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Neuronal nitric oxide synthase has a role in the detrimental effects of lipopolysaccharide on spatial memory and synaptic plasticity in rats

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Abstract

Background

The role of neuronal nitric oxide synthase (nNOS) in lipopolysaccharide (LPS)-induced memory and synaptic plasticity impairment was investigated.

Methods

The rats were divided and treated as follows: (1) control (saline), (2) LPS, (3) 7NI (7-nitroindazole as a nNOS inhibitor)-LPS and (4) 7NI.

Results

In a Morris water maze, the LPS group took a longer amount of time and traveled a greater distance to reach the platform, this was prevented by 7NI. Malondialdehyde (MDA) and nitric oxide (NO) metabolites in the hippocampus of the LPS group were higher while the total thiol, superoxide dismutase and catalase were lower than that of the controlled specimen. Pre-treatment using 7NI prevented the changes in the biochemical criteria. The slope and amplitude of the field excitatory post-synaptic potential (fEPSP) in the LPS group decreased, whereas in 7NI-LPS group they increased.

Conclusion

It is suggested that inhibition of nNOS by 7NI improves the deleterious effects of LPS by reducing NO metabolites and the brain tissues oxidative damage.

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Authors and Affiliations

  1. Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Akbar Anaeigoudari, Farimah Beheshti & Mahmoud Hosseini

  2. Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Soukhtanloo

  3. Neurogenic Inflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Naser Shafei, Sepehr Behradnia & Seyed Mojtaba Mousavi

  4. Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Hamid Reza Sadeghnia

  5. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

    Parham Reisi

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  1. Akbar Anaeigoudari
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Correspondence to Mahmoud Hosseini.

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Anaeigoudari, A., Soukhtanloo, M., Shafei, M.N. et al. Neuronal nitric oxide synthase has a role in the detrimental effects of lipopolysaccharide on spatial memory and synaptic plasticity in rats. Pharmacol. Rep 68, 243–249 (2016). https://doi.org/10.1016/j.pharep.2015.09.004

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  • DOI: https://doi.org/10.1016/j.pharep.2015.09.004

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