Abstract
Natural antioxidants have attracted attention for their therapeutic use as photochemopreventive agents. Inga edulis leaves extract and its purified fraction have high polyphenolic content and high antioxidant capacity. In addition, they presented UV photostability and low citotoxicity in fibroblast cells. In this context, this study first aimed at development of topical formulation containing purified fraction of I. edulis extract and the evaluation of skin penetration of the compounds. Moreover, the photoprotective/photochemopreventive potential of the formulation containing I. edulis purified fraction were investigated in vitro and in vivo. The topical formulation containing 1% of the purified fraction of I. edulis increased the endogenous antioxidant potential of the skin, and vicenin-2 and myricetin compounds were able to penetrate the epidermis and dermis. Additionally, the purified fraction (25 and 50 mg/mL) showed a photoprotective effect against UVA and UVB radiation in L929 fibroblast cells. In vivo studies have shown that the formulation added with purified fraction provided an anti-inflammatory effect on the skin of animals after UVB exposure, since it was observed a reduction in MPO activity, IL-1β and TNF-α cytokines, and CXCL1/KC chemokine concentrations. In conclusion, the purified fraction of I. edulis, rich in phenolic compounds, when incorporated in topical formulation, appears as an alternative to prevent skin damages induced by UV radiation, due to its antioxidant and anti-inflammatory properties.
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References
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Acknowledgements
The authors thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for financial support. The authors also thank Professor Hervé Louis Ghislain Rogez from Federal University of Pará - Brazil for kindly providing the purified fraction of Inga edulis extract.
Funding
This work was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, Brazil [grant number 166315/2013-3] and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES, Brazil [grant number 001].
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KCC performed the experiments, collected the data and evaluated the results; FMPV and SAF performed the evaluation and interpretation of the data and the writing of the article; CHFC performed the evaluation and interpretation of the data; MJVF designed the study and performed the interpretation of the data and wrote the article.
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The Ethics Committee of the Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, approved the study with the use of animals under Protocol # 13.1.525.53.1.
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Costa, K.C., Cuelho, C.H.F., Figueiredo, S.A. et al. Photochemoprevention of topical formulation containing purified fraction of Inga edulis leaves extract. Photochem Photobiol Sci 22, 2105–2120 (2023). https://doi.org/10.1007/s43630-023-00433-1
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DOI: https://doi.org/10.1007/s43630-023-00433-1