Abstract
A microwave-powered chemical reaction interface has been installed in a Hewlett-Packard gas chromatograph-mass spectrometer (GC-MS) system (5890 IT gas chromatograph-S971 mass-selective detector). The technical details and optimization strategies are discussed. The evaluation of this new setup is presented, showing detection limits of 1 ng of 13C-, 15N-, and Cl-containing compounds with signal-to-noise ratios greater than or egual to 3. Selective detection was evaluated with a urine sample from a dog dosed with 15N3-midazolam that had been previously analyzed by using a differentially pumped research-level quadrupole mass spectrometer. The results show that the detection of 15N and Cl remains highly selective and the mass-selective detector gives comparable sensitivity to the larger instrument when the latter is operating over a conventional mass range. The capability for chemical reaction interface mass spectrometry can be easily accomplished with an inexpensive GC-MS system.
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Song, H., Kusmierz, J., Abramson, F. et al. Implementation of the chemical reaction interface mass spectrometry technique on a Hewlett-Packard mass-selective detector. J Am Soc Mass Spectrom 5, 765–771 (1994). https://doi.org/10.1016/1044-0305(94)80009-X
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DOI: https://doi.org/10.1016/1044-0305(94)80009-X