Abstract
The 4-POBN[α-(4-pyridyl-l-oxide)-N-tert-butyl-nitrone] radical adducts of ethyl and pentyl radicals were determined by a combination of high performance liquid chromatography (HPLC) combined with electron paramagnetic resonance (EPR) with HPLC-electrospray (ESI)-mass spectrometry and HPLC-thermospray (TSP)-MS. The identifIcation of the peak corresponding to the spin-trapped radical was done by performing HPLC-EPR under the same chromatographic conditions as the HPLC-MS. The radical adducts could be determined by both techniques, even though for ESI only 12 μL/min of the total 1 mL/min HPLC flow rate could be directed into the ion source.
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Parker, C.E., Iwahashi, H. & Tomer, K.B. Spin-trapped Radicals: Determination by LC-TSP-MS and LC-ESI-MS. J Am Soc Mass Spectrom 2, 413–418 (1991). https://doi.org/10.1016/1044-0305(91)85007-S
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DOI: https://doi.org/10.1016/1044-0305(91)85007-S