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Matrix-assisted laser desorption/ionization Fourier transform mass spectrometry of luteinizing hormone releasing hormone-metal ion complexes

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Journal of the American Society for Mass Spectrometry

Abstract

Complexes of luteinizing hormone releasing hormone (LHRH) with divalent metal ions (Ni, Zn, Cu) have been studied by matrix-assisted laser desorption ionization (MALDI) and Fourier transform mass spectrometry. LHRH-metal complexes were detected in high abundance for all three metals from synthesized samples, particularly in negative ion mode. The mixture of the apopeptide with the metal salts yielded in most cases a very minor signal of metal-complex ions. As opposed to Ni and Zn, copper complex ions were mostly observed as Cu(I) adducts. This can be partly attributed to plume reactions of Cu(I) with the apopeptide. the Cu(II) complexes appeared only for the synthetic complex. We show how to distinguish between the contribution to the overall signal from desorbed complexes and from Cu(I) complexes formed in the MALDI plume.

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Author notes

  1. Bekir Salih

    Present address: Dept of Chemistry, Hacettape University, 06532, Ankara, Turkey

Authors and Affiliations

  1. Department of Chemistry, Swiss Federal Institute of Technology (ETH), Universitatsstr 16, CH-8092, Zurich, Switzerland

    Christophe Masselon, Bekir Salih & Renato Zenobi

Authors
  1. Christophe Masselon
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  2. Bekir Salih
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  3. Renato Zenobi
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Correspondence to Renato Zenobi.

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Masselon, C., Salih, B. & Zenobi, R. Matrix-assisted laser desorption/ionization Fourier transform mass spectrometry of luteinizing hormone releasing hormone-metal ion complexes. J Am Soc Mass Spectrom 10, 19–26 (1999). https://doi.org/10.1016/S1044-0305(98)00128-7

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  • DOI: https://doi.org/10.1016/S1044-0305(98)00128-7

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