Abstract
Our aim was to determine the mechanisms by which intraileal fat alters proximal gastrointestinal motility—the ileal brake. Five mongrel dogs with ileal Thiry-Vella fistulas were equipped with strain gauge force transducers on the upper gut to measure contractile activity. Ileal infusions of 115 mmol/L oleic acid and triglyceride were studied in dogs with extrinsically innervated and extrinsically denervated Thiry-Vella loops. Plasma concentrations of peptide YY and total glucagon-like immunoactivity were measured. Oleic acid but not triglyceride inhibited postprandial contractions in the gastric antrum in dogs with innervated and denervated Tbiry-Vella loops. Postprandial duodenal and jejunal motility was inhibited by oleic acid regardless of extrinsic denervation to the loops (P <0.05), but triglyceride inhibited small intestinal motility only in dogs with innervated Thiry-Vella loops. Intraileal oleic acid but not triglyceride increased plasma concentrations of peptide YY and total glucagon-like immunoactivity in dogs with innervated and denervated Thiry-Vella loops. Intraileal oleic acid inhibits gastric and small intestinal motility possibly via increased plasma concentrations of peptide YY and enteroglucagon. Intact extrinsic innervation is necessary for intraileal triglyceride to inhibit small intestinal motility.
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References
Spiller RC, Trotman IF, Higgins BE, Ghatei MA, Grimble GK, Lee YC, Bloom SR, Misiewicz JJ, Silk DB. The ileal brake—inhibition of jejunal motility after ileal fat perfusion in man. Gut 1984;25:365–374.
Spiller RC, Trotman IF, Adrian TE, Bloom SR, Misiewicz JJ, Silk DB. Further characterisation of the ‘ileal brake’ reflex in man—effect of ileal infusion of partial digests of fat, protein, and starch on jejunal motility and release of neurotensin, enteroglucagon, and peptide YY. Gut 1988;29:1042–1051.
Siegle ML, Schmid HR, Ehrlein HJ. Effects of ileal infusions of nutrients on motor patterns of canine small intestine. Am J Physiol 1990;259:G78-G85.
Pironi L, Stanghellini V; Miglioli M, Corinaldesi R, Giorgio RD, Ruggeri E, Tbsetti C, Poggioli G, Labate AMM, Monetti N, Gozzetti G, Barbara L, Go, VLW. Fat-induced ileal brake in humans: A dose-dependent phenomenon correlated to plasma levels of peptide YY. Gastroenterology 1993;105:733–739.
Pappas TN, Debas HT, Chang AM, Taylor IL. Peptide YY release by fatty acids is sufficient to inhibit gastric emptying in dogs. Gastroenterology 1986;91:1386–1389.
Read NW, McFarlane A, Kinsman RI, Bates TE, Blackball NW, Farrar GBJ, Hall JC, Moss G, Morris AP, O’Neill B, Welch I, Lee Y, Bloom SR. Effect of infusion of nutrient solutions into the ileum on gastrointestinal transit and plasma levels of neurotensin and enteroglucagon. Gastroenterology 1984;86:274–280.
Brown NJ, Richards WJ. Involvement of extrinsic innervation in the ‘ileal brake’ mechanism. J Gastrointest Motil 1991;3:175.
Read NW, Welch IMcL. Naloxone prevents the effect of ileal lipid on small bowel transit but not on gastric emptying. Gut 1984;25:A1326.
Brown NJ, Rumsey RDE, Bogentoft C, Read NW. The effect of adrenoceptor antagonist on the ileal brake mechanism in the rat. Br J Pharmacol 1992;105:751–755.
Brown NJ, Horton A, Rumsey RDE, Read NW. Granisetron and ondansetron: Effects on the ileal brake mechanism in the rat. J Pharm Pharmacol 1993;45:521–524.
Brown NJ, Rumsey RDE, Read NW. The effect of the cholecystokinin antagonist devazepide (L364718) on the ileal brake mechanism in the rat. J Pharm Pharmacol 1993;45:1033–1036.
Ohneda A, Nihei J, Kobayashi T, Umetz M, Sasaki T, Sanoyama K. Effect of insulin treatment upon response of extrapancreatic glucagon on arginine. Tohoku J Exp Med 1979; 129:207–217.
Wen J, Phillips SF, Sarr MG, Kost LJ, Holst JJ. PYY and GLP-1 contribute to feedback inhibition from the canine ileum and colon. Am J Physiol 1995;269:G945-G952.
Ohtani N, Sasaki I, Naito H, Shibata C, Funayama Y, Kamiyama Y, Takahashi M, Matsuno S. Inhibitory effect on peptide YY, neurotensin and glicentin on upper gastrointestinal motility in dogs. BiomedRes 1994;lS(Supplement 2):299–302.
Tbda M, Sasaki I, Naito H, Funayama Y, Kamiyama Y, Suzuki Y, Takahashi M, Matsuno S, Ohneda A, Igarashi H. Effect of ileo-jejunal transposition on intestinal structure and function in dogs. Biomed Res 1988;9(Supplement 3):1.57–162.
Sarr MG, Duenes JA. Early and long term effects of a model of intestinal autotransplantation on intestinal motor patterns. Surg Gynecol Obstet 1990;170:338–346.
Lin HC, Zhao XT, Wang LJ. Intestinal transit is more potently inhibited by fat in the distal (ileal brake) than in the proximal (jejunal brake) gut. Dig Dis Sci 1997;42:19–25.
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Ohtani, N., Sasaki, I., Naito, H. et al. Mediators for fat-induced ileal brake are different between stomach and proximal small intestine in conscious dogs. J Gastrointest Surg 5, 377–382 (2001). https://doi.org/10.1016/S1091-255X(01)80065-2
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DOI: https://doi.org/10.1016/S1091-255X(01)80065-2