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Biomimetic Synthesis of Collagen/Nano-Hydroxyapitate Scaffold for Tissue Engineering

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Abstract

This paper reports a precipitation method for the fabrication of compositionally graded biomimetic collagen/nano-hydroxyapatite (HA) composite scaffold. The method is centrifugation based and produce the precipitation of nano-HA crystallites in situ (calcium ions (Ca2+) react phosphate ions (PO43−) and precipitate a non-stoichiometric hydroxyapatite). It was observed that prism needle-like nano-HA crystallites (about 2.5 nm × 3 nm× 25 nm) precipitated on collagen fibrils in the interior of collagen matrix. Chemical and microstructure analysis revealed a gradient of the Ca to P ratio across the width of the scaffold, lead to the formation of a HA-rich side and a HA-deplete side of scaffold. The HA-rich side featured low porosity and agglomerates of the nano-HA crystallites; while HA-depleted side featured higher porosity and nano-HA crystallites integrated with collagen fibrils to form a porous network structure.

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  1. Advanced Materials & Biomaterials Research Centre, School of Engineering, The Robert Gordon University, School Hill, Aberdeen, AB10 1FR, UK

    Chao-zong Liu

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  1. Chao-zong Liu
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Liu, Cz. Biomimetic Synthesis of Collagen/Nano-Hydroxyapitate Scaffold for Tissue Engineering. J Bionic Eng 5 (Suppl 1), 1–8 (2008). https://doi.org/10.1016/S1672-6529(08)60064-5

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  • DOI: https://doi.org/10.1016/S1672-6529(08)60064-5

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