Abstract
The kinetics of excretion of the novel tranquilizer cinazepam (3-hydroxy-7-bromo-5-(ortho-chlorophenyl)-1,2-dihydro-3H-1,4-benzdiazepin-2-one hemisuccinate (I)) in mice after a single administration and different schemes of multiple administration were determined. Mass balance was studied daily in excretions of mice (feces and urine) for 5–10 days. We observed that monoexponen-tial renal excretion of 14C-cinazepam and its metabolites predominated with all dosage regimens. Cinazepam and its metabolites were almost fully (> 90%) eliminated in urine and feces over the period of study (5–10 days), which means that no significant accu-mulation of the drug in the body occurred. The kinetic parameters of drug excretion were not significantly different after a single in-jection compared with those following multiple doses of 14C-cinazepam administration. This finding suggests the absence of induction (repression) of enzymatic systems after multiple administration and lack of influence on the kinetic scheme of cinazepam elimination from mice.
In our work, we also presented a modification of the Mansgeldorf’s method for analysis of kinetic parameters during multiple administration of the tranquilizer. We demonstrated that our modified approach could be equally and efficiently applied for interpreting experimental data during a single dose administration and after chronic administration of xenobiotics. The use of this method made it possible to evaluate the relative efficiency of elimination processes and to find current values for excretion constants during sampling intervals.
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Andronati SA, Yakubovskaya LN, Yavorsky AS, Pavlovski VI, Golovenko MYa, Seredenin SB, Voronina TA et al.: Hemisuccinate - 3-hydroxy-7-bromo-5 - (ortho-chloro)-phenyl-1.2-dihydro-3H -1,4-benzdiaze-pin-2-one, which has a hypnotic, sedative, tranquilizing activity. Patent 19803, Ukraine MKI 5CO7 D 243/14, A 61 K31/55. od 25.12.1997. Bull. 6, 18s.
Andronati SA, Karasyova TL, Popova KI, Makan SYu, Boyko IA: GABA-ergic hypnotics (Russian). Bulletin of Psychiatry and Psychopharmacotherapy, 2004, 1, 6–17.
Blednov IuA, Kosach IV, Seredenin SB: The effect of 1,4-benzodiazepine derivatives on 35S-tetrabutylbicyclo-phosphorothionate binding in the brain of inbred mice with differing emotional stress reactions (Russian). Eksp Klin Farmakol, 1997, 60, 3–6.
Boyko IA, Makan SYu, Smulsky SP, Andronati SA: The study of affinity for the benzodiazepine sites of GABA A receptors of the CNS and functional activity of potential hypnotic - cinazepam (Russian). Bull of the Odessa Nat Univ, 2005, 10, 49–58.
Cornish-Bowden A: Fundamentals of Enzyme Kinetics, Portland Press Ltd., London, 2004.
Danneberg P, Weber KH: Chemical structure and biological activity of the diazepines. Br J Clin Pharmacol, 1983, 16, Suppl 2, 244.
Davies M, Newell JG, Derry JMC, Martin IL, Dunn SMJ: Characterization of the interaction of zopiclone with ψ-aminobutyric acid type-A receptors. Mol Pharmacol, 2000, 58, 756–762.
Dingemanse J, Voskuyl RA, Langemeijer MW, Postel-Westra I, Breimer DD, Meinardi H, Danhof M: Pharmacokinetic-pharmacodynamic modelling of the anticonvulsant effect of oxazepam in individual rats. Br J Pharmacol, 1990, 99, 53–58.
Dujardin K, Guieu JD, Leconte-Lambert C, Leconte P, Borderies P, de La Giclais B:. Comparison of the effects of zolpidem and flunitrazepam on sleep structure and daytime cognitive functions. A study of untreated unsomniacs. Pharmacopsychiatry, 1998, 31, 14–18.
Ferentinos P, Paparrigopoulo T: Zopiclone and sleepwalking. Int J Neuropsychopharmacol, 2009, 12, 141–142.
Godlevsky LS, Karasyova TL, Popova KI, Andronati SA: Influence of cinazepam on sleep-wake up cycle in rats (Russian). Progress of Biology and Medicine, 2005, 2, 22–26.
Gottesmann C: Detection of seven sleep-waking stages in the rat. Neurosci Biobehav Rev, 1992, 16, 31–38.
Gottesmann C, Gandolfo G, Arnaud C, Gauthier P: The intermediate stage and paradoxical sleep in the rat: influ-ence of three generations of hypnotics. Eur J Neurosci, 1998, 10, 409–414.
Guentert TW: Time-dependence in benzodiazepine pharmacokinetics. Mechanisms and clinical significance. Clin Pharmacokinet, 1984, 9, 203–210.
Karaseva TL, Popova LV, Onufrienko OV, Andronati KS, Andronati SA: Experimental study of the development of tolerance to cinazepam (Russian). Reports of NAN of Sciences of Ukraine, 2008, 7, 157–161.
Keleti T: Basic enzyme kinetics, Akademiai Kiado, Budapest, 1986.
Lasoń W, Dudra-Jastrzebska M, Rejdak S, Czuczwar J: Basic mechanisms of antiepileptic drugs and their pharmacokinetic/pharmacodynamic interactions: an update. Pharmacol Rep, 2011, 63, 271–292.
Makan SY, Boiko I, Smulskii SP, Andronati CA: Effect of cinazepam administration on the ligand affinity of neuromediator system receptors in rat brain. Pharm Chem J, 2007, 41, 249–252.
Maykova AV, Onofriychuk AA, Sulakova OB, Pavlichenko OD: Determination of anesthetic cinazepam properties in the rat (Russian). Bull Odessa Nat Univ, 2006, 11, 228–234.
Maykova AV, Zhuk OV: Anticonvulsant activity of cinazepam on the model seizures with different mechanism of forming (Russian). Bull Odessa Nat Univ, 2005, 10, 165–175.
Nachkebia N, Dzadzamia Sh, Chkhartishvili E, Mchedlidze O, Oniani T: Influence of diazepam on different behavioral states of sleep-waking cycle. Georgian Med News, 2009, 4, 94–99.
Onishi S, Kawade N, Itoh S, Isobe K, Sugiyama S, Hashimoto T, Narita H: Kinetics of biliary excretion of the main two bilirubin photoproducts after injection into Gunn rats. Biochem J, 1981, 198, 107–112.
Schukin SI, Zinkovsky VG: Biotransformation and biokinetics of the cinazepam - novel drugs possessing psychotropic activity. Abstracts of Joint Meeting on Medicinal Chemistry. Kraków, 2003, 60.
Sivertsen B, Omvik S, Pallesen S, Nordhus IH, Bjorvatn B: Sleep and sleep disorders in chronic users of zopiclone and drug-free insomniacs. J Clin Sleep Med, 2009, 5, 349–354.
Tarka SM Jr, Arnaud MJ, Dvorchik BH, Vesell ES: Theobromine kinetics and metabolic disposition. Clin Pharmacol Ther, 1983, 34, 546–555.
Varfolomeev SD, Gurevich KG: Biokinetics: Practical course, Fairpress, Moscow, 1999.
Welling PG: Graphic methods in pharmacokinetics: the basics. J Clin Pharmacol. 1986, 26, 510–514.
Zhuk OV, Zinkovsky VG, Schukin SI: Kinetic analysis of the process elimination after single and different schemes of multiple administration of cinazepam — a novel prodrug possessing psychotropic activity. Eur Neuropsychopharmacol, 2005, 15, Suppl 2, 155–156.
Zhuk OV, Zinkovsky VG, Schukin SI, Sivachenko AV: Biotransformation, pharmacokinetics and pharmacody-namics of cinazepam. Pharmacol Rep, 2007, 59, Suppl 1, 60–61.
Zin’kovskii VG, Vasilinin GB, Stankevich EA, Golovenko NYa, Zhuk OV: Pharmacokinetics of bromonordiazepam and its 14C- analog in single and prolonged patterns of administration. Pharmaceut Chem J, 1988, 22, 197–204.
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Schukin, S.I., Zinkovsky, V.G. & Zhuk, O.V. Elimination kinetics of the novel prodrug cinazepam possessing psychotropic activity in mice. Pharmacol. Rep 63, 1093–1100 (2011). https://doi.org/10.1016/S1734-1140(11)70628-4
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DOI: https://doi.org/10.1016/S1734-1140(11)70628-4