Abstract
Echinodorus scaber Rataj and Echinodorus grandiflorus (Cham. & Schltdl.) Micheli, Alismataceae, are popularly used to relieve inflammatory complaints and as diuretic. A study on the antinociceptive effect and selected marker compounds in eleven extracts from different locations was undertaken and their antinociceptive effect was assessed. The fingerprints were compared by HPLC-DAD and the content of vitexin, isovitexin, isoorientin and vitexin-2-O-rhamnoside were determined. All samples presented antinociceptive activity reducing the writhes by 36.4–62.5% and 47.4–79.8% at 10 and 50 mg/kg, respectively; indomethacin (5 mg/kg) reduced writhes by 82.6–90.1%. The content of the flavonoids C-glycosides, however, presented a strong variation. Isovitexin and isoorientin were found in all the samples, with content ranging from traces to 14.70 μg/mg and 2.12–84.27 μg/mg extract, respectively, while vitexin-2-O-rhamnoside occurred in quantifiable amounts only in 3 out of 11 samples ranging from 5.43 to 33.13 μg/mg extract; vitexin was not detected at all or detected in trace amounts. According to the fingerprints, the samples could be arranged in four main groups. All eleven extracts showed antinociceptive activity. Isovitexin was the only flavonoid present in all samples and can be regarded, acting in synergy with the other compounds or not, as the responsible for the antinociceptive activity. Therefore, isovitexin is a good choice as chemical marker when the antinociceptive activity of E. scaber and E. grandiflorus is investigated.
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CLE, KCL and ELD participated in phytochemical analysis. VCS participated in interpretation of NMR spectral data. EFGCD participated in HPLC analysis,acquisition and interpretation of data. RVR and DTOM participated in the evaluation of antinociceptiveactivity, acquisition and interpretation of data. CAC participated in PCA analysis,acquisition and interpretation of data. PTSJ authored the project and supervised its execution and the drafting of this paper and GSH participated in the discussion of the data presented herein as well as the drafting of the manuscript.
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Strada, C.L., da Lima, K.C., da Silva, V.C. et al. Isovitexin as marker and bioactive compound in the antinociceptive activity of the Brazilian crude drug extracts of Echinodorus scaber and E. grandiflorus. Rev. Bras. Farmacogn. 27, 619–626 (2017). https://doi.org/10.1016/j.bjp.2017.05.011
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DOI: https://doi.org/10.1016/j.bjp.2017.05.011