Abstract
Several acylating reagents are synthesized and used to introduce quatemary phosphonium or ammonium or ternary sulfonium functions into a simple model of a peptido leukotriene (PLT). One of these reagents was selected for further study with LTE4, LTD4, and LTC4. We demonstrate that acylation of the free amine function of PLTs to produce the 5-triphenylphosphoniumvaleryl-amide (TPPV) derivatives enhances chemical stabilities and significantly increases responses in fast-atom bombardment and continuous-flow liquid secondary ion mass spectrometry (CF-LSIMS) relative to the native PLTs. With high-performance liquid chromatography inlet to CF-LSIMS, we demonstrate the facile detection in selected ion monitoring of the TPPV derivative of 3 pg of LTD4.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Balazy, M.; Murphy, R. C. Anal. Chem. 1986, 58, 1098–1101.
Caprioli, R. R.; Fan, T.; Cottrell, J, S. Anal. Chem. 1986, 58, 2949–2954.
Raftery, M. J.: Thorne, G. C.; Orkiszewski, R. S.; Gaskell, S. Biomed. Environ. Mass Spectrom. 1990, 19, 465–474.
Sala, A.; Kayganich, K.; Zirolli, J. A.; Murphy, R. C. J. Am. Soc. Mass Spectrom. 1991, 2, 314–321.
Kikawa, Y.; Nakai, A.; Shigematsu, Y.; Sudo, M.; Umeda, Y. Nippon lyo Masu Supekutoru Gakkai Koenshu 1992, 17, 155–158.
Deterding, L. J.; Curtis, J. F.; Tomer, K. B. Bio. Mass Spectrom. 1992, 21, 597–609.
Montgomery, J. A.; Mamer, O. A. Anal. Biockem. 1989, 176, 85–95.
Millington, D. S.; Norwood, D. L.; Kodo, N.; Roe, C. R.; Inoue, F. Anal. Biochem. 1989, 180, 331–339.
Chang, Y.-S.; Watson, J. T. J. Am. Soc. Mass Spectrom. 1992, 3, 769–775.
Wagner, D. S.; Salari, A.; Gage, D. A.; Leykam, J.; Fetter, J.; Hollingsworth, R.; Watson, J. T. Bio. Mass Spectrom. 1991, 20, 419–425.
Morris, H. R.; Taylor, G. W. Prostaglandins 1980, 29, 185.
Li, C. S.; Péville, P.; Watson, S.; Mamer, O. A.; Just, G.; Yergey, J. A.; Young, R. J. Chem. Soc., Perkin Trans. 1, submitted.
Tagari, P.; Ethier, D.; Carry, M.; Corley, V.; Charleson, S.; Girard, Y.: Zamboni, R. Clin. Chem. 1989, 35, 388–391.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mamer, O.A., Just, G., Li, CS. et al. Enhancement of mass spectrometric detection of LTC4, LTD4, and LTE4 by derivatization. J Am Soc Spectrom 5, 292–298 (1994). https://doi.org/10.1016/1044-0305(94)85019-4
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1016/1044-0305(94)85019-4