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Rapidly switchable matrix-assisted laser desorption/ionization and electrospray quadrupole-time-of-flight mass spectrometry for protein identification

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Journal of the American Society for Mass Spectrometry

Abstract

We describe a new interface for a prototype quadrupole—quadrupole-time-of-flight (TOF) mass spectrometer (Centaur, Sciex) that allows rapid switching between electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) modes of operation. Instrument performance in both modes is comparable (i.e., resolution ∼10,000 FWHM, mass accuracy <10 ppm, sensitivity ∼1 fmol) because the ion source is decoupled from the TOF mass analyzer by extensive gas collisions in the quadrupole stages of the instrument. The capacity to obtain side-by-side high quality ESI and MALDI mass spectra from a single proteolytic mixture greatly facilitates the identification of proteins and elucidation of their primary structures. Improved strategies for protein identification result from this ability to measure spectra using both ionization modes in the same instrument and to perform MS/MS on singly charged as well as multiply charged ions. Examples are provided to demonstrate the utility and performance of the modified instrument.

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Authors and Affiliations

  1. Rockefeller University, 1230 York Avenue, 10021, New York, NY

    Andrew N. Krutchinsky, Wenzhu Zhang & Brian T. Chait

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  1. Andrew N. Krutchinsky
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  2. Wenzhu Zhang
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  3. Brian T. Chait
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Correspondence to Brian T. Chait.

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Krutchinsky, A.N., Zhang, W. & Chait, B.T. Rapidly switchable matrix-assisted laser desorption/ionization and electrospray quadrupole-time-of-flight mass spectrometry for protein identification. J Am Soc Mass Spectrom 11, 493–504 (2000). https://doi.org/10.1016/S1044-0305(00)00114-8

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  • DOI: https://doi.org/10.1016/S1044-0305(00)00114-8

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