Abstract
We investigated the influence of peptide size on the apparent loss of sequence during collision-induced dissociation (CID) of b ions using a group of peptides containing from between 4 and 10 residues. Although scrambling of sequence for b +3 generated from tetrapeptides is minimal, significant formation of nondirect sequence ions (i.e., ions for which scrambling has apparently occurred) was observed for all larger b ions included in the study.
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Published online August 12, 2009
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Molesworth, S., Osburn, S. & Van Stipdonk, M. Influence of size on apparent scrambling of sequence during CID of b-type ions. J Am Soc Mass Spectrom 20, 2174–2181 (2009). https://doi.org/10.1016/j.jasms.2009.07.023
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DOI: https://doi.org/10.1016/j.jasms.2009.07.023