Abstract
The wax layer of apple leaves plays an important role in improving stress resistance, but relatively little is known about the mechanisms of wax synthesis and transport in apple leaves. In this study, 17 wax components, including alcohols, alkanes, fatty acids and terpenes, were analyzed by gas chromatography-tandem mass spectrometry (GC-MS) from the leaves of 123 apple germplasms. Whole-genome sequencing of these apple accessions yielded 5.9 million high-quality single nucleotide polymorphisms (SNPs). We performed a genome-wide association study (GWAS) on 17 wax components and identified several genes related to wax synthesis and transport, including MdSHN1 (SHINE1), MdLTP4 (LIPID TRANSFER PROTEIN4), MdWSD1 (WAX ESTER SYNTHASE/ACYL-COA DIAC-YLGLYCEROL ACYLTRANSFERASE1), MdRDR1 (RNA-DEPENDENT RNA POLYMERASE1), MdACBP6 (ACYL-COA-BINDING PROTEIN6), MdNLE (NOTCHLESS) and MdABCG21 (ATP-BINDING CASSETTE G21). Moreover, we identified some prominent SNPs that may affect gene expression and protein function. These results provide insights into mechanisms of wax synthesis and transport in apple leaves and broaden the genetic resources and basis for facilitating resistance breeding.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Apples contain a wide range of antioxidants and have an overall high nutritional value, making them beneficial for human health (Boyer and Liu 2004). Global apple production in 2018 exceeded 86 million tons (FAOSTAT, http://www.fao.org/faostat/). However, biotic and abiotic stressors that commonly exist in apple production regions often severely impact the yield and quality of apples (Mittelberger et al. 2017; Song et al. 2019).
In order to deal with these stressors, a layer of lipid, called the cuticle, covers the epidermis of the aboveground parts of plants. The cuticle is secreted by epidermal cells and is widely found in stems, leaves, flowers, fruits and other tissues (Buschhaus and Jetter 2011; Yeats and Rose 2013). Wax is an important component of the plant cuticle. According to previous reports, the wax of leaves improves plant drought resistance by preventing the loss of non-stomatal water and plays an important role in cold resistance (Kerstiens 2006; Kunst and Samuels 2003; Liao et al. 2014). In addition, Wax improves plant disease resistance by preventing direct contact with bacteria (Solovchenko and Merzlyak 2003; Yeats and Rose 2013). In plant reproductive organs, wax also plays an important role in preventing genital fusion (Aarts et al. 1995).
Especially in the arid regions in Northwest China, the most important factor affecting plant growth is drought stress, which can lead to decreases of crop yields (Zhang et al. 2015). Rice and wheat plants with higher wax content have stronger drought tolerance and higher yields under drought stress than those with lower wax content (Guo et al. 2016; Zhou et al. 2013). The composition of the wax itself can also impact drought resistance. Alkanes account for 93% of the plant cuticle wax under drought, and increases of wax that occur under drought are mainly due to increases of long-chain alkanes, especially C29, C31 and C33 (Kosma et al. 2009). Similarly, previous studies have shown that a decrease of C29 alkane in cuticle wax reduces drought tolerance (Panikashvili et al. 2007).
Wax is usually composed of very long chain fatty acids (VLCFAs) and their derivatives, including primary alcohols, secondary alcohols, ketones, aldehydes, esters and alkanes (Li-Beisson et al. 2013; Pollard et al. 2008; Samuels et al. 2008). A complex series of enzymatic processes leads to the synthesis of the very-long-chain fatty acids (VLCFAs) that constitute plant epidermal wax. In plastids, malonyl-acyl carrier protein (malonyl-ACP) is a donor of carbon atoms for chain elongation, and the fatty acid synthase (FAS) complex catalyzes the combinations of acetyl-CoA and malonyl-ACP into C16 and C18 long-chain fatty acids, including palmitic acid (16:0), stearic acid (18:0) and oleic acid (18:1) (Li-Beisson et al. 2013; Shepherd and Wynne Griffiths 2006; Troncoso-Ponce et al. 2016; Wang and Benning 2012). The resulting C16 and C18 acyl ACPs are utilized in plastids by acyl ACP thiolase to form the corresponding fatty acid, which is used by long-chain acyl coenzyme A synthetase (LACS) to form acyl CoA (Dormann et al. 1995; Jones et al. 1995; Lu et al. 2009).
Acyl CoA is subsequently transported to the endoplasmic reticulum where it acts as a receptor for further chain extension. Acetyl CoA is utilized by acetyl-CoA carboxylase (ACC) 1 to form malonyl CoA, which is the carbon donor for the chain extension (Lu et al. 2011). The carbon chain is extended under the catalysis of the fatty acid elongase (FAE) complex located in the endoplasmic reticulum. FAE contains four enzymes, β-ketoacyl-CoA synthase (KCS), β-ketoacyl-CoA reductase (KCR), β-hydroxyacyl-CoA dehydratase (HCD) and enoyl-CoA reductase (ECR) (Bach et al. 2008; Beaudoin et al. 2009; Kim et al. 2013; Zheng et al. 2005). These four reactions constitute a cycle in the extension process of VLCFAs, in which each cycle adds two carbon atoms. A particular fatty acid chain undergoes many cycles until it achieves the ideal carbon chain length, as each the fatty acid in wax is generally composed of between 20 and 34 carbon units (Kunst and Samuels 2009).
In addition to the production of VLCFAs, waxes on the epidermis are mainly synthesized by the alcohol synthesis and alkane synthesis pathways (Kunst and Samuels 2009; Samuels et al. 2008). Primary alcohols and wax esters are produced by the alcohol synthesis pathway, with the WSD1 (a member of the bifunctional wax ester synthase/diacylglycerol acyltransferase gene family) having been shown to play a key role in the synthesis of wax esters in the stem of Arabidopsis thaliana (Li et al. 2008). Alkanes, secondary alcohols, aldehydes and ketones are produced by the alkane biosynthesis pathway. Here, ECERIFERUM 1 (CER1) and ECERIFERUM 3 (CER3) are known to be particularly important genes involved in the pathway of alkane synthesis in Arabidopsis (Bourdenx et al. 2011; Rowland et al. 2007).
Various components of wax are synthesized in the endoplasmic reticulum. Therefore, the waxes must pass through the plasma membrane to the apoplast and through the cell wall to reach the plant epidermis (Kunst and Samuels 2003). Previous studies have shown that acyl-CoA binding proteins (ACBPs) have a conserved acyl CoA binding (ACB) domain, which can bind to long-chain fatty acyl CoA. In Arabidopsis, acbp1 mutants had lower stem wax content than the wild type, suggesting that AtACBP1 is involved in the formation of the Arabidopsis stem epidermis through the transport of lipoyl CoA (Xue et al. 2014). ATP-BINDING CASSETTE G12 (ABCG12) and ATP-BINDING CASSETTE G11 (ABCG11), which encode two ATP-binding cassette (ABC) transporters, have also been shown to be involved in wax transport (Bird et al. 2007; Kunst and Samuels 2009; Pighin et al. 2004). Other studies have also shown that the genes encoding lipid transfer proteins (LTPs) are up-regulated during drought stress and that they induce cuticular wax accumulation, indicating that these proteins are also important to wax accumulation (Cameron et al. 2006).
The recent identification of these and other genes involved in wax synthesis is important, because wax synthesis is mainly controlled by regulation at the transcriptional and post-translational levels. Elucidation of the relevant genes supports the current focus on understanding transcriptional regulation. For example, several critical transcription factors, including members of the APETALA2 (AP2)/ethylene-responsive element binding factor (ERF/EREBP), MYB and homeodomain–leucine zipper (HD-ZIP) IV families have been found to regulate wax biosynthesis. In particular, with regard to the AP2 family, WAX INDUCER1/SHINE1 (WIN1/SHN1), WXP1, DECREASE WAX BIOSYNTHESIS (DEWAX) have been found to regulate wax biosynthesis (Aharoni et al. 2004; Go et al. 2014; Kannangara et al. 2007; Zhang et al. 2007). MYB106 and MYB16 can cooperate with WIN1/SHN1 to regulate wax synthesis (Oshima et al. 2013). In Arabidopsis and rice, CURLY FLAG LEAF1 (CFL1) interacts with HOMEODOMAIN GLABRA1 (HDG1) negatively regulates wax biosynthesis (Wu et al. 2011). Post translational regulation of proteins involved in wax synthesis has also been reported. For example, Oryza sativa DROUGHT HYPERSENSITIVE (OsDHS) has E3 ubiquitin ligase activity and promotes the degradation of the ROC4 (an HD-ZIP IV family member) via the 26S proteasome to negatively regulate the biosynthesis of wax and affect the drought resistance of rice (Wang et al. 2018). MIEL1 affects wax synthesis in Arabidopsis by activating the 26S proteasomal degradation pathway of MYB96 and MYB30 (Lee and Seo 2016; Marino et al. 2013). ECERIFERUM9 (CER9) gene in Arabidopsis encodes an E3 ubiquitin ligase, which regulates early steps of wax synthesis (Lu et al. 2012).
Thus, many enzyme and regulatory networks have been identified in epidermal wax synthesis pathways. However, few studies have focused on the comprehensive characterization of potential genes impacting apple cuticular wax biosynthesis. Here, we applied genome-wide association study (GWAS) analysis in a large sample of apples (123 accessions) to study the genetic basis for the diversity of leaf wax content through robust analysis of genotype-phenotype association. Our results demonstrated several new candidate chromosomal sites that may serve as the focus of future research on wax synthesis. This study provides a basis for molecular breeding to improve drought resistance of apples by improving wax accumulation and reducing water loss under drought conditions.
Results
Wax content of apple accessions
The wax contents of fresh leaves of 123 apple accessions were analyzed by gas chromatography-tandem mass spectrometry (GC-MS). These 123 apple accessions included 107 Malus domestica and 16 M. sieversii, of which four accessions were subspecies of M. sieversii f. neidzwetzkyana (Supplementary Table 1). Seventeen components in apple leaves were characterized, including alcohols, alkanes, fatty acids, and terpenes (Supplementary Table 2). The correlations among these 17 components were analyzed by Pearson correlation analysis (Fig. 1). The results showed that the first nine components were strongly correlated with each other. However, components 10, 11, 13, 16 and 17 were not strongly correlated with most other components (Fig. 1). In addition, the results showed that the contents of ursolic acid, oleanolic acid, hentriacontane and nonacosane were relatively high among the 17 components within the wax of apple leaves (Fig. 2).
Population structure and linkage disequilibrium
We sequenced the genomes of the 123 Malus accessions using the Illumina HiSeq 4000 sequencing platform, and a total of 803 Gb of raw sequence data were obtained. Trimming of low-quality reads and removing adapter sequences resulted in an average coverage of clean sequence data of approximately 16-fold for each accession. These sequences were aligned to a reference genome and used for calling of single nucleotide polymorphisms (SNPs). A total of 5.94 million SNPs were obtained with a missing rate less than 0.1 and a minor allele frequency (MAF) of at least 0.05. These SNPs were used for subsequent analyses.
Excluding SNPs with high linkage disequilibrium (LD) (r2 > 0.5), a subset of 115,802 SNPs, which were evenly distributed over the entire M. domestica GDDH13 genome, was used for population structure estimation. We performed phylogenetic tree construction of these 123 Malus accessions. The results suggested that they were divided into two major subpopulations (Fig. 3a), which is consistent with the known evolutionary history of the domesticated apple (Cornille et al. 2014; Duan et al. 2017).
Principal component analysis (PCA) was applied to simplify and summarize the genetic marker variation of the 123 accessions. The first three components of PCA (Fig. 3b) suggested a degree of genetic divergence in the two subpopulations (M. sieversii and M. domestica accessions). To further understand the population structure, we estimated ancestry proportions using a model-based method for each accession. For K = 2, M. domestica and M. sieversii accessions were clearly separated from each other (Fig. 3c).
The LD decayed rapidly in the two subpopulations, and the decay was faster in domesticated apple than in its ancestral species, M. sieversii. This relative rapidity of decay of LD in domesticated apples implies that the domestication bottleneck in cultivated apple is weak. In addition, because of the rapidly decaying LD, a higher density of genetic markers is required for association analyses.
GWAS analysis of 17 components of leaf wax
In order to explore the genetic basis of 17 components of wax in leaves, GWAS was performed by using the wax content data of the 123 apple accessions. According to the quantile-quantile (Q-Q) plot of the GWAS results, the p value observed in the lower left corner of the Q-Q plot was consistent with the expected p value. Therefore, we are confident that the p value obtained by the current statistical model conforms to the expected p value and that the statistical model is reasonable. In the upper right corner of the Q-Q plot are sites with high significance; these candidate sites are potentially associated with components (Supplementary Figs. 1 and 2).
The 17 components of the wax of apple leaves were analyzed to identify the key genes controlling the synthesis and transport of wax. Four components were of particular focus because they are of relatively high content within leaf wax. These four components are ursolic acid, oleanolic acid, hentriacontane and nonacosane. However, the GWAS results on ursolic acid were not further analyzed due to the large variance of the ursolic acid content. Therefore, our analysis focused on oleanolic acid, hentriacontane and nonacosane (Fig. 2).
The functions of genes associated with significant SNPs in the GWAS analysis were determined by Gene Ontology (GO) annotation, and GO annotation was similarly conducted for the three components. For these three components, the annotated genes were involved in biological processes, cellular components and molecular functions (Supplementary Figs. 3, 4, and 5). With regard to leaf wax nonacosane, 17 genes identified were associated with plant epidermis morphogenesis within the biological process category, and 12 identified genes were involved in the membrane protein complex in the cellular component category (Supplementary Fig. 3, Supplementary Table 3). For hentriacontane, some genes identified were associated with negative regulation of the developmental process and the fatty acid derivative biosynthetic process within the biological process category. Other genes were involved in transferase complexes and membrane protein complexes within cellular components (Supplementary Fig. 4, Supplementary Table 4). For oleanolic acid, some genes were involved in secondary metabolite biosynthetic processes, coenzyme biosynthetic processes, phenylpropanoid biosynthetic processes and phenylpropanoid metabolic processes in the biological process category (Supplementary Fig. 5, Supplementary Table 5).
Candidate genes for wax biosynthesis
Among the 17 components, three components were emphasized. Some known and new genes involved in the biosynthesis of wax in apple leaves were found (Figs. 4a, 5a, and 6a).
In the GWAS of leaf nonacosane, several significant SNPs were associated with some of the reported genes, including MdSHN1, MdLTP4 (LIPID TRANSFER PROTEIN4) and MdWSD1 (Cameron et al. 2006; Li et al. 2008; Oshima et al. 2013). One significant SNP was associated with gene MD02G1030900 on chromosome 2. This gene is homologous to AtSHN1. The product of AtSHN1, a member of the ERF/AP2 transcription factor family, induces the production of wax by involving in the alkane synthesis pathway. Over-expression of AtSHN1 makes leaves smooth and enhances drought tolerance of Arabidopsis (Aharoni et al. 2004). Twelve significant SNPs were located in MD09G1144900, which is homologous to AtLTP4. Three of these significant SNPs were located in the promoter region of the gene. The lipid transferase proteins encoded by AtLTP4 localize to the endoplasmic reticulum and bind fatty acids and acyl CoA esters in the process of lipid transport (Cameron et al. 2006). Another significant SNP was located in the promoter of a known wax synthesis-related gene, MD17G1237100, which is a homolog of AtWSD1-LIKE. This gene belongs to a member of the bifunctional wax ester synthase/diacylglycerol acyltransferase gene family (Li et al. 2008). Additionally, a significant SNP related to nonacosane was located in MD02G1087600, which is a homolog of the Arabidopsis ACYL-COA-BINDING PROTEIN6 (AtACBP6). To further verify the role of MdACBP6 in the synthesis of nonacosane, expression levels of MdACBP6 across 20 accessions were detected by real-time quantitative PCR (RT-qPCR). The results showed that the expression level of MdACBP6 was negatively correlated with the content of nonacosane, suggesting that MdACBP6 negatively regulates the accumulation of this component of leaf wax. There was a strong linear relationship between the content of nonacosane of leaf wax and the expression level of MdACBP6 (r2 = 0.611) (Fig. 4a and b, Supplementary Table 8). However, haplotype analysis showed no significant difference in wax content between the two haplotypes (Supplementary Fig. 7), indicating that the difference in expression level of MdACBP6 may be due to epigenetic modifications on promoters in different accessions.
Regarding hentriacontane, two significant SNPs located in the promoter of MD16G1017500 which is a homolog of the Arabidopsis gene that encodes RNA-DEPENDENT RNA POLYMERASE1 (AtRDR1). Studies by Lam et al. (2012) have shown that the product of the AtRDR1 gene, together with SUPPRESSOR OF GENE SILENCING3 (AtSGS3), regulates the production of epidermal wax in Arabidopsis by regulating the expression level of AtCER3. Additionally, four prominent SNPs were located in MD17G1287700. One of these four SNPs was the most prominent SNP on chromosome 17. MD17G1287700 is a homolog of Arabidopsis NOTCHLESS (AtNLE), which encodes a WD-40 repeat family protein. To further verify the role of MdNLE in the synthesis of hentriacontane, expression levels of MdNLE across 20 accessions were detected by RT-qPCR. These results showed that the expression level of MdNLE was negatively correlated with the content of hentriacontane, suggesting that MdNLE may negatively regulate the accumulation of hentriacontane in leaf wax. There was a strong linear relationship between the content of hentriacontane of leaf wax and the expression level of MdNLE (r2 = 0.755) (Fig. 5a and b, Supplementary Table 9). In order to investigate whether natural variations in MdNLE promoter contribute to the variation of its expression level, we performed haplotype analysis on MdNLE promoter. Results showed that the two haplotypes were separated based on the most prominent SNP on MdNLE promoter, with the Hap1 and Hap2 groups containing 87 and 31 accessions, respectively (Fig. 5c). Varieties carrying Hap1 showed a lower hentriacontane content than Hap2 varieties. We verified the existence of this SNP by Sanger sequencing with 40 accessions (Fig. 5d). The result indicated that the natural variation of MdNLE promoter may be the main effect SNP that causes the difference of hentriacontane content in different accessions.
With respect to the oleanolic acid, one SNP, which is the most significant SNP on chromosome 2, located in MD02G1211600 which is a homolog of AtLTP4. Moreover, on chromosome 16, one prominent SNP located in MD16G1050400, a homolog of an Arabidopsis gene that encodes an ATP-BINDING CASSETTE G21 (AtABCG21) transporter. Interestingly, this SNP, which is located in the coding sequence of MdABCG21, is the most prominent SNP on chromosome 16. To further analyze the potential role of MdABCG21 in the synthesis of oleanolic acid, expression levels of MdABCG21 across 20 accessions were detected by RT-qPCR. There was a strong positive linear relationship between the content of oleanolic acid and the expression level of MdABCG21 (r2 = 0.701), suggesting that MdABCG21 may be positively involved in the accumulation of oleanolic acid in leaf wax (Fig. 6a and b, Supplementary Table 10). Nevertheless, haplotype analysis showed no significant difference in wax content between the two haplotypes (Supplementary Fig. 8), indicating epigenetic modifications on promoters might be involved.
Discussion
In this study, 17 leaf wax components of 123 apple accessions were quantified (Supplementary Table 2). By exploring the correlations among these 17 wax components, we found that the first 9 components were strongly correlated, with correlation coefficients of 0.6 to 0.9, but the correlations of components 10, 13, 16, and 17 with other components were relatively weak (Fig. 1). Because the longest chain within the first nine components has 27 carbons, the strong correlation here may due to the fact that the FAE complex can only extend the carbon chain to 28 carbons (Haslam et al. 2015; Haslam et al. 2012; Pascal et al. 2013). The weak correlations of components 10, 13 with most other components may be due to the fact that the pathways leading to the synthesis of these components are alkane synthesis pathways, and carbon chains of components in this pathway have more than 28 carbons. The weak correlations of components 16 and 17 with most other components may be due to the fact that Oleanolic acid and Ursolic acid are terpenoids (Kunst and Samuels 2003).
Using phenotypic data from the leaves of 123 apple accessions, GWAS was performed on the wax content to identify genes involved in synthesis and transport of wax. We focused on the 17 prominent wax components identified in the GC-MS analysis (Supplementary Figs. 1 and 2). GO analysis was performed for all genes above threshold lines in GWAS of 17. These annotated genes were found to be involved in biological processes, cellular components, and molecular functions among the GO categories. The analysis indicated that 113 of the identified genes were associated with leaf development within biological processes, 79 genes were involved in vesicles within cellular components, 60 genes were associated with the Golgi subcompartment of cellular components and 52 genes were involved in the plasma membrane aspect of cellular components (Supplementary Fig. 6, Supplementary Table 6). The results are consistent with some hypotheses that wax transports from the ER to the plasma membrane as it is a hydrophobic substance and requires transport through the aqueous cytoplasm.
Three components were highlighted among the 17 wax components, including nonacosane, hentriacontane, and oleanolic acid. Previous studies have shown that the increase of leaf wax content under drought conditions is mainly due to the increase of long-chain alkanes, including nonacosane and hentriacontane. It shows that these two components of leaf wax play an important role in drought stress tolerance (Kosma et al. 2009; Panikashvili et al. 2007). Because of the key role played by the alkanes, it is of great significance to study these two components in apple leaf wax. In the GWAS of these three components, some genes associated with significant SNPs are known to be involved in wax synthesis and transport, including MdSHN1, MdLTP4, MdWSD1, and MdRDR1 (Cameron et al. 2006; Li et al. 2008; Lam et al. 2012; Oshima et al. 2013). We also identified some novel genes associated with significant SNPs, including MdACBP6, MdNLE, and MdABCG21 (Figs. 4, 5 and 6). The SNPs associated with these genes are located in the promoter, the coding sequence, downstream of the gene, or between genes. Notably, 20 significant SNPs related to nonacosane were found in MD14G1243300 on chromosome 14. This gene is homologous to HOMEODOMAIN GLABROUS11 (HDG11) in Arabidopsis, which belongs to the HD-ZIP IV family of homeobox-leucine zipper proteins. Previous studies have shown that the product of the HDG11 gene regulates the development of leaf trichomes in Arabidopsis (Khosla et al. 2014), while leaf trichomes and epidermal waxes have same precursors (Hegebarth et al. 2017). These connections suggest that MdHDG11 may regulate the epidermal wax in apple leaves, but this needs further analysis. There are two nonsynonymous mutations among the 20 significant SNPs associated with MdHDG11; these two significant SNPs may provide the basis for molecular breeding (Fig. 4a, Supplementary Table 8). We also found significant differences in wax content between the two haplotypes based on the most significant SNP in the promoters of MdLTP4, MdWSD1 and MdRDR1 (Supplementary Fig. 9), implying the potential usage of these SNPs in breeding in the future. More importantly, we identified a significant SNP in MdNLE promoter (Fig. 5c). Haplotype analysis and Sanger sequencing with 40 samples verified this SNP, indicating that MdNLE may be a key locus affecting wax of apple leaves (Fig. 5d).
However, there was no significant difference in wax content between the two haplotypes of MdACBP6 and MdABCG21, respectively (Supplementary Figs. 7 and 8). Studies have shown that dynamic chromatin environment has a great influence on gene expression in eukaryotic cells. Epigenetic modification, including chromatin accessibility and covalent modification of histone tails and DNA, can alter chromatin status to influence gene expression (He et al. 2015; Chang et al. 2020; Chen et al. 2020). Therefore, the difference in expression level of MdACBP6 and MdABCG21 may be due the epigenetic modification which affects the expression of these two genes needs to be further studied.
Studies have shown that wax synthesis is mainly controlled by regulation at transcriptional and post-translational levels. Therefore, various genes associated with significant SNPs in other components should also be further studied. These SNPs may perform different functions depending on their location on the associated genes. Taken together, our results provide a new direction for molecular breeding of apple trees with altered wax content.
Conclusion
In conclusion, we performed GWAS on leaf waxes of 123 Malus accessions. Seventeen major wax components and their contents were detected by GC-MS, and 5.9 million high-quality SNPs were identified by using whole-genome sequencing. Some important candidate genes, including MdSHN1, MdLTP4, MdWSD1, MdACBP6, MdRDR1, MdNLE, and MdABCG21, were identified which may play critical roles in the synthesis and transport of leaf waxes. These results provide hints for the mechanisms of wax synthesis and transport in apple leaves and for future resistance breeding.
Materials and methods
Samples for testing wax contents
Leaves of apple accessions (n = 123) were collected from the Horticulture Experimental Station of Northwest A&F University, Yangling, Shaanxi Province, China. Six leaves were collected from 3 trees of each accession.
Cuticular wax analysis of apple leaves
Wax extraction and measurement were described by Wang et al. (2018) with modifications. Briefly, leaves were steeped in 20 mL chloroform for 60 s, with 20 μl n-tetracosane (1 μg/μl) added as an internal standard. The chloroform extract containing wax was filtered through a 0.45 μm organic filter and dried with a stream of nitrogen. The residue was mixed with 40 μl pyridine and 40 μl N, O-bis (trimethylsilyl) trifluoroacetamide (BSTF) in a water bath at 70 °C for 60 min and then dried with a stream of nitrogen. The derivatized residue was redissolved in 1 mL chloroform and filtered through a 0.22 μm organic membrane. The extract was analyzed with GC-MS on a DB-5 MS column coupled with a Trace GC ULTRA/ISQ MS detector (Thermo Scientific, USA). Helium was used as the carrier gas of the column and split injection (10:1) was performed at 250 °C. The oven temperature was increased to 50 °C and kept at 50 °C for 2 min, increased at 20 °C min− 1 to 240 °C and kept at 240 °C for 2 min, and increased at 1.5 °C min− 1 to 320 °C and kept at 320 °C for 15 min. The total wax content was expressed per unit area of the blade surface. Leaf area was measured with an LI-3000C Portable Area Meter (LI-COR Biosciences).
The wax components were characterized by mass spectrometry combined with the mixed standard including C7-C40 saturated alkanes (CRM, Sigma, 49,452-U). The conversion of GC-MS amount data to the wax content was performed by the following formula:
A: Internal standard content (μg).
B: Peak area of sample.
C: Peak area of internal standard.
D: Leaf area (cm2).
Read alignment and SNP calling
The sequenced reads were derived from the apple genus resequecing dataset (Chen et al. 2021). The 123 Malus accessions which were used to analyze cuticular wax content were sequenced by whole-genome high-depth sequencing technology. Raw reads were trimmed using Trimmomatic (version 0.38) (Bolger, Lohse, & Usadel, 2014) with the following parameters: LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:40 TOPHRED33. Cleaned reads were mapped to the GDDH13 reference genome using BWA-MEM (version 0.7.17) (Li and Durbin 2009) with default parameters. Duplicated reads were marked and removed with Picard tools (version 2.1.1) (http://broadinstitute.github.io/picard).
SNP detection was performed using GATK (version 3.8) (McKenna et al. 2010) with “Best Practices” workflow for variant calling. SNPs that did not meet the following criteria were excluded: (a) biallelic alleles; (b) a total read depth greater than 150 and less than 3000; (c) minor allele frequency of at least 0.05; and (d) a maximum missing rate less than or equal to 0.1.
Population structure and LD analysis
For a more reasonable inference of the population structure, a total of 115,802 SNPs were randomly selected for population structure analysis. To understand the phylogenetic relationship, a distance matrix was generated by Plink (Chang et al. 2015) with parameter of ‘--distance-matrix’ and then a phylogeny tree was constructed using MEGA (version 10.2.4) (Kumar et al. 2018) with ‘Neighbor-Joining’ statistical method. PCA was performed using GCTA (version 1.01) (Yang et al. 2011). Population structure was investigated using ADMIXTURE (version 1.3) (Alexander and Lange 2011) which is a model-based clustering method for inferring population structure. We predefined genetic cluster (K) values from 2 to 6 and ran 20 replicates for each K to estimate the standard errors.
LD decay was calculated with PopLDdecay (version 3.41) (Zhang et al. 2019) with SNPs with a MAF of at least 0.05 and a maximum distance of 300 kb.
GWAS analysis
An Efficient Mixed-Model Association program, EMMAX, was used for the association analysis (Kang et al. 2010). The first three PCA values (eigenvectors), which were derived from whole-genome SNPs, were used as fixed effects. The random effect was estimated by kinship among all accessions. An IBS kinship matrix was derived from all SNPs with the EMMAX-Kin program. The cutoff was p = 1 × 10− 5.
Haplotype analysis
The significant SNPs in 2-kb promoter region of candidate genes were analyzed to haplotype phasing. Haplotypes in the LD blocks were calculated using the Haploview software with main parameters “-blockoutput GAB -blockCutHighCI 0.95 -blockCutLowCI 0.05 -minMAF 0.05”. Then, the accessions with wax content were divided into groups according to haplotypes, and Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test.
Total RNA extraction and RT-qPCR analysis
Total RNA was extracted from apple leaves by using CTAB and DNase I treatment. A sample of 1 μg of total RNA was reverse transcribed into cDNA using HiScriptIIQ RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech Co, Nanjing, China). RT-qPCR was performed using ChamQ SYBR qPCR Master Mix (Vazyme Biotech Co, Nanjing, China) with a Bio-Rad CFX real-time PCR detector. MdMDH was used as an internal reference gene for RT-qPCR.
GO (Gene Ontology) analysis
The SNPs associated with GWAS were annotated according to the apple reference genome GDDH13 (https://iris.angers.inra.fr/gddh13/) by using ANNOVAR software to obtain mutation sites and the identities of adjacent genes approximately 50 kb upstream and downstream of each SNP (Wang et al. 2010). BLASTP in NCBI-BLAST 2.9.0+ was used with the following key parameters. The E-value was equal to 1 × 10− 5, the suspension value of the best hit algorithm was 0.25, the edge value of the score of the best hit algorithm was 0.05, and protein sequences from The Arabidopsis Information Resource version 10 (TAIR10) were used as queries in BLAST searches. Genes associated with significant SNP were analyzed for GO enrichment by using the R package clusterProfiler (Yu et al. 2012).
Availability of data and materials
Whole genome sequences data of 123 Malus accessions was submitted to the Sequence Read Archive (SRA) database under the accession numbers PRJNA354212.
Abbreviations
- GC-MS:
-
Gas chromatography-tandem mass spectrometry
- SNPs:
-
Single nucleotide polymorphisms
- GWAS:
-
Genome-wide association study
- VLCFAs:
-
Very long chain fatty acids
- malonyl-ACP:
-
Malonyl-acyl carrier protein
- FAS:
-
Fatty acid synthase
- LACS:
-
Long-chain acyl coenzyme A synthetase
- ACC:
-
Acetyl-CoA carboxylase
- FAE:
-
Fatty acid elongase
- KCS:
-
β-Ketoacyl-CoA synthase
- KCR:
-
β-Ketoacyl-CoA reductase
- HCD:
-
β-Hydroxyacyl-CoA dehydratase
- ECR:
-
Enoyl-CoA reductase
- ACBPs:
-
Acyl-CoA binding proteins
- ACB:
-
Acyl CoA binding
- LTPs:
-
Lipid transfer proteins
- AP2:
-
APETALA2
- ERF/EREBP:
-
Ethylene-responsive element binding factor
- MAF:
-
Minor allele frequency
- LD:
-
Linkage disequilibrium
- PCA:
-
Principal component analysis
- Q-Q plot:
-
Quantile-quantile plot
- GO:
-
Gene Ontology
- RT-qPCR:
-
Real-time quantitative PCR
References
Aarts MG, Keijzer CJ, Stiekema WJ, Pereira A (1995) Molecular characterization of the CER1 gene of arabidopsis involved in epicuticular wax biosynthesis and pollen fertility. Plant Cell 7(12):2115–2127. https://doi.org/10.1105/tpc.7.12.2115
Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A (2004) The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis. Plant Cell 16(9):2463–2480. https://doi.org/10.1105/tpc.104.022897
Alexander DH, Lange K (2011) Enhancements to the ADMIXTURE algorithm for individual ancestry estimation. BMC Bioinformatics 12(1):246. https://doi.org/10.1186/1471-2105-12-246
Bach L, Michaelson LV, Haslam R, Bellec Y, Gissot L, Marion J, da Costa M, Boutin JP, Miquel M, Tellier F, Domergue F, Markham JE, Beaudoin F, Napier JA, Faure JD (2008) The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development. P Natl Acad Sci USA 105(38):14727–14731. https://doi.org/10.1073/pnas.0805089105
Beaudoin F et al (2009) Functional characterization of the Arabidopsis beta-ketoacyl-coenzyme a reductase candidates of the fatty acid elongase. Plant Physiol 150(3):1174–1191. https://doi.org/10.1104/pp.109.137497
Bird D, Beisson F, Brigham A, Shin J, Greer S, Jetter R, Kunst L, Wu X, Yephremov A, Samuels L (2007) Characterization of Arabidopsis ABCG11/WBC11, an ATP binding cassette (ABC) transporter that is required for cuticular lipid secretion. Plant J 52(3):485–498. https://doi.org/10.1111/j.1365-313X.2007.03252.x
Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics (Oxford, England) 30(15):2114–2120. https://doi.org/10.1093/bioinformatics/btu170
Bourdenx B, Bernard A, Domergue F, Pascal S, Léger A, Roby D, Pervent M, Vile D, Haslam RP, Napier JA, Lessire R, Joubès J (2011) Overexpression of Arabidopsis ECERIFERUM1 promotes wax very-long-chain alkane biosynthesis and influences plant response to biotic and abiotic stresses. Plant Physiol 156(1):29–45. https://doi.org/10.1104/pp.111.172320
Boyer J, Liu RH (2004) Apple phytochemicals and their health benefits. Nutr J 3(1):5. https://doi.org/10.1186/1475-2891-3-5
Buschhaus C, Jetter R (2011) Composition differences between epicuticular and intracuticular wax substructures: how do plants seal their epidermal surfaces? J Exp Bot 62(3):841–853. https://doi.org/10.1093/jxb/erq366
Cameron KD, Teece MA, Smart LB (2006) Increased accumulation of cuticular wax and expression of lipid transfer protein in response to periodic drying events in leaves of tree tobacco. Plant Physiol 140(1):176–183. https://doi.org/10.1104/pp.105.069724
Chang CC, Chow CC, Tellier LCAM, Vattikuti S, Purcell SM, Lee JJ (2015) Second-generation PLINK: rising to the challenge of larger and richer datasets. GigaScience 4:7 https://doi.org/10.1186/s13742-015-0047-8
Chang YN, Zhu C, Jiang J, Zhang HM, Zhu JK, Duan CG (2020) Epigenetic regulation in plant abiotic stress responses. J Integr Plant Biol 62(5):563–580 https://doi:10.1111/jipb.12901
Chen PX et al (2021) Insights into the effect of human civilization on Malus evolution and domestication. Plant Biotechnol J. https://doi.org/10.1111/pbi.13648. Advance online publication
Chen XS, Ding AB, Zhong XH (2020) Functions and mechanisms of plant histone deacetylases. Sci China Life Sci 63(2):206–216 https://doi:10.1007/s11427-019-1587-x
Cornille A, Giraud T, Smulders MJM, Roldan-Ruiz I, Gladieux P (2014) The domestication and evolutionary ecology of apples. Trends Genet 30(2):57–65. https://doi.org/10.1016/j.tig.2013.10.002
Dormann P, Voelker TA, Ohlrogge JB (1995) Cloning and expression in Escherichia coli of a novel thioesterase from Arabidopsis thaliana specific for long-chain acyl-acyl carrier proteins. Arch Biochem Biophys 316(1):612–618. https://doi.org/10.1006/abbi.1995.1081
Duan NB, Bai Y, Sun H, Wang N, Ma Y, Li M, Wang X, Jiao C, Legall N, Mao L, Wan S, Wang K, He T, Feng S, Zhang Z, Mao Z, Shen X, Chen X, Jiang Y, Wu S, Yin C, Ge S, Yang L, Jiang S, Xu H, Liu J, Wang D, Qu C, Wang Y, Zuo W, Xiang L, Liu C, Zhang D, Gao Y, Xu Y, Xu K, Chao T, Fazio G, Shu H, Zhong GY, Cheng L, Fei Z, Chen X (2017) Genome re-sequencing reveals the history of apple and supports a two-stage model for fruit enlargement. Nat Commun 8(1):249. https://doi.org/10.1038/s41467-017-00336-7
Go YS, Kim H, Kim HJ, Suh MC (2014) Arabidopsis Cuticular wax biosynthesis is negatively regulated by the DEWAX gene encoding an AP2/ERF-type transcription factor. Plant Cell 26(4):1666–1680. https://doi.org/10.1105/tpc.114.123307
Guo J, Xu W, Yu X, Shen H, Li H, Cheng D, Liu A, Liu J, Liu C, Zhao S, Song J (2016) Cuticular wax accumulation is associated with drought tolerance in wheat near-isogenic lines. Front Plant Sci 7:1809 https://doi.org/10.3389/fpls.2016.01809
Haslam TM, Haslam R, Thoraval D, Pascal S, Delude C, Domergue F, Fernández AM, Beaudoin F, Napier JA, Kunst L, Joubès J (2015) ECERIFERUM2-LIKE proteins have unique biochemical and physiological functions in very-long-chain fatty acid elongation. Plant Physiol 167(3):682–692. https://doi.org/10.1104/pp.114.253195
Haslam TM, Manas-Fernandez A, Zhao L, Kunst L (2012) Arabidopsis ECERIFERUM2 is a component of the fatty acid elongation machinery required for fatty acid extension to exceptional lengths. Plant Physiol 160(3):1164–1174. https://doi.org/10.1104/pp.112.201640
He ZX, Chen XW, Zhou ZW, Zhou SF (2015) Impact of physiological, pathological and environmental factors on the expression and activity of human cytochrome P450 2D6 and implications in precision medicine. Drug Metab Rev 47(4):470–519 https://doi:10.3109/03602532.2015.1101131
Hegebarth D, Buschhaus C, Joubes J, Thoraval D, Bird D, Jetter R (2017) Arabidopsis ketoacyl-CoA synthase 16 (KCS16) forms C36 /C38 acyl precursors for leaf trichome and pavement surface wax. Plant Cell Environ 40(9):1761–1776. https://doi.org/10.1111/pce.12981
Jones A, Davies HM, Voelker TA (1995) Palmitoyl-acyl carrier protein (Acp) Thioesterase and the evolutionary origin of plant acyl-Acp Thioesterases. Plant Cell 7(3):359–371. https://doi.org/10.1105/tpc.7.3.359
Kang HM, Sul JH, Service SK, Zaitlen NA, Kong SY, Freimer NB, Sabatti C, Eskin E (2010) Variance component model to account for sample structure in genome-wide association studies. Nat Genet 42(4):348–354. https://doi.org/10.1038/ng.548
Kannangara R, Branigan C, Liu Y, Penfield T, Rao V, Mouille Ǵ, Höfte H, Pauly M, Riechmann JL, Broun P (2007) The transcription factor WIN1/SHN1 regulates Cutin biosynthesis in Arabidopsis thaliana. Plant Cell 19(4):1278–1294. https://doi.org/10.1105/tpc.106.047076
Kerstiens G (2006) Water transport in plant cuticles: an update. J Exp Bot 57(11):2493–2499. https://doi.org/10.1093/jxb/erl017
Khosla A, Paper JM, Boehler AP, Bradley AM, Neumann TR, Schrick K (2014) HD-zip proteins GL2 and HDG11 have redundant functions in Arabidopsis Trichomes, and GL2 activates a positive feedback loop via MYB23. Plant Cell 26(5):2184–2200. https://doi.org/10.1105/tpc.113.120360
Kim J, Jung JH, Lee SB, Go YS, Kim HJ, Cahoon R, Markham JE, Cahoon EB, Suh MC (2013) Arabidopsis 3-ketoacyl-coenzyme a synthase9 is involved in the synthesis of tetracosanoic acids as precursors of cuticular waxes, suberins, sphingolipids, and phospholipids. Plant Physiol 162(2):567–580. https://doi.org/10.1104/pp.112.210450
Kosma DK, Bourdenx B, Bernard A, Parsons EP, Lu S, Joubes J, Jenks MA (2009) The impact of water deficiency on leaf cuticle lipids of Arabidopsis. Plant Physiol 151(4):1918–1929. https://doi.org/10.1104/pp.109.141911
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35(6):1547–1549. https://doi.org/10.1093/molbev/msy096
Kunst L, Samuels AL (2003) Biosynthesis and secretion of plant cuticular wax. Prog Lipid Res 42(1):51–80. https://doi.org/10.1016/S0163-7827(02)00045-0
Kunst L, Samuels L (2009) Plant cuticles shine: advances in wax biosynthesis and export. https://doi.org/10.1016/j.pbi.2009.09.009. 12(6):721–727
Lam P, Zhao L, McFarlane HE, Aiga M, Lam V, Hooker TS, Kunst L (2012) RDR1 and SGS3, components of RNA-mediated gene silencing, are required for the regulation of cuticular wax biosynthesis in developing inflorescence stems of Arabidopsis. Plant Physiol 159(4):1385–1395. https://doi.org/10.1104/pp.112.199646
Lee HG, Seo PJ (2016) The Arabidopsis MIEL1 E3 ligase negatively regulates ABA signalling by promoting protein turnover of MYB96. Nat Commun 7(1):12525. https://doi.org/10.1038/ncomms12525
Li F, Wu X, Lam P, Bird D, Zheng H, Samuels L, Jetter R, Kunst L (2008) Identification of the wax ester synthase/acyl-coenzyme a: diacylglycerol acyltransferase WSD1 required for stem wax ester biosynthesis in Arabidopsis. Plant Physiol 148(1):97–107. https://doi.org/10.1104/pp.108.123471
Li H, Durbin R (2009) Fast and accurate short read alignment with burrows-wheeler transform. Bioinformatics 25(14):1754–1760. https://doi.org/10.1093/bioinformatics/btp324
Liao P, Chen QF, Chye ML (2014) Transgenic Arabidopsis flowers overexpressing acyl-CoA-binding protein ACBP6 are freezing tolerant. Plant Cell Physiol 55(6):1055–1071. https://doi.org/10.1093/pcp/pcu037
Li-Beisson Y, Shorrosh B, Beisson F, Andersson MX, Arondel V, Bates PD, Baud S, Bird D, DeBono A, Durrett TP, Franke RB, Graham IA, Katayama K, Kelly AA, Larson T, Markham JE, Miquel M, Molina I, Nishida I, Rowland O, Samuels L, Schmid KM, Wada H, Welti R, Xu C, Zallot R, Ohlrogge J (2013) Acyl-lipid metabolism. Arabidopsis Book 11:e0161 https://doi.org/10.1199/tab.0161
Lu S, Song T, Kosma DK, Parsons EP, Rowland O, Jenks MA (2009) Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis. Plant J 59(4):553–564. https://doi.org/10.1111/j.1365-313X.2009.03892.x
Lu S et al (2011) The glossyhead1 allele of ACC1 reveals a principal role for multidomain acetyl-coenzyme a carboxylase in the biosynthesis of cuticular waxes by Arabidopsis. Plant Physiol 157(3):1079–1092. https://doi.org/10.1104/pp.111.185132
Lu SY et al (2012) Arabidopsis ECERIFERUM9 involvement in cuticle formation and maintenance of plant water status. Plant Physiol 159(3):930–944. https://doi.org/10.1104/pp.112.198697
Marino D, Froidure S, Canonne J, Ben Khaled S, Khafif M, Pouzet C, Jauneau A, Roby D, Rivas S (2013) Arabidopsis ubiquitin ligase MIEL1 mediates degradation of the transcription factor MYB30 weakening plant defence. Nat Commun 4(1):1476. https://doi.org/10.1038/ncomms2479
McKenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, Daly M, DePristo MA (2010) The genome analysis toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res 20(9):1297–1303. https://doi.org/10.1101/gr.107524.110
Mittelberger C, Yalcinkaya H, Pichler C, Gasser J, Scherzer G, Erhart T, Schumacher S, Holzner B, Janik K, Robatscher P, Müller T, Kräutler B, Oberhuber M (2017) Pathogen-induced leaf chlorosis: products of chlorophyll breakdown found in Degreened leaves of Phytoplasma-infected apple (Malus x domestica Borkh.) and apricot (Prunus armeniaca L.) trees relate to the Pheophorbide a oxygenase/Phyllobilin pathway. J Agric Food Chem 65(13):2651–2660. https://doi.org/10.1021/acs.jafc.6b05501
Oshima Y, Shikata M, Koyama T, Ohtsubo N, Mitsuda N, Ohme-Takagi M (2013) MIXTA-like transcription factors and WAX INDUCER1/SHINE1 coordinately regulate cuticle development in Arabidopsis and Torenia fournieri. Plant Cell 25(5):1609–1624. https://doi.org/10.1105/tpc.113.110783
Panikashvili D, Savaldi-Goldstein S, Mandel T, Yifhar T, Franke RB, Höfer Ŕ, Schreiber L, Chory J, Aharoni A (2007) The Arabidopsis DESPERADO/AtWBC11 transporter is required for cutin and wax secretion. Plant Physiol 145(4):1345–1360. https://doi.org/10.1104/pp.107.105676
Pascal S, Bernard A, Sorel M, Pervent M, Vile D, Haslam RP, Napier JA, Lessire R, Domergue F, Joubès J (2013) The Arabidopsis cer26 mutant, like the cer2 mutant, is specifically affected in the very long chain fatty acid elongation process. Plant J 73(5):733–746. https://doi.org/10.1111/tpj.12060
Pighin JA, Zheng H, Balakshin LJ, Goodman IP, Western TL, Jetter R, Kunst L, Samuels AL (2004) Plant cuticular lipid export requires an ABC transporter. Science 306(5696):702–704. https://doi.org/10.1126/science.1102331
Pollard M, Beisson F, Li Y, Ohlrogge JB (2008) Building lipid barriers: biosynthesis of cutin and suberin. Trends Plant Sci 13(5):236–246. https://doi.org/10.1016/j.tplants.2008.03.003
Rowland O, Lee R, Franke R, Schreiber L, Kunst L (2007) The CER3 wax biosynthetic gene from Arabidopsis thaliana is allelic to WAX2/YRE/FLP1. FEBS Lett 581(18):3538–3544. https://doi.org/10.1016/j.febslet.2007.06.065
Samuels L, Kunst L, Jetter R (2008) Sealing plant surfaces: Cuticular wax formation by epidermal cells. Annu Rev Plant Biol 59(1):683–707. https://doi.org/10.1146/annurev.arplant.59.103006.093219
Shepherd T, Wynne Griffiths D (2006) The effects of stress on plant cuticular waxes. New Phytol 171(3):469–499. https://doi.org/10.1111/j.1469-8137.2006.01826.x
Solovchenko A, Merzlyak M (2003) Optical properties and contribution of cuticle to UV protection in plants: experiments with apple fruit. Photochem Photobiol Sci 2(8):861–866. https://doi.org/10.1039/b302478d
Song T, Li K, Wu T, Wang Y, Zhang X, Xu X, Yao Y, Han Z (2019) Identification of new regulators through transcriptome analysis that regulate anthocyanin biosynthesis in apple leaves at low temperatures. PLoS One 14(1):e0210672. https://doi.org/10.1371/journal.pone.0210672
Troncoso-Ponce MA, Nikovics K, Marchive C, Lepiniec L, Baud S (2016) New insights on the organization and regulation of the fatty acid biosynthetic network in the model higher plant Arabidopsis thaliana. Biochimie 120:3–8 https://doi.org/10.1016/j.biochi.2015.05.013
Wang K, Li M, Hakonarson H (2010) ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res 38(16):e164. https://doi.org/10.1093/nar/gkq603
Wang Z, Benning C (2012) Chloroplast lipid synthesis and lipid trafficking through ER-plastid membrane contact sites. Biochem Soc Trans 40(2):457–463. https://doi.org/10.1042/BST20110752
Wang Z, Tian X, Zhao Q, Liu Z, Li X, Ren Y, Tang J, Fang J, Xu Q, Bu Q (2018) The E3 ligase DROUGHT HYPERSENSITIVE negatively regulates Cuticular wax biosynthesis by promoting the degradation of transcription factor ROC4 in Rice. Plant Cell 30(1):228–244. https://doi.org/10.1105/tpc.17.00823
Wu R, Li S, He S, Waßmann F, Yu C, Qin G, Schreiber L, Qu LJ, Gu H (2011) CFL1, a WW domain protein, regulates cuticle development by modulating the function of HDG1, a class IV homeodomain transcription factor, in rice and Arabidopsis. Plant Cell 23(9):3392–3411. https://doi.org/10.1105/tpc.111.088625
Xue Y, Xiao S, Kim J, Lung SC, Chen L, Tanner JA, Suh MC, Chye ML (2014) Arabidopsis membrane-associated acyl-CoA-binding protein ACBP1 is involved in stem cuticle formation. J Exp Bot 65(18):5473–5483. https://doi.org/10.1093/jxb/eru304
Yang J, Lee SH, Goddard ME, Visscher PM (2011) GCTA: a tool for genome-wide complex trait analysis. Am J Hum Genet 88(1):76–82. https://doi.org/10.1016/j.ajhg.2010.11.011
Yeats TH, Rose JK (2013) The formation and function of plant cuticles. Plant Physiol 163(1):5–20. https://doi.org/10.1104/pp.113.222737
Yu GC, Wang LG, Han YY, He QY (2012) clusterProfiler: an R package for comparing biological themes among gene clusters. Omics 16(5):284–287. https://doi.org/10.1089/omi.2011.0118
Zhang C, Dong SS, Xu JY, He WM, Yang TL (2019) PopLDdecay: a fast and effective tool for linkage disequilibrium decay analysis based on variant call format files. Bioinformatics 35(10):1786–1788. https://doi.org/10.1093/bioinformatics/bty875
Zhang JY, Broeckling CD, Sumner LW, Wang ZY (2007) Heterologous expression of two Medicago truncatula putative ERF transcription factor genes, WXP1 and WXP2, in Arabidopsis led to increased leaf wax accumulation and improved drought tolerance, but differential response in freezing tolerance. Plant Mol Biol 64(3):265–278. https://doi.org/10.1007/s11103-007-9150-2
Zhang M, Jin Z-Q, Zhao J, Zhang G, Wu F (2015) Physiological and biochemical responses to drought stress in cultivated and Tibetan wild barley. Plant Growth Regul 75(2):567–574. https://doi.org/10.1007/s10725-014-0022-x
Zheng H, Rowland O, Kunst L (2005) Disruptions of the Arabidopsis Enoyl-CoA reductase gene reveal an essential role for very-long-chain fatty acid synthesis in cell expansion during plant morphogenesis. Plant Cell 17(5):1467–1481. https://doi.org/10.1105/tpc.104.030155
Zhou L, Ni E, Yang J, Zhou H, Liang H, Li J, Jiang D, Wang Z, Liu Z, Zhuang C (2013) Rice OsGL1-6 is involved in leaf cuticular wax accumulation and drought resistance. PLoS One 8(5):e65139. https://doi.org/10.1371/journal.pone.0065139
Acknowledgments
We thank High Performance Computing (HPC) platform of Northwest A&F University (NWAFU) for providing computing resources.
Funding
This work was supported by the Key S&T Special Projects of Shaanxi Province, China (2020zdzx03–01-02).
Author information
Authors and Affiliations
Contributions
Q.G. and F.M. conceived the study. F.C., L.J., C.L., Q.Q. and F.Y. performed experiments. F.C. and Z.L. analyzed the data. F.C. and Z.L. wrote the manuscript. All authors read and approved the final manuscript.
Authors’ information
Fuguo Cao, Zhongxing Li, and Lijuan Jiang authors have contributed equally to this work.
Corresponding author
Ethics declarations
Competing interests
Author Q.G. is a member of the Editorial Board, but was not involved in the journal's review of, or decisions related to, this manuscript.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Additional file 1: Supplementary Table 1.
Details of all apple accessions. Supplementary Table 2. The contents of 17 wax components in leaves of 123 apple accessions (Each data is average of three biological replicates, unit: μg/cm2). Supplementary Table 3. Details of GO annotation of genes above the threshold line associated with nonacosane. Supplementary Table 4. Details of GO annotation of genes above the threshold line associated with hentriacontane. Supplementary Table 5. Details of GO annotation of genes above the threshold line associated with oleanolic acid. Supplementary Table 6. Details of GO annotation of genes above the threshold line associated with all components. Supplementary Table 7. List of all qPCR primers. Supplementary Table 8. List of significant loci from GWAS of nonacosane. Supplementary Table 9. List of significant loci from GWAS of hentriacontane. Supplementary Table 10. List of significant loci from GWAS of oleanolic acid. Supplementary Table 11. Gene ID information of the candidate genes for nonacosane. Supplementary Table 12. Gene ID information of the candidate genes for hentriacontane. Supplementary Table 13. Gene ID information of the candidate genes for oleanolic acid. Supplementary Table 14. Sanger sequencing primers of MdNLE promoter.
Additional file 2
: Supplementary Fig. 1. The GWAS results of different wax components of apple leaves (Components 1 to 8). The GWAS results of different wax components of apple leaves. Manhattan plots of GWAS is on the left. The chromosome number is plotted on the X-axis and the -log10 value of the p-value is plotted on Y-axis. The dotted lines indicate -log10(p) = 5. Quantile-quantile (Q-Q) plots of GWAS is on the right. The expected -log10 value of the p-value is on the X-axis and the observed -log10 value of the p-value is on Y-axis. Supplementary Fig. 2. The GWAS results of different wax components of apple leaves (Components 9 to 17). The GWAS results of different wax components of apple leaves. Manhattan plots of GWAS is on the left. The chromosome number is plotted on the X-axis, and the -log10 value of the p-value is plotted on Y-axis. The dotted lines indicate -log10(p) = 5. Quantile-quantile (Q-Q) plots of GWAS is on the right. The expected -log10 value of the p-value is on the X-axis and the observed -log10 value of the p-value is on Y-axis. Supplementary Fig. 3. Gene Ontology (GO) annotation of all associated genes above the threshold line from GWAS result of nonacosane. Supplementary Fig. 4. Gene Ontology (GO) annotation of all associated genes above the threshold line from GWAS result of hentriacontane. Supplementary Fig. 5. Gene Ontology (GO) annotation of all associated genes above the threshold line from GWAS result of oleanolic acid. Supplementary Fig. 6. GO (Gene Ontology) annotation of all associated genes above the threshold line from GWAS results of all components. Supplementary Fig. 7. Comparative analyses of MdACBP6 between the low and high nonacosane haplotypes according to the most significant SNP of the MdACBP6 promoter. Boxplot shows the content of nonacosane of each haplotype. The box expresses the upper, and the median and lower quartiles, and the dots represent extremes. Different letters indicate significant differences at p < 0.05 according to one-way analysis of variance (ANOVA). The table represents the location of the most significant SNP of MdACBP6 promoter and the accessions number corresponding to each haplotype. Supplementary Fig. 8. Comparative analyses of MdABCG21 between the low and high oleanolic acid haplotypes according to the most significant SNP of the MdABCG21 promoter. Boxplot shows the content of oleanolic acid of each haplotype. The box expresses the upper, and the median and lower quartiles, and the dots represent extremes. Different letters indicate significant differences at p < 0.05 according to one-way analysis of variance (ANOVA). The table represents the location of the most significant SNP of MdABCG21 promoter and the accessions number corresponding to each haplotype. Supplementary Fig. 9. Comparative analyses of MdLTP4, MdWSD1, and MdRDR1 between the low and high wax haplotypes according to the most significant SNP of their promoters. Boxplot shows the content of wax of each haplotype. The box expresses the upper, the median, and lower quartiles, and the dots represent extremes. Different letters indicate significant differences at p < 0.05 according to one-way analysis of variance (ANOVA). The table represents the location of the most significant SNP of their promoters and the accessions number corresponding to each haplotype.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Cao, F., Li, Z., Jiang, L. et al. Genome-wide association study (GWAS) of leaf wax components of apple. Stress Biology 1, 13 (2021). https://doi.org/10.1007/s44154-021-00012-3
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s44154-021-00012-3