adult male G. pallidipes. The practical uses to which this discovery can now be put—towards the mass-rearing and control of G. pallidipes—are discussed in detail.
Similar content being viewed by others
References
Baker R., Bradshaw J. W. S., Evans D. A., Higgs M. D. and Wadhams J. (1976) An efficient all-glass splitter and trapping system for gas chromatography. J. Chromat. Sci. 14, 425–427.
Blomquist G. J., Blailock T. T., Scheetz R. W. and Jackson L. L. (1976) Cuticular lipids of insects — VII. Cuticular hydrocarbons of the crickets Acheta domesticus, Gryllus Pennsylvania and Nemobius fasciatus. Comp. Biochem. Physiol. 54B, 381–386.
Carlson D. A., Langley P. A. and Huyton P. (1978) Sex pheromone of the tsetse fly: isolation, identification, and synthesis of contact aphrodisiacs. Science 201, 750–753.
Challier A. (1965) Amélioration de la méthode de détermination de l’âge physiologique des glossines. Etudes faites sur Glossina palpalis gambiensis Vanderplank, 1949. Bull. Soc. Path. exot. 58, 250–259.
Challier A. (1973) Ecologie de Glossina palpalis gambiensis Vanderplank, 1949 (Diptera: Muscidae) en savane d’Afrique occidentale. Mém. ORSTOM, Paris 64, 274.
Challier A. (1977) Trapping technology. In Tsetse, The Future for Biological Methods in Integrated Control (Ed. by Laird M.), IDRC-077e, Ottawa.
Challier A. and Gouteux J.-P. (1978) Enquêtes entomologiques dan le foyer de maladie du Sommeil de Vavous — Republiques de Côte d’Ivoire (janmar 1978). II. Possibilitiés et essais de lutte en zone forestière contre Glossina palpalis palpalis (R-D). Rapport OCCGE-Centre Muraz, number 20/ENT. 78.
Challier A. and Laveissiere C. (1973) Un nouveau piège pour la capture des glossines (Glossina:Diptera, Muscidae): description et essais sur le terrain. Cah. O.R.S.T.O.M. Sér. Ent. med. Parasit. 11, 252–262.
Harley J. M. N. (1966) Studies on age and trypanosome infection rate in females of Glossina pallidires Aust., G. palpalis fuscipes Newst, and G. brevipalpis Newst, in Uganda. Bull. ent. Res. 57, 23–37.
Howard R. W., McDaniel C. A., Nelson D. R. and Blomquist G. J. (1980) Chemical ionisation mass spectrometry. Application to insect derived cuticular alkanes. J. chem. Ecol. 6, 609–623.
Huyton P. M., Langley P. A., Carlson D. A. and Coates T. W. (1980a) The role of sex pheromones in initiation of copulatory behaviour by male tsetse flies, Glossina morsitans morsitans. Physiol. Ent. 5, 243–252.
Huyton P. M., Langley P. A., Carlson D. A. and Schwarz M. (1980b) Specificity of contact sex pheromones in tsetse flies Glossina spp. Physiol. Ent. 5, 253–264.
Jaenson T. G. T. (1980) Mating behaviour of females of Glossina pallidipes Austen (Diptera:Glossinidae). Bull. ent. Res. 70, 49–60.
Jordan A. M. (1958) The mating behaviour of females of Glossina palpalis (R-D) in captivity. Bull. ent. Res. 49, 35–43.
Kovats E. (1965) Gas chromatographic characterization of organic substances and the retention index system. In Advances in Chromatography (Ed. by Giddings J. C.), Vol. 1, Chap. 7, pp. 229–247.
Langley P. A. (1977) Physiology of tsetse flies (Glossina spp.) (Diptera: Glossinidae): a review. Bull. ent. Res. 67, 527–574.
Langley P. A., Carlson D. A. Huyton P. M. and Coates T. W. (1979) Prospects for use of specific sex pheromones for tsetse control. 16th Meeting of International Scientific Council for Trypanosomiasis Research and Control, Yaounde, Cameroun.
Langley P. A., Pimley R. W. and Carlson D. A. (1975) Sex recognition pheromone in tsetse fly Glossina morsitans. Nature, Lond. 254, 51–53.
Nash T. A. M., Jordan A. M. and Trewern M. A. (1971) Mass rearing of tsetse flies (Glossina spp.): present advances. In Sterility Principle for Insect Control or Eradication (publication No. sII/PUB/265), pp. 99–110. International Atomic Energy Agency, Vienna.
Okiwelu S. N. (1977) Insemination, pregnancy and suspected abortion rates in a natural population of Glossina morsitans morsituns (Diptera: Glossinidae) in the Republic of Zambia. J. med. Ent. 14, 19–23.
Rogers A. (1972) Studies on the mating of Glossina palldipes Austen. I. The age at mating. Ann. trap. Med. Parasit. 66, 515–523.
Saunders D. S. (1960) Ovulation cycle in Glossina morsitans Westwood (Diptera:Muscidae) and a possible method of age determination for female tsetse flies by the examination of their ovaries. Trans. R. ent. Soc. Lond. 112, 221–238.
Saunders D. S. (1962) Age determination for female tsetse flies and the age compositions of samples of Glossina pallidipes (Aust., G. palpalis fuscipes Newst and G. brevi-palpis Newst. Bull. ent. Res. 53, 579–595.
Vale G. A. and Hargrove J. W. (1975) Field attraction of tsetse flies (Diptera:Glossinidae) to ox odour; the effect of dose. Proc. Trans. Rhod. scient. Ass. 56, 46–50.
van Etten J. (1981) A comparison of the performance of laboratory colonies of Glossina pallidipes Austen from two allotropic populations in Kenya. Insect Sci. Applic. 1, 177–183.
Whitehead D. L. (1981) The effect of phytosterols on tsetse reproduction. Insect Sci. Applic. 1, 281–288.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McDowell, P.G., Whitehead, D.L., Chaudhury, M.F.B. et al. The Isolation and Identification of the Cuticular Sex-Stimulant Pheromone of the Tsetse Glossina Pallidipes Austen (Diptera: Glossinidae). Int J Trop Insect Sci 2, 181–187 (1981). https://doi.org/10.1017/S1742758400000990
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1017/S1742758400000990